BRENDA - Enzyme Database

Intermediates and enzymes between alpha-ketoarginine and gamma-guanidinobutyrate in the L-arginine catabolic pathway of Pseudomonas putida

Vanderbilt, A.S.; Gaby, N.S.; Rodwell, V.W.; J. Biol. Chem. 250, 5322-5329 (1975)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.75
alpha-Hydroxy-delta-guanidino-N-pentanoate
weak
Pseudomonas putida
4.1.1.75
delta-Guanidinopentanoate
-
Pseudomonas putida
4.1.1.75
epsilon-Guanidinohexanoate
-
Pseudomonas putida
4.1.1.75
Ethylguanidine
-
Pseudomonas putida
4.1.1.75
gamma-Guanidinobutanoate
weak
Pseudomonas putida
4.1.1.75
gamma-guanidinobutyraldehyde
-
Pseudomonas putida
4.1.1.75
Guanidinoacetate
weak
Pseudomonas putida
4.1.1.75
Hexylguanidine
-
Pseudomonas putida
4.1.1.75
L-Arg
weak
Pseudomonas putida
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.19
0.15
-
NAD+
-
Pseudomonas putida
1.2.1.19
0.26
-
4-guanidinobutyraldehyde
-
Pseudomonas putida
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.1.19
cytoplasm
-
Pseudomonas putida
5737
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.1.75
Ca2+
activates, half-maximal activity at 0.6 mM
Pseudomonas putida
4.1.1.75
Co2+
activates, half-maximal activity at 0.3 mM
Pseudomonas putida
4.1.1.75
Mg2+
activates, half-maximal activity at 0.3 mM
Pseudomonas putida
4.1.1.75
Mn2+
activates, half-maximal activity at 0.03 mM
Pseudomonas putida
4.1.1.75
Ni2+
activates
Pseudomonas putida
4.1.1.75
Zn2+
activates
Pseudomonas putida
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.2.1.19
4-guanidinobutyraldehyde + NAD+ + H2O
Pseudomonas putida
involved in the L-arginine catabolism
4-guanidinobutyrate + NADH + H+
-
Pseudomonas putida
?
1.2.1.19
4-guanidinobutyraldehyde + NAD+ + H2O
Pseudomonas putida P2
involved in the L-arginine catabolism
4-guanidinobutyrate + NADH + H+
-
Pseudomonas putida P2
?
1.2.1.54
4-guanidinobutanal + NAD+
Pseudomonas putida
arginine catabolic pathway, involved in the degradation of arginine in Pseudomonas putida, cf. EC 1.2.1.19
4-guanidinobutanoate + NADH
-
-
-
4.1.1.75
5-Guanidino-2-oxopentanoate
Pseudomonas putida
induced by Arg or gamma-guanidinobutyraldehyde
?
-
-
-
4.1.1.75
5-Guanidino-2-oxopentanoate
Pseudomonas putida P2
induced by Arg or gamma-guanidinobutyraldehyde
?
-
-
-
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.2.1.19
Pseudomonas putida
-
A.T.C.C. 25571
-
1.2.1.54
Pseudomonas putida
-
inducible by L-arginine or its catabolic intermediates
-
4.1.1.75
Pseudomonas putida
-
-
-
4.1.1.75
Pseudomonas putida P2
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
4.1.1.75
-
Pseudomonas putida
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.19
0.009
-
cells grown on succinate and NH3
Pseudomonas putida
1.2.1.19
0.062
-
cells grown on L-glutamate
Pseudomonas putida
1.2.1.19
0.064
-
cells grown on 4-aminobutyric acid
Pseudomonas putida
1.2.1.19
0.148
-
cells grown on 4-guanidinobutyraldehyde
Pseudomonas putida
1.2.1.19
0.156
-
cells grown on 4-guanidinobutyric acid
Pseudomonas putida
1.2.1.19
0.33
-
cells grown on L-arginine
Pseudomonas putida
4.1.1.75
additional information
-
-
Pseudomonas putida
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.2.1.19
4-guanidinobutyraldehyde + NAD+ + H2O
-
288074
Pseudomonas putida
4-guanidinobutyrate + NADH + H+
-
288074
Pseudomonas putida
?
1.2.1.19
4-guanidinobutyraldehyde + NAD+ + H2O
involved in the L-arginine catabolism
288074
Pseudomonas putida
4-guanidinobutyrate + NADH + H+
-
288074
Pseudomonas putida
?
1.2.1.19
4-guanidinobutyraldehyde + NAD+ + H2O
-
288074
Pseudomonas putida P2
4-guanidinobutyrate + NADH + H+
-
288074
Pseudomonas putida P2
?
