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Literature summary extracted from

  • Hoepner, T.; Ruschig, U.; Mueller, U.; Willnow, P.
    Formate dehydrogenase from Pseudomonas oxalaticus (1982), Methods Enzymol., 89, 531-537.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.9 CN-
-
 Cupriavidus oxalaticus
1.17.1.9 Hg2+
-
 Cupriavidus oxalaticus
1.17.1.9 N3- 0.25 mM. Inhibition is partly overcome by addition of formate or NAD+ Cupriavidus oxalaticus
1.17.1.9 NADH inhibition is partly overcome by addition of formate or NAD+ Cupriavidus oxalaticus
1.17.1.9 NO3- 0.25 mM. Inhibition is partly overcome by addition of formate or NAD+ Cupriavidus oxalaticus
1.17.1.9 p-hydroxymercuribenzoate
-
 Cupriavidus oxalaticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.9 0.105
-
 NAD+ formate dehydrogenase I, reaction with formate Cupriavidus oxalaticus
1.17.1.9 0.11
-
 NAD+ formate dehydrogenase II, reaction with formate Cupriavidus oxalaticus
1.17.1.9 0.13
-
 formate formate dehydrogenase II, reaction with NAD+ Cupriavidus oxalaticus
1.17.1.9 0.135
-
 formate formate dehydrogenase I, reaction with NAD+ Cupriavidus oxalaticus
1.17.1.9 0.2
-
 2,6-dichlorophenolindophenol formate dehydrogenase I or formate dehydrogenase II, reaction with NADH Cupriavidus oxalaticus
1.17.1.9 0.24
-
 benzyl viologen formate dehydrogenase II, reaction with formate Cupriavidus oxalaticus
1.17.1.9 0.25
-
 benzyl viologen formate dehydrogenase I, reaction with formate Cupriavidus oxalaticus
1.17.1.9 0.46
-
 ferricyanide formate dehydrogenase I, reaction with formate Cupriavidus oxalaticus
1.17.1.9 0.5
-
 ferricyanide formate dehydrogenase II, reaction with formate Cupriavidus oxalaticus
1.17.1.9 0.62
-
 ferricyanide formate dehydrogenase II, reaction with NADH Cupriavidus oxalaticus
1.17.1.9 0.69
-
 ferricyanide formate dehydrogenase I, reaction with NADH Cupriavidus oxalaticus
1.17.1.9 30 50 CO2
-
 Cupriavidus oxalaticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.1.9 Fe formate dehydrogenase I contains 18-25 mol of iron per mol of enzyme. Formate dehydrogenase II contains 6-10 mol of iron per mol of enzyme Cupriavidus oxalaticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.9 formate + NAD+ Cupriavidus oxalaticus when Pseudomonas oxalaticus is grown on formate as main carbon and energy source, formate dehydrogenase is the key enzyme that generates NADH and CO CO2 + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 Cupriavidus oxalaticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.9 essential conditions of purification are exclusion of light and oxygen Cupriavidus oxalaticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.9 32
-
-
 Cupriavidus oxalaticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.9 formate + 2,6-dichlorophenolindophenol
-
 Cupriavidus oxalaticus CO2 + reduced 2,6-dichlorophenolindophenol
-
 r
1.17.1.9 formate + FAD
-
 Cupriavidus oxalaticus CO2 + FADH2
-
 ?
1.17.1.9 formate + FMN
-
 Cupriavidus oxalaticus CO2 + FMNH2
-
 ?
1.17.1.9 formate + methyl viologen
-
 Cupriavidus oxalaticus CO2 + reduced methyl viologen
-
 ?
1.17.1.9 formate + methylene blue
-
 Cupriavidus oxalaticus CO2 + reduced methylene blue
-
 ?
1.17.1.9 formate + NAD+
-
 Cupriavidus oxalaticus CO2 + NADH + H+
-
 r
1.17.1.9 formate + NAD+ when Pseudomonas oxalaticus is grown on formate as main carbon and energy source, formate dehydrogenase is the key enzyme that generates NADH and CO Cupriavidus oxalaticus CO2 + NADH + H+
-
 ?
1.17.1.9 formate + nitrobluetetrazolium
-
 Cupriavidus oxalaticus CO2 + reduced nitrobluetetrazolium
-
 ?
1.17.1.9 formate + O2
-
 Cupriavidus oxalaticus CO2 + H2O2
-
 ?
1.17.1.9 formate + phenazine methosulfate
-
 Cupriavidus oxalaticus CO2 + reduced phenazine methosulfate
-
 ?
1.17.1.9 formate + riboflavin
-
 Cupriavidus oxalaticus CO2 + reduced riboflavin
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.9 6.6
-
 carbonate reduction by enzyme I Cupriavidus oxalaticus
1.17.1.9 7.4
-
 formate dehydrogenation, enzyme I Cupriavidus oxalaticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 FMN formate dehydrogenase I contains 1 mol of FMN per mol of enzyme, formate dehydrogenase II contains 2 mol of FMN per mol of enzyme Cupriavidus oxalaticus
1.17.1.9 NAD+
-
 Cupriavidus oxalaticus