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Literature summary extracted from

  • Hulse, J.D.; Henderson, L.M.
    Carnitine biosynthesis. Purification of 4-N-trimethylaminobutyraldehyde dehydrogenase from beef liver (1980), J. Biol. Chem., 255, 1146-1151.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.47 Disulfiram
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.47 0.00417
-
 4-trimethylaminobutyraldehyde
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.47 cytoplasm
-
 Bos taurus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.47 55000
-
 3 * 55000, SDS-PAGE Bos taurus
1.2.1.47 160000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.47 4-trimethylaminobutyraldehyde + NAD+ Bos taurus function in carnitine biosynthesis 4-trimethylaminobutyrate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.47 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.47 using column chromatography on blue dextran-Sepharose and 5'-AMP-Sepharose Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.47 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.47 0.21
-
 activity on propionaldehyde Bos taurus
1.2.1.47 5.1
-
 activity on 4-trimethylaminobutyraldehyde Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.2.1.47 -80°C, 0.1% 2-mercaptoethanol, 20% glycerol, several months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.47 4-trimethylaminobutyraldehyde + NAD+
-
 Bos taurus 4-trimethylaminobutyrate + NADH + H+
-
 ?
1.2.1.47 4-trimethylaminobutyraldehyde + NAD+ function in carnitine biosynthesis Bos taurus 4-trimethylaminobutyrate + NADH + H+
-
 ?
1.2.1.47 acetaldehyde + NAD+ + H2O low activity Bos taurus acetate + NADH + H+
-
 ?
1.2.1.47 butyraldehyde + NAD+ low activity Bos taurus butyrate + NADH
-
 ?
1.2.1.47 propionaldehyde + NAD+ low activity Bos taurus propionate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.47 trimer 3 * 55000, SDS-PAGE Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.47 4
-
 0.1% 2-mercaptoethanol, 20% glycerol, fairly stable, but storage for several weeks results in loss of most of the activity Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.47 9.5 9.8
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.47 6.2 10.5 pH 6.2: about 30% of activity maximum, pH 10.5: about 15% of activity maximum Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.47 NAD+ absolute requirement for Bos taurus