EC Number | Application | Comment | Organism |
---|---|---|---|
1.4.3.10 | analysis | specific measurement of putrescine with putrescine oxidase and aminobutyraldehyde dehydrogenase | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.10 | putrescine | substrate inhibition at 10 mM or above | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.10 | 0.03 | - |
putrescine | - |
Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.19 | Pseudomonas putida | - |
A.T.C.C. 12633 | - |
1.4.3.10 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.19 | using column chromatography on DEAE-cellulose | Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.19 | additional information | - |
- |
Pseudomonas putida |
1.4.3.10 | additional information | - |
- |
Pseudomonas putida |
EC Number | Storage Stability | Organism |
---|---|---|
1.2.1.19 | -20°C, 25% glycerol, retaines almost full activity, one year | Pseudomonas putida |
1.4.3.10 | -20°C, in presence of 25% glycerol, 1 year, stable | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.19 | 4-aminobutanal + NAD+ + H2O | - |
Pseudomonas putida | 4-aminobutanoate + NADH + H+ | - |
? | |
1.2.1.19 | 5-aminovaleraldehyde + NAD+ + H2O | - |
Pseudomonas putida | 5-aminovalerate + NADH + H+ | - |
? | |
1.2.1.19 | additional information | N-acetyl-4-aminobutyraldehyde and 3-aminopropionaldehyde are poor substrates of the enzyme | Pseudomonas putida | ? | - |
? | |
1.2.1.19 | N-acetyl-3-aminopropionaldehyde + NAD+ + H2O | - |
Pseudomonas putida | N-acetyl-3-aminopropionate + NADH + H+ | - |
? | |
1.4.3.10 | cadaverine + O2 + H2O | at 11% of the activity with putrescine | Pseudomonas putida | 5-aminopentanal + NH3 + H2O2 | - |
? | |
1.4.3.10 | putrescine + O2 + H2O | - |
Pseudomonas putida | 4-aminobutanal + NH3 + H2O2 | - |
? | |
1.4.3.10 | spermidine + O2 + H2O | at 9% of the activity with putrescine | Pseudomonas putida | 4-aminobutyraldehyde + 1,3-diaminopropane + H2O2 | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.3.10 | 40 | - |
pH 7.5, 20 min, 17% loss of activity | Pseudomonas putida |
1.4.3.10 | 50 | - |
pH 7.5, 20 min, 62% loss of activity | Pseudomonas putida |
1.4.3.10 | 60 | - |
pH 7.5, 20 min, 93% loss of activity | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.3.10 | 8 | 11.7 | - |
Pseudomonas putida |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.4.3.10 | 6 | 10.5 | stable | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.19 | NAD+ | - |
Pseudomonas putida |