BRENDA - Enzyme Database

4-Aminobutyraldehyde and 4-guanidinobutyraldehyde dehydrogenase for arginine degradation in Pseudomonas putida

Yorifuji, T.; Koike, K.; Sakurai, T.; Yokoyama, K.; Agric. Biol. Chem. 50, 2009-2016 (1986)
No PubMed abstract available

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.2.1.19
2-mercaptoethanol and NAD+, no stabilization
Pseudomonas putida
1.2.1.54
inactive in absence of 2-mercaptoethanol
Pseudomonas putida
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.19
2-Carboxy-2'-hydroxy-5'-sulfoformazylbenzene
zincon
Pseudomonas putida
1.2.1.19
iodoacetamide
-
Pseudomonas putida
1.2.1.19
monoiodoacetate
slowly
Pseudomonas putida
1.2.1.19
N-ethylmaleimide
-
Pseudomonas putida
1.2.1.19
p-chloromercuribenzoate
-
Pseudomonas putida
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.19
0.013
-
Valeraldehyde
-
Pseudomonas putida
1.2.1.19
0.016
-
Butyraldehyde
-
Pseudomonas putida
1.2.1.19
0.13
-
4-guanidinobutyraldehyde
pH 9.5
Pseudomonas putida
1.2.1.19
0.16
-
4-guanidinobutyraldehyde
pH 8.0
Pseudomonas putida
1.2.1.19
0.2
-
5-Aminovaleraldehyde
-
Pseudomonas putida
1.2.1.19
0.26
-
4-Aminobutyraldehyde
pH 8.0
Pseudomonas putida
1.2.1.19
0.83
-
4-Aminobutyraldehyde
pH 9.5
Pseudomonas putida
1.2.1.54
0.03
-
4-guanidinobutyraldehyde
-
Pseudomonas putida
1.2.1.54
0.14
-
NAD+
-
Pseudomonas putida
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.19
57000
-
4 * 57000, SDS-PAGE
Pseudomonas putida
1.2.1.19
83000
-
gel filtration
Pseudomonas putida
1.2.1.19
240000
-
gel filtration
Pseudomonas putida
1.2.1.54
57000
-
2 * 57000, SDS-PAGE
Pseudomonas putida
1.2.1.54
107000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.2.1.19
4-aminobutanal + NAD+ + H2O
Pseudomonas putida
involved in the arginine decarboxylase pathway
4-aminobutanoate + NADH + H+
-
Pseudomonas putida
?
1.2.1.54
4-guanidinobutanal + NAD+
Pseudomonas putida
arginine catabolic pathway, involved in the degradation of arginine in Pseudomonas putida, cf. EC 1.2.1.19
4-guanidinobutanoate + NADH
-
-
-
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.2.1.19
Pseudomonas putida
-
A.T.C.C. 12633
-
1.2.1.54
Pseudomonas putida
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.2.1.19
using column chromatography on DEAE-cellulose, DEAE-Toyopearl, Toyopearl HW-55 and Affi-Gel Blue
Pseudomonas putida
1.2.1.54
-
Pseudomonas putida
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.19
additional information
-
-
Pseudomonas putida
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.2.1.19
3-aminopropanal + NAD+ + H2O
-
288069
Pseudomonas putida
3-aminopropanoate + NADH + H+
-
288069
Pseudomonas putida
?
1.2.1.19
4-aminobutanal + NAD+ + H2O
-
288069
Pseudomonas putida
4-aminobutanoate + NADH + H+
-
288069
Pseudomonas putida
?
1.2.1.19
4-aminobutanal + NAD+ + H2O
involved in the arginine decarboxylase pathway
288069
Pseudomonas putida
4-aminobutanoate + NADH + H+
-
288069
Pseudomonas putida
?
1.2.1.19
4-guanidinobutyraldehyde + NAD+ + H2O
-
288069
Pseudomonas putida
4-guanidinobutyrate + NADH + H+
-
288069
Pseudomonas putida
?
1.2.1.19
4-ureidobutyraldehyde + NAD+
-
288069
Pseudomonas putida
4-ureidobutyrate + NADH + H+
-
288069
Pseudomonas putida
?
1.2.1.19
5-aminovaleraldehyde + NAD+ + H2O
-
288069
Pseudomonas putida
5-aminovalerate + NADH + H+
-
288069
Pseudomonas putida
?
1.2.1.19
butyraldehyde + NAD+ + H2O
-
288069
Pseudomonas putida
butyrate + NADH + H+
-
288069
Pseudomonas putida
?
1.2.1.19
propionaldehyde + NAD+ + H2O
-
288069
Pseudomonas putida
propionate + NADH + H+
-
288069
Pseudomonas putida
?