1.2.1.19
4-guanidinobutyraldehyde + NAD+ + H2O
involved in the L-arginine catabolism
288074
Pseudomonas putida P2
4-guanidinobutyrate + NADH + H+
-
288074
Pseudomonas putida P2
?
1.2.1.54
4-guanidinobutanal + NAD+
-
288074
Pseudomonas putida
4-guanidinobutanoate + NADH
-
-
-
?
1.2.1.54
4-guanidinobutanal + NAD+
arginine catabolic pathway, involved in the degradation of arginine in Pseudomonas putida, cf. EC 1.2.1.19
288074
Pseudomonas putida
4-guanidinobutanoate + NADH
-
-
-
-
1.2.1.54
4-guanidinobutanal + NAD+
-
288074
Pseudomonas putida P2
4-guanidinobutanoate + NADH
-
-
-
?
4.1.1.75
5-Guanidino-2-oxopentanoate
i.e. alpha-ketoarginine
288074
Pseudomonas putida
4-Guanidinobutyraldehyde + CO2
-
288074
Pseudomonas putida
-
4.1.1.75
5-Guanidino-2-oxopentanoate
i.e. alpha-ketoarginine
288074
Pseudomonas putida P2
4-Guanidinobutyraldehyde + CO2
-
288074
Pseudomonas putida P2
-
4.1.1.75
5-Guanidino-2-oxopentanoate
induced by Arg or gamma-guanidinobutyraldehyde
288074
Pseudomonas putida
?
-
-
-
-
4.1.1.75
5-Guanidino-2-oxopentanoate
induced by Arg or gamma-guanidinobutyraldehyde
288074
Pseudomonas putida P2
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.1.75
70
-
-
Pseudomonas putida
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.2.1.54
80
-
10 min, complete inactivation
Pseudomonas putida
4.1.1.75
80
-
30 min, stable
Pseudomonas putida
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.1.75
167
-
5-guanidino-2-oxopentanoate
-
Pseudomonas putida
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.19
8.5
9
-
Pseudomonas putida
1.2.1.54
8.5
9
-
Pseudomonas putida
4.1.1.75
8.5
-
-
Pseudomonas putida
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.1.19
7
10
-
Pseudomonas putida
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.19
additional information
not NADP+
Pseudomonas putida
1.2.1.19
NAD+
highly specific for
Pseudomonas putida
1.2.1.54
NAD+
highly specific for
Pseudomonas putida
4.1.1.75
NAD+
required
Pseudomonas putida
4.1.1.75
thiamine diphosphate
required
Pseudomonas putida
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.19
additional information
not NADP+
Pseudomonas putida
1.2.1.19
NAD+
highly specific for
Pseudomonas putida
1.2.1.54
NAD+
highly specific for
Pseudomonas putida
4.1.1.75
NAD+
required
Pseudomonas putida
4.1.1.75
thiamine diphosphate
required
Pseudomonas putida
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.75
alpha-Hydroxy-delta-guanidino-N-pentanoate
weak
Pseudomonas putida
4.1.1.75
delta-Guanidinopentanoate
-
Pseudomonas putida
4.1.1.75
epsilon-Guanidinohexanoate
-
Pseudomonas putida
4.1.1.75
Ethylguanidine
-
Pseudomonas putida
4.1.1.75
gamma-Guanidinobutanoate
weak
Pseudomonas putida
4.1.1.75
gamma-guanidinobutyraldehyde
-
Pseudomonas putida
4.1.1.75
Guanidinoacetate
weak
Pseudomonas putida
4.1.1.75
Hexylguanidine
-
Pseudomonas putida
4.1.1.75
L-Arg
weak
Pseudomonas putida
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.19
0.15
-
NAD+
-
Pseudomonas putida
1.2.1.19
0.26
-
4-guanidinobutyraldehyde
-
Pseudomonas putida
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.1.19
cytoplasm
-
Pseudomonas putida
5737
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.1.75
Ca2+
activates, half-maximal activity at 0.6 mM
Pseudomonas putida
4.1.1.75
Co2+
activates, half-maximal activity at 0.3 mM
Pseudomonas putida
4.1.1.75
Mg2+
activates, half-maximal activity at 0.3 mM
Pseudomonas putida
4.1.1.75
Mn2+
activates, half-maximal activity at 0.03 mM
Pseudomonas putida
4.1.1.75
Ni2+
activates
Pseudomonas putida
4.1.1.75
Zn2+
activates
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.2.1.19
4-guanidinobutyraldehyde + NAD+ + H2O
Pseudomonas putida
involved in the L-arginine catabolism
4-guanidinobutyrate + NADH + H+
-
Pseudomonas putida
?
