BRENDA - Enzyme Database show

Potato tuber succinate semialdehyde dehydrogenase: purification and characterization

Satya Narayan, V.; Madhusudanan Nair, P.; Arch. Biochem. Biophys. 275, 469-477 (1989)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.2.1.24
2-mercaptoethanol
activates
Solanum tuberosum
1.2.1.24
dithiothreitol
activates
Solanum tuberosum
1.2.1.24
glutathione
activates
Solanum tuberosum
1.2.1.24
L-Cys
activates
Solanum tuberosum
General Stability
EC Number
General Stability
Organism
1.2.1.24
freezing in absence of glycerol results in inactivation
Solanum tuberosum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.24
4-hydroxybenzaldehyde
-
Solanum tuberosum
1.2.1.24
acetaldehyde
1 mM, 40% inhibition
Solanum tuberosum
1.2.1.24
AMP
1 mM, 17% inhibition
Solanum tuberosum
1.2.1.24
arsenite
-
Solanum tuberosum
1.2.1.24
Cd2+
-
Solanum tuberosum
1.2.1.24
Cu2+
-
Solanum tuberosum
1.2.1.24
Dithionitrobenzoate
-
Solanum tuberosum
1.2.1.24
glyoxylate
1 mM, 33% inhibition
Solanum tuberosum
1.2.1.24
Hg2+
-
Solanum tuberosum
1.2.1.24
NADH
-
Solanum tuberosum
1.2.1.24
NEM
-
Solanum tuberosum
1.2.1.24
p-hydroxymercuribenzoate
-
Solanum tuberosum
1.2.1.24
Zn2+
-
Solanum tuberosum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.24
0.0046
-
succinate semialdehyde
-
Solanum tuberosum
1.2.1.24
0.031
-
NAD+
-
Solanum tuberosum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.24
35000
-
4 * 35000, SDS-PAGE
Solanum tuberosum
1.2.1.24
145000
-
gel filtration
Solanum tuberosum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.24
Solanum tuberosum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.24
-
Solanum tuberosum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.24
6.49
-
-
Solanum tuberosum
Storage Stability
EC Number
Storage Stability
Organism
1.2.1.24
-20°C, 20 mM sodium diphosphate buffer, pH 7.2, 14 mM 2-mercaptoethanol, 25% v/v glycerol, 2-3 months, retains 80-85% of the activity
Solanum tuberosum
1.2.1.24
0-2°C, 24 h, 50% loss of activity
Solanum tuberosum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.24
succinate semialdehyde + NAD+ + H2O
-
288150
Solanum tuberosum
succinate + NADH
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.1.24
tetramer
4 * 35000, SDS-PAGE
Solanum tuberosum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.24
9
-
-
Solanum tuberosum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.24
NAD+
-
Solanum tuberosum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.2.1.24
2-mercaptoethanol
activates
Solanum tuberosum
1.2.1.24
dithiothreitol
activates
Solanum tuberosum
1.2.1.24
glutathione
activates
Solanum tuberosum
1.2.1.24
L-Cys
activates
Solanum tuberosum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.24
NAD+
-
Solanum tuberosum
General Stability (protein specific)
EC Number
General Stability
Organism
1.2.1.24
freezing in absence of glycerol results in inactivation
Solanum tuberosum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.24
4-hydroxybenzaldehyde
-
Solanum tuberosum
1.2.1.24
acetaldehyde
1 mM, 40% inhibition
Solanum tuberosum
1.2.1.24
AMP
1 mM, 17% inhibition
Solanum tuberosum
1.2.1.24
arsenite
-
Solanum tuberosum
1.2.1.24
Cd2+
-
Solanum tuberosum
1.2.1.24
Cu2+
-
Solanum tuberosum
1.2.1.24
Dithionitrobenzoate
-
Solanum tuberosum
1.2.1.24
glyoxylate
1 mM, 33% inhibition
Solanum tuberosum
1.2.1.24
Hg2+
-
Solanum tuberosum
1.2.1.24
NADH
-
Solanum tuberosum
1.2.1.24
NEM
-
Solanum tuberosum
1.2.1.24
p-hydroxymercuribenzoate
-
Solanum tuberosum
1.2.1.24
Zn2+
-
Solanum tuberosum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.24
0.0046
-
succinate semialdehyde
-
Solanum tuberosum
1.2.1.24
0.031
-
NAD+
-
Solanum tuberosum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.24
35000
-
4 * 35000, SDS-PAGE
Solanum tuberosum
1.2.1.24
145000
-
gel filtration
Solanum tuberosum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.24
-
Solanum tuberosum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.24
6.49
-
-
Solanum tuberosum
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.2.1.24
-20°C, 20 mM sodium diphosphate buffer, pH 7.2, 14 mM 2-mercaptoethanol, 25% v/v glycerol, 2-3 months, retains 80-85% of the activity
Solanum tuberosum
1.2.1.24
0-2°C, 24 h, 50% loss of activity
Solanum tuberosum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.24
succinate semialdehyde + NAD+ + H2O
-
288150
Solanum tuberosum
succinate + NADH
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.1.24
tetramer
4 * 35000, SDS-PAGE
Solanum tuberosum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.24
9
-
-
Solanum tuberosum