Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Guo, S.; Evans, S.A.; Wilkes, M.B.; Bhattacharjee, J.K.
    Novel posttranslational activation of the LYS2-encoded.alpha-aminoadipate reductase for biosynthesis of lysine and site-directed mutational analysis of conserved amino acid residues in the activation domain of Candida albicans (2001), J. Bacteriol., 183, 7120-7125.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.31 heterlogous expressed in Escherichia coli Candida albicans
1.2.1.95 expression in Escherichia coli Candida albicans

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.95 G881E conserved residue in the posttranslational activation domain LGGHSI, less than 50% of wild-type activity Candida albicans
1.2.1.95 G882R conserved residue in the posttranslational activation domain LGGHSI, loss of activity Candida albicans
1.2.1.95 G882S conserved residue in the posttranslational activation domain LGGHSI, loss of activity Candida albicans
1.2.1.95 H883R conserved residue in the posttranslational activation domain LGGHSI, strong reduction of activity Candida albicans
1.2.1.95 I885L G881E conserved residue in the posttranslational activation domain LGGHSI, less than 50% of wild-type activity Candida albicans
1.2.1.95 S884A conserved residue in the posttranslational activation domain LGGHSI, loss of activity Candida albicans
1.2.1.95 S884F conserved residue in the posttranslational activation domain LGGHSI, loss of activity Candida albicans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.95 150000
-
 x * 150000, SDS-PAGE Candida albicans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.31 L-alpha-aminoadipate + NAD(P)H Candida albicans
-
 L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.31 Candida albicans
-
-
-
1.2.1.95 Candida albicans Q12572
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.2.1.31 additional information specific amino acid residues, namely G882 and S884 of the Lys2p activation domain are required, the phosphopantetheinyl transferase is required for the activation of Lys2p and not for its alpha-aminoadipate reductase activity Candida albicans
1.2.1.31 side-chain modification the 150-kDa Lys2p is posttranslational modified in the presence of coenzyme A and Candida albicans lys2 mutant extract as a source of phosphopantetheinyl transferase Candida albicans
1.2.1.95 side-chain modification alpha-aminoadipate reductase Lys2 is posttranslationally activated by phosphopantetheinyl transferase Lys5 which transfers a phosphopantetheinyl group from coenzyme A to Lys2. Activation is required for catalytic activity Candida albicans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.31 additional information
-
-
 Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.31 L-alpha-aminoadipate + NAD(P)H
-
 Candida albicans L-alpha-aminoadipate-semialdehyde + NAD(P)+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.95 ? x * 150000, SDS-PAGE Candida albicans

Synonyms

EC Number Synonyms Comment Organism
1.2.1.95 LYS2
-
 Candida albicans