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Literature summary extracted from

  • Hou, C.T.; Patel, R.N.; Laskin, A.I.; Barnabe, N.
    NAD-linked formate dehydrogenase from methanol-grown Pichia pastoris NRRL-Y-7556 (1982), Arch. Biochem. Biophys., 216, 296-305.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.17.1.9 both dithiothreitol, 10 mM, and glycerol, 10%, are required for stability during purification Komagataella pastoris

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.9 1,10-phenanthroline
-
 Komagataella pastoris
1.17.1.9 5-Nitro-8-hydroxyquinoline
-
 Komagataella pastoris
1.17.1.9 CN-
-
 [Candida] boidinii
1.17.1.9 CN- 1 mM KCN, 50% inhibition Komagataella pastoris
1.17.1.9 CN-
-
 Pseudomonas sp.
1.17.1.9 Cu2+
-
 [Candida] boidinii
1.17.1.9 Cu2+
-
 Komagataella pastoris
1.17.1.9 Dithionitrobenzoate
-
 Komagataella pastoris
1.17.1.9 Hg2+
-
 [Candida] boidinii
1.17.1.9 Hg2+
-
 Komagataella pastoris
1.17.1.9 iodoacetamide
-
 Komagataella pastoris
1.17.1.9 N3-
-
 [Candida] boidinii
1.17.1.9 N3-
-
 Komagataella pastoris
1.17.1.9 N3-
-
 Pseudomonas sp.
1.17.1.9 NEM
-
 Komagataella pastoris
1.17.1.9 p-hydroxymercuribenzoate
-
 Komagataella pastoris
1.17.1.9 Thiosemicarbazide
-
 Komagataella pastoris
1.17.1.9 Thiourea
-
 Komagataella pastoris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.9 0.14
-
 NAD+
-
 Komagataella pastoris
1.17.1.9 16
-
 formate
-
 Komagataella pastoris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.9 47000
-
 2 * 47000, SDS-PAGE Komagataella pastoris
1.17.1.9 94000
-
 gel filtration Komagataella pastoris

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 Komagataella pastoris
-
 NRRL Y-7556
-
1.17.1.9 Komagataella pastoris NRRL Y-7556
-
 NRRL Y-7556
-
1.17.1.9 Methylobacterium organophilum
-
-
-
1.17.1.9 Methylococcus capsulatus
-
-
-
1.17.1.9 Methylosinus trichosporium
-
-
-
1.17.1.9 Ogataea angusta
-
-
-
1.17.1.9 Pseudomonas sp.
-
-
-
1.17.1.9 Torulopsis candida
-
 NRRL Y-11419
-
1.17.1.9 Torulopsis candida NRRL Y-11419
-
 NRRL Y-11419
-
1.17.1.9 [Candida] boidinii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.9
-
 Komagataella pastoris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.9 8.2
-
-
 Komagataella pastoris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.9 formate + NAD+
-
 Pseudomonas sp. CO2 + NADH + H+
-
 ?
1.17.1.9 formate + NAD+
-
 Komagataella pastoris CO2 + NADH + H+
-
 ?
1.17.1.9 formate + NAD+
-
 [Candida] boidinii CO2 + NADH + H+
-
 ?
1.17.1.9 formate + NAD+
-
 Ogataea angusta CO2 + NADH + H+
-
 ?
1.17.1.9 formate + NAD+
-
 Torulopsis candida CO2 + NADH + H+
-
 ?
1.17.1.9 formate + NAD+
-
 Methylococcus capsulatus CO2 + NADH + H+
-
 ?
1.17.1.9 formate + NAD+
-
 Methylosinus trichosporium CO2 + NADH + H+
-
 ?
1.17.1.9 formate + NAD+
-
 Methylobacterium organophilum CO2 + NADH + H+
-
 ?
1.17.1.9 formate + NAD+
-
 Komagataella pastoris NRRL Y-7556 CO2 + NADH + H+
-
 ?
1.17.1.9 formate + NAD+
-
 Torulopsis candida NRRL Y-11419 CO2 + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.9 dimer
-
 [Candida] boidinii
1.17.1.9 dimer 2 * 47000, SDS-PAGE Komagataella pastoris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.1.9 20 25
-
 Komagataella pastoris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.17.1.9 55
-
 10 min, stable up to Komagataella pastoris
1.17.1.9 60
-
 10 min, complete loss of activity Komagataella pastoris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.9 6.5 7.5
-
 Komagataella pastoris
1.17.1.9 6.5 7.5
-
 Methylosinus trichosporium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.17.1.9 5.5 10 pH 5.5: about 25% of maximal activity, pH 10.0: about 40% of maximal activity Komagataella pastoris

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 NAD+
-
 Pseudomonas sp.
1.17.1.9 NAD+
-
 Komagataella pastoris
1.17.1.9 NAD+
-
 [Candida] boidinii
1.17.1.9 NAD+
-
 Ogataea angusta
1.17.1.9 NAD+
-
 Torulopsis candida
1.17.1.9 NAD+
-
 Methylococcus capsulatus
1.17.1.9 NAD+
-
 Methylosinus trichosporium
1.17.1.9 NAD+
-
 Methylobacterium organophilum