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Literature summary extracted from

  • Hohnloser, W.; Osswald, B.; Lingens, F.
    Enzymological aspects of caffeine demethylation and formaldehyde oxidation by Pseudomonas putida C1 (1980), Hoppe-Seyler's Z. Physiol. Chem., 361, 1763-1766.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.46 0.2
-
 formaldehyde
-
 Pseudomonas putida
1.2.1.46 0.5
-
 NAD+
-
 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.46 additional information independent of Mg2+ Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.46 250000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.46 Pseudomonas putida
-
-
-
1.2.1.46 Pseudomonas putida C1 / ATCC 17453
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.46 C1 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.46 8.3
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.46 formaldehyde + NAD+ + H2O
-
 Pseudomonas putida formate + NADH
-
 ?
1.2.1.46 formaldehyde + NAD+ + H2O
-
 Pseudomonas putida C1 / ATCC 17453 formate + NADH
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.46 30 35
-
 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.46 8.5 9
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.46 NAD+
-
 Pseudomonas putida