Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Calvert, A.F.; Rodwell, V.W.
    Metabolism of pipecolic acid in a Pseudomonas species. 3. L-alpha-aminoadipate delta-semialdehyde:nicotinamide adenine dinucleotide oxidoreductase (1966), J. Biol. Chem., 241, 409-414.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.31 acetaldehyde
-
 Pseudomonas sp.
1.2.1.31 alpha-aminoadipate
-
 Pseudomonas sp.
1.2.1.31 ATP 30% inhibition at 1 mM Pseudomonas sp.
1.2.1.31 Butyraldehyde
-
 Pseudomonas sp.
1.2.1.31 carbonyl reagents e.g.: hydroxylamine, KCN, in the presence of substrate Pseudomonas sp.
1.2.1.31 EDTA no inhibition observed Pseudomonas sp.
1.2.1.31 glutamate
-
 Pseudomonas sp.
1.2.1.31 HSO3- in presence of substrate Pseudomonas sp.
1.2.1.31 hydroxylamine in presence of substrate Pseudomonas sp.
1.2.1.31 imidazole 20% inhibition at 1 mM Pseudomonas sp.
1.2.1.31 KCN
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.31 0.002
-
 DELTA1-piperidine 6-carboxylate
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.31 additional information no metal cofactor required Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.31 Pseudomonas sp.
-
 P2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.31 using protamine sulfate treatment, ammonium sulfate fractionation and treatment with calcium phosphate gel Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.31 4.9
-
 after purification, gel fraction Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.31 (S)-2,3,4,5-tetrahydropyridine-2-carboxylate + NAD+ + H2O
-
 Pseudomonas sp. L-2-aminoadipate + NADH
-
 ir
1.2.1.31 DELTA1-piperidine 6-carboxylate + NAD+ + H2O
-
 Pseudomonas sp. L-alpha-aminoadipate + NADH + H+
-
 r
1.2.1.31 L-2-aminoadipate + NADPH in presence of ATP + Mg2+ Pseudomonas sp. L-2-aminoadipate 6-semialdehyde + NADP+ + H2O high specificity for L-alpha-aminoadipate-semialdehyde ?
1.2.1.31 L-2-aminoadipate 6-semialdehyde + NAD+ + H2O
-
 Pseudomonas sp. L-2-aminoadipate + NADH
-
 ir
1.2.1.31 additional information not: glutamate delta-semialdehyde DELTA1-pyrroline-5-carboxylate Pseudomonas sp. ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.31 8.2
-
 L-alpha-aminoadipate-semialdehyde reduction Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.31 6 9 pH 6: about 10% of activity maximum, pH 9: about 70% of activity maximum Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.31 additional information a variety of NAD+ analogues serve as electron acceptor: deamino-NAD+, NADP+, thionicotinamide-NAD+ and 3-acetylpyridine-NAD+ Pseudomonas sp.
1.2.1.31 NAD+
-
 Pseudomonas sp.