1.2.1.19
4-guanidinobutyraldehyde + NAD+ + H2O
Pseudomonas putida P2
involved in the L-arginine catabolism
4-guanidinobutyrate + NADH + H+
-
Pseudomonas putida P2
?
1.2.1.54
4-guanidinobutanal + NAD+
Pseudomonas putida
arginine catabolic pathway, involved in the degradation of arginine in Pseudomonas putida, cf. EC 1.2.1.19
4-guanidinobutanoate + NADH
-
-
-
4.1.1.75
5-Guanidino-2-oxopentanoate
Pseudomonas putida
induced by Arg or gamma-guanidinobutyraldehyde
?
-
-
-
4.1.1.75
5-Guanidino-2-oxopentanoate
Pseudomonas putida P2
induced by Arg or gamma-guanidinobutyraldehyde
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.75
-
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.19
0.009
-
cells grown on succinate and NH3
Pseudomonas putida
1.2.1.19
0.062
-
cells grown on L-glutamate
Pseudomonas putida
1.2.1.19
0.064
-
cells grown on 4-aminobutyric acid
Pseudomonas putida
1.2.1.19
0.148
-
cells grown on 4-guanidinobutyraldehyde
Pseudomonas putida
1.2.1.19
0.156
-
cells grown on 4-guanidinobutyric acid
Pseudomonas putida
1.2.1.19
0.33
-
cells grown on L-arginine
Pseudomonas putida
4.1.1.75
additional information
-
-
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.2.1.19
4-guanidinobutyraldehyde + NAD+ + H2O
-
288074
Pseudomonas putida
4-guanidinobutyrate + NADH + H+
-
288074
Pseudomonas putida
?
1.2.1.19
4-guanidinobutyraldehyde + NAD+ + H2O
involved in the L-arginine catabolism
288074
Pseudomonas putida
4-guanidinobutyrate + NADH + H+
-
288074
Pseudomonas putida
?
1.2.1.19
4-guanidinobutyraldehyde + NAD+ + H2O
-
288074
Pseudomonas putida P2
4-guanidinobutyrate + NADH + H+
-
288074
Pseudomonas putida P2
?
1.2.1.19
4-guanidinobutyraldehyde + NAD+ + H2O
involved in the L-arginine catabolism
288074
Pseudomonas putida P2
4-guanidinobutyrate + NADH + H+
-
288074
Pseudomonas putida P2
?
1.2.1.54
4-guanidinobutanal + NAD+
-
288074
Pseudomonas putida
4-guanidinobutanoate + NADH
-
-
-
?
1.2.1.54
4-guanidinobutanal + NAD+
arginine catabolic pathway, involved in the degradation of arginine in Pseudomonas putida, cf. EC 1.2.1.19
288074
Pseudomonas putida
4-guanidinobutanoate + NADH
-
-
-
-
1.2.1.54
4-guanidinobutanal + NAD+
-
288074
Pseudomonas putida P2
4-guanidinobutanoate + NADH
-
-
-
?
4.1.1.75
5-Guanidino-2-oxopentanoate
i.e. alpha-ketoarginine
288074
Pseudomonas putida
4-Guanidinobutyraldehyde + CO2
-
288074
Pseudomonas putida
-
4.1.1.75
5-Guanidino-2-oxopentanoate
i.e. alpha-ketoarginine
288074
Pseudomonas putida P2
4-Guanidinobutyraldehyde + CO2
-
288074
Pseudomonas putida P2
-
4.1.1.75
5-Guanidino-2-oxopentanoate
induced by Arg or gamma-guanidinobutyraldehyde
288074
Pseudomonas putida
?
-
-
-
-
4.1.1.75
5-Guanidino-2-oxopentanoate
induced by Arg or gamma-guanidinobutyraldehyde
288074
Pseudomonas putida P2
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.1.75
70
-
-
Pseudomonas putida
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.2.1.54
80
-
10 min, complete inactivation
Pseudomonas putida
4.1.1.75
80
-
30 min, stable
Pseudomonas putida
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.1.75
167
-
5-guanidino-2-oxopentanoate
-
Pseudomonas putida
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.19
8.5
9
-
Pseudomonas putida
1.2.1.54
8.5
9
-
Pseudomonas putida
4.1.1.75
8.5
-
-
Pseudomonas putida
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.1.19
7
10
-
Pseudomonas putida