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Literature summary extracted from

  • Ogushi, S.; Ando, M.; Tsuru, D.
    Substrate specificity of formaldehyde dehydrogenase from Pseudomonas putida (1984), Agric. Biol. Chem., 48, 597-601.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.46 acetaldehyde substrate n-butanol Pseudomonas putida
1.2.1.46 ethanol substrate n-butanol Pseudomonas putida
1.2.1.46 n-butanol substrate formaldehyde Pseudomonas putida
1.2.1.46 n-Butyraldehyde substrate formaldehyde or n-butanol Pseudomonas putida
1.2.1.46 n-Hexanol substrate formaldehyde Pseudomonas putida
1.2.1.46 n-Propanol substrate n-butanol Pseudomonas putida
1.2.1.46 n-Valeraldehyde substrate n-butanol Pseudomonas putida
1.2.1.46 propionaldehyde substrate formaldehyde Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.46 additional information
-
 additional information Km for NAD+ with various aldehydes and alcohols Pseudomonas putida
1.2.1.46 0.09
-
 formaldehyde
-
 Pseudomonas putida
1.2.1.46 0.35
-
 NAD+ + formaldehyde Pseudomonas putida
1.2.1.46 4
-
 n-Hexanol
-
 Pseudomonas putida
1.2.1.46 4.2
-
 Isopentanol
-
 Pseudomonas putida
1.2.1.46 5.5
-
 benzyl alcohol
-
 Pseudomonas putida
1.2.1.46 5.9
-
 acetaldehyde
-
 Pseudomonas putida
1.2.1.46 6.2
-
 Isobutyraldehyde
-
 Pseudomonas putida
1.2.1.46 6.5
-
 n-Pentanol
-
 Pseudomonas putida
1.2.1.46 10.6
-
 n-butanol
-
 Pseudomonas putida
1.2.1.46 30
-
 isobutanol
-
 Pseudomonas putida
1.2.1.46 47
-
 propionaldehyde
-
 Pseudomonas putida
1.2.1.46 50
-
 n-Propanol
-
 Pseudomonas putida
1.2.1.46 100
-
 aryl alcohol
-
 Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.46 formaldehyde + NAD+ + H2O Pseudomonas putida enzyme of creatinine metabolism formate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.46 Pseudomonas putida
-
 C-83
-
1.2.1.46 Pseudomonas putida C-83
-
 C-83
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.46 C-83 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.46 additional information
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.46 acetaldehyde + NAD+ + H2O
-
 Pseudomonas putida acetate + NADH + H+
-
 ?
1.2.1.46 acetaldehyde + NAD+ + H2O
-
 Pseudomonas putida C-83 acetate + NADH + H+
-
 ?
1.2.1.46 aryl alcohol + NAD+ + H2O
-
 Pseudomonas putida ? + NADH
-
 ?
1.2.1.46 benzyl alcohol + NAD+ + H2O
-
 Pseudomonas putida ? + NADH
-
 ?
1.2.1.46 formaldehyde + NAD+ + H2O
-
 Pseudomonas putida formate + NADH
-
 ir
1.2.1.46 formaldehyde + NAD+ + H2O enzyme of creatinine metabolism Pseudomonas putida formate + NADH
-
 ?
1.2.1.46 isobutanol + NAD+ + H2O
-
 Pseudomonas putida ? + NADH
-
 ?
1.2.1.46 isobutyraldehyde + NAD+ + H2O
-
 Pseudomonas putida isobutyrate + NADH + H+
-
 r
1.2.1.46 isopentanol + NAD+ + H2O
-
 Pseudomonas putida ? + NADH
-
 ?
1.2.1.46 additional information specificity, best substrate: formaldehyde Pseudomonas putida ?
-
 ?
1.2.1.46 additional information reduced activity with increasing length of alkyl groups of aldehydes Pseudomonas putida ?
-
 ?
1.2.1.46 additional information no activity with n-butyraldehyde or n-valeraldehyde Pseudomonas putida ?
-
 ?
1.2.1.46 additional information no activity for methanol or ethanol Pseudomonas putida ?
-
 ?
1.2.1.46 additional information specificity, best substrate: formaldehyde Pseudomonas putida C-83 ?
-
 ?
1.2.1.46 additional information reduced activity with increasing length of alkyl groups of aldehydes Pseudomonas putida C-83 ?
-
 ?
1.2.1.46 additional information no activity with n-butyraldehyde or n-valeraldehyde Pseudomonas putida C-83 ?
-
 ?
1.2.1.46 additional information no activity for methanol or ethanol Pseudomonas putida C-83 ?
-
 ?
1.2.1.46 n-butanol + NAD+ + H2O
-
 Pseudomonas putida ? + NADH
-
 ?
1.2.1.46 n-hexanol + NAD+ + H2O
-
 Pseudomonas putida ? + NADH
-
 ?
1.2.1.46 n-pentanol + NAD+ + H2O
-
 Pseudomonas putida ? + NADH
-
 ?
1.2.1.46 n-propanol + NAD+ + H2O
-
 Pseudomonas putida ? + NADH
-
 ?
1.2.1.46 propionaldehyde + NAD+ + H2O
-
 Pseudomonas putida propionate + NADH
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.46 8.9
-
 formaldehyde Pseudomonas putida
1.2.1.46 10.8
-
 n-butanol Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.46 NAD+
-
 Pseudomonas putida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.46 0.27
-
 n-Butyraldehyde substrate formaldehyde Pseudomonas putida
1.2.1.46 1.9
-
 n-Valeraldehyde substrate n-butanol Pseudomonas putida
1.2.1.46 2.3
-
 n-Hexanol substrate formaldehyde Pseudomonas putida
1.2.1.46 2.6
-
 n-Butyraldehyde substrate n-butanol Pseudomonas putida
1.2.1.46 5.4
-
 n-butanol substrate formaldehyde Pseudomonas putida
1.2.1.46 6.1
-
 acetaldehyde substrate n-butanol Pseudomonas putida
1.2.1.46 35
-
 propionaldehyde substrate formaldehyde Pseudomonas putida
1.2.1.46 70
-
 n-Propanol substrate n-butanol Pseudomonas putida
1.2.1.46 90
-
 ethanol substrate n-butanol Pseudomonas putida