BRENDA - Enzyme Database show

Succinic semialdehyde dehydrogenase: purification and properties of the enzyme from monkey brain

Albers, R.W.; Koval, G.J.; Biochim. Biophys. Acta 52, 29-35 (1961)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.2.1.24
sodium lauryl sulfate
low concentrations activate to a small extent
Platyrrhini
General Stability
EC Number
General Stability
Organism
1.2.1.24
denaturation by sodium lauryl sulfate
Platyrrhini
1.2.1.24
NAD+ and mercaptoethanol effectively protect from auto-oxidation. NAD+ does not prevent auto-oxidation in presence of urea
Platyrrhini
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.24
4-hydroxybenzaldehyde
-
Platyrrhini
1.2.1.24
arsenite
-
Platyrrhini
1.2.1.24
m-hydroxybenzaldehyde
-
Platyrrhini
1.2.1.24
NEM
-
Platyrrhini
1.2.1.24
p-chloromercuriphenyl sulfonate
-
Platyrrhini
1.2.1.24
succinate semialdehyde
substrate inhibition above 0.02 mM
Platyrrhini
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.24
Platyrrhini
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.24
-
Platyrrhini
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.1.24
brain
-
Platyrrhini
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.24
succinate semialdehyde + NAD+ + H2O
-
288170
Platyrrhini
succinate + NADH
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.2.1.24
50
-
pH 7.4, 8 min, complete inactivation. Mercaptoethanol stabilizes for 20 min or longer
Platyrrhini
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.24
8.7
9.1
-
Platyrrhini
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.1.24
additional information
-
-
Platyrrhini
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.24
3-acetylpyridine adenine dinucleotide
reduced at nearly the same rate as NAD+
Platyrrhini
1.2.1.24
deamino-NAD+
reduced at nearly the same rate as NAD+
Platyrrhini
1.2.1.24
NAD+
-
Platyrrhini
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.2.1.24
sodium lauryl sulfate
low concentrations activate to a small extent
Platyrrhini
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.24
3-acetylpyridine adenine dinucleotide
reduced at nearly the same rate as NAD+
Platyrrhini
1.2.1.24
deamino-NAD+
reduced at nearly the same rate as NAD+
Platyrrhini
1.2.1.24
NAD+
-
Platyrrhini
General Stability (protein specific)
EC Number
General Stability
Organism
1.2.1.24
denaturation by sodium lauryl sulfate
Platyrrhini
1.2.1.24
NAD+ and mercaptoethanol effectively protect from auto-oxidation. NAD+ does not prevent auto-oxidation in presence of urea
Platyrrhini
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.24
4-hydroxybenzaldehyde
-
Platyrrhini
1.2.1.24
arsenite
-
Platyrrhini
1.2.1.24
m-hydroxybenzaldehyde
-
Platyrrhini
1.2.1.24
NEM
-
Platyrrhini
1.2.1.24
p-chloromercuriphenyl sulfonate
-
Platyrrhini
1.2.1.24
succinate semialdehyde
substrate inhibition above 0.02 mM
Platyrrhini
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.24
-
Platyrrhini
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.1.24
brain
-
Platyrrhini
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.24
succinate semialdehyde + NAD+ + H2O
-
288170
Platyrrhini
succinate + NADH
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.2.1.24
50
-
pH 7.4, 8 min, complete inactivation. Mercaptoethanol stabilizes for 20 min or longer
Platyrrhini
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.24
8.7
9.1
-
Platyrrhini
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.1.24
additional information
-
-
Platyrrhini