1.2.1.19
valeraldehyde + NAD+ + H2O
-
288069
Pseudomonas putida
valerate + NADH + H+
-
288069
Pseudomonas putida
?
1.2.1.54
4-guanidinobutanal + NAD+
specific for
288069
Pseudomonas putida
4-guanidinobutanoate + NADH
-
-
-
?
1.2.1.54
4-guanidinobutanal + NAD+
arginine catabolic pathway, involved in the degradation of arginine in Pseudomonas putida, cf. EC 1.2.1.19
288069
Pseudomonas putida
4-guanidinobutanoate + NADH
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.2.1.19
tetramer
4 * 57000, SDS-PAGE
Pseudomonas putida
1.2.1.54
dimer
2 * 57000, SDS-PAGE
Pseudomonas putida
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.2.1.19
30
-
30 min, pH 5.0-8.0, stable; for 30 min, in 100 mM K-phosphate buffer, pH 7, allmost all activity retained; for 30 min, in 100 mM Na-acetate buffer, pH 5, allmost all activity retained; for 30 min, in 100 mM Na-bicine buffer, pH 8, allmost all activity retained
Pseudomonas putida
1.2.1.19
60
-
for 15 min, in 100 mM K-phosphate buffer, pH 7, 35% of activity lost; pH 7.0, 15 min, 35% loss of activity
Pseudomonas putida
1.2.1.54
40
-
15 min, pH 8.0, 70% loss of activity
Pseudomonas putida
1.2.1.54
50
-
15 min, pH 8.0, 100% loss of activity
Pseudomonas putida
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.19
8
-
optimum toward 4-aminobutyraldehyde
Pseudomonas putida
1.2.1.19
8.5
9.5
optimum toward 4-guanidinobutyraldehyde and 5-aminovaleraldehyde
Pseudomonas putida
1.2.1.54
9
-
-
Pseudomonas putida
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.1.19
6.5
9
4-aminobutyraldehyde, at pH 6.5: about 30% of activity maximum, at pH 9.0: about 70% of activity maximum
Pseudomonas putida
1.2.1.19
7
10
4-guanidinobutyraldehyde, at pH 7.0: about 35% of activity maximum, at pH 10.0: about 50% of activity maximum
Pseudomonas putida
1.2.1.54
7
10
pH 7.0: about 20% of activity maximum, pH 10.0: about 80% of activity maximum
Pseudomonas putida
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.2.1.19
7
-
most stable at neutral pH
Pseudomonas putida
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.19
NAD+
highly specific for
Pseudomonas putida
1.2.1.19
NADP+
rate of dehydrogenation about 4% those with NAD+
Pseudomonas putida
1.2.1.54
NAD+
highly specific for
Pseudomonas putida
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.19
NAD+
highly specific for
Pseudomonas putida
1.2.1.19
NADP+
rate of dehydrogenation about 4% those with NAD+
Pseudomonas putida
1.2.1.54
NAD+
highly specific for
Pseudomonas putida
General Stability (protein specific)
EC Number
General Stability
Organism
1.2.1.19
2-mercaptoethanol and NAD+, no stabilization
Pseudomonas putida
1.2.1.54
inactive in absence of 2-mercaptoethanol
Pseudomonas putida
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.19
2-Carboxy-2'-hydroxy-5'-sulfoformazylbenzene
zincon
Pseudomonas putida
1.2.1.19
iodoacetamide
-
Pseudomonas putida
1.2.1.19
monoiodoacetate
slowly
Pseudomonas putida
1.2.1.19
N-ethylmaleimide
-
Pseudomonas putida
1.2.1.19
p-chloromercuribenzoate
-
Pseudomonas putida
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.19
0.013
-
Valeraldehyde
-
Pseudomonas putida
1.2.1.19
0.016
-
Butyraldehyde
-
Pseudomonas putida
1.2.1.19
0.13
-
4-guanidinobutyraldehyde
pH 9.5
Pseudomonas putida
1.2.1.19
0.16
-
4-guanidinobutyraldehyde
pH 8.0
Pseudomonas putida
1.2.1.19
0.2
-
5-Aminovaleraldehyde
-
Pseudomonas putida
1.2.1.19
0.26
-
4-Aminobutyraldehyde
pH 8.0
Pseudomonas putida
1.2.1.19
0.83
-
4-Aminobutyraldehyde
pH 9.5
Pseudomonas putida
1.2.1.54
0.03
-
4-guanidinobutyraldehyde
-
Pseudomonas putida
1.2.1.54
0.14
-
NAD+
-
Pseudomonas putida
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.19
57000
-
4 * 57000, SDS-PAGE
Pseudomonas putida
1.2.1.19
83000
-
gel filtration
Pseudomonas putida
1.2.1.19
240000
-
gel filtration
Pseudomonas putida
1.2.1.54
57000
-
2 * 57000, SDS-PAGE
Pseudomonas putida
1.2.1.54
107000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.2.1.19
4-aminobutanal + NAD+ + H2O
Pseudomonas putida
involved in the arginine decarboxylase pathway
4-aminobutanoate + NADH + H+
-
Pseudomonas putida
?
1.2.1.54
4-guanidinobutanal + NAD+
Pseudomonas putida
arginine catabolic pathway, involved in the degradation of arginine in Pseudomonas putida, cf. EC 1.2.1.19
4-guanidinobutanoate + NADH
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.19
using column chromatography on DEAE-cellulose, DEAE-Toyopearl, Toyopearl HW-55 and Affi-Gel Blue
Pseudomonas putida
1.2.1.54
-
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.19
additional information
-
-
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.2.1.19
3-aminopropanal + NAD+ + H2O
-
288069
Pseudomonas putida
3-aminopropanoate + NADH + H+
-
288069
Pseudomonas putida
?
1.2.1.19
4-aminobutanal + NAD+ + H2O
-
288069
Pseudomonas putida
4-aminobutanoate + NADH + H+
-
288069
Pseudomonas putida
?
1.2.1.19
4-aminobutanal + NAD+ + H2O
involved in the arginine decarboxylase pathway
288069
Pseudomonas putida
4-aminobutanoate + NADH + H+
-
288069
Pseudomonas putida
?
1.2.1.19
4-guanidinobutyraldehyde + NAD+ + H2O
-
288069
Pseudomonas putida
4-guanidinobutyrate + NADH + H+
-
288069
Pseudomonas putida
?
1.2.1.19
4-ureidobutyraldehyde + NAD+
-
288069
Pseudomonas putida
4-ureidobutyrate + NADH + H+
-
288069
Pseudomonas putida
?
1.2.1.19
5-aminovaleraldehyde + NAD+ + H2O
-
288069
Pseudomonas putida
5-aminovalerate + NADH + H+
-
288069
Pseudomonas putida
?
1.2.1.19
butyraldehyde + NAD+ + H2O
-
288069
Pseudomonas putida
butyrate + NADH + H+
-
288069
Pseudomonas putida
?
1.2.1.19
propionaldehyde + NAD+ + H2O
-
288069
Pseudomonas putida
propionate + NADH + H+
-
288069
Pseudomonas putida
?
1.2.1.19
valeraldehyde + NAD+ + H2O
-
288069
Pseudomonas putida
valerate + NADH + H+
-
288069
Pseudomonas putida
?
1.2.1.54
4-guanidinobutanal + NAD+
specific for
288069
Pseudomonas putida
4-guanidinobutanoate + NADH
-
-
-
?
1.2.1.54
4-guanidinobutanal + NAD+
arginine catabolic pathway, involved in the degradation of arginine in Pseudomonas putida, cf. EC 1.2.1.19
288069
Pseudomonas putida
4-guanidinobutanoate + NADH
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.1.19
tetramer
4 * 57000, SDS-PAGE
Pseudomonas putida
1.2.1.54
dimer
2 * 57000, SDS-PAGE
Pseudomonas putida
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.2.1.19
30
-
30 min, pH 5.0-8.0, stable; for 30 min, in 100 mM K-phosphate buffer, pH 7, allmost all activity retained; for 30 min, in 100 mM Na-acetate buffer, pH 5, allmost all activity retained; for 30 min, in 100 mM Na-bicine buffer, pH 8, allmost all activity retained
Pseudomonas putida
1.2.1.19
60
-
for 15 min, in 100 mM K-phosphate buffer, pH 7, 35% of activity lost; pH 7.0, 15 min, 35% loss of activity
Pseudomonas putida
1.2.1.54
40
-
15 min, pH 8.0, 70% loss of activity
Pseudomonas putida
1.2.1.54
50
-
15 min, pH 8.0, 100% loss of activity
Pseudomonas putida
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.19
8
-
optimum toward 4-aminobutyraldehyde
Pseudomonas putida
1.2.1.19
8.5
9.5
optimum toward 4-guanidinobutyraldehyde and 5-aminovaleraldehyde
Pseudomonas putida
1.2.1.54
9
-
-
Pseudomonas putida
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.1.19
6.5
9
4-aminobutyraldehyde, at pH 6.5: about 30% of activity maximum, at pH 9.0: about 70% of activity maximum
Pseudomonas putida
1.2.1.19
7
10
4-guanidinobutyraldehyde, at pH 7.0: about 35% of activity maximum, at pH 10.0: about 50% of activity maximum
Pseudomonas putida
1.2.1.54
7
10
pH 7.0: about 20% of activity maximum, pH 10.0: about 80% of activity maximum
Pseudomonas putida
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.2.1.19
7
-
most stable at neutral pH
Pseudomonas putida