BRENDA - Enzyme Database

Separation and properties of the NAD-linked and NADP-linked isozymes of succinic semialdehyde dehydrogenase in Euglena gracilis z

Tokunaga, M.; Nakano, Y.; Kitaoka, S.; Biochim. Biophys. Acta 429, 55-62 (1976)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.2.1.16
sulfhydryl reagents
beta-mercaptoethanol and dithiotreitol activate
Euglena gracilis
1.2.1.24
dithiothreitol
1 mM, 17% activation
Euglena gracilis
1.2.1.79
dithiothreitol
1 mM, 78% activation
Euglena gracilis
General Stability
EC Number
General Stability
Organism
1.2.1.16
ethyleneglycol, 30%, stabilizes
Euglena gracilis
1.2.1.16
glycerol, 30%, stabilizes
Euglena gracilis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.16
5,5'-dithiobis(2-nitrobenzoate)
-
Euglena gracilis
1.2.1.16
glyoxylate
-
Euglena gracilis
1.2.1.16
N-ethylmaleimide
-
Euglena gracilis
1.2.1.16
p-chloromercuribenzoate
-
Euglena gracilis
1.2.1.16
potassium phosphate buffer
-
Euglena gracilis
1.2.1.16
Zn2+
-
Euglena gracilis
1.2.1.24
5,5'-dithiobis(2-nitrobenzoic acid)
0.01 mM, 66% residual activity
Euglena gracilis
1.2.1.24
Ca2+
-
Euglena gracilis
1.2.1.24
Mg2+
-
Euglena gracilis
1.2.1.24
Mn2+
-
Euglena gracilis
1.2.1.24
N-ethylmaleimide
0.1 mM, complete loss of activity
Euglena gracilis
1.2.1.24
Ni2+
-
Euglena gracilis
1.2.1.24
succinate semialdehyde
substrate inhibition above 2 mM
Euglena gracilis
1.2.1.24
Zn2+
1 mM, 43% inhibition
Euglena gracilis
1.2.1.79
5,5'-dithiobis(2-nitrobenzoic acid)
0.01 mM, 29% residual activity
Euglena gracilis
1.2.1.79
Ca2+
-
Euglena gracilis
1.2.1.79
Mg2+
-
Euglena gracilis
1.2.1.79
Mn2+
-
Euglena gracilis
1.2.1.79
N-ethylmaleimide
0.1 mM, complete loss of activity
Euglena gracilis
1.2.1.79
Ni2+
-
Euglena gracilis
1.2.1.79
succinate semialdehyde
substrate inhibition above 1 mM
Euglena gracilis
1.2.1.79
Zn2+
1 mM, complete inhibition
Euglena gracilis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.16
0.038
-
NADP+
-
Euglena gracilis
1.2.1.16
1.06
-
succinate semialdehyde
-
Euglena gracilis
1.2.1.24
0.071
-
NAD+
pH 8.3, 35C
Euglena gracilis
1.2.1.24
0.16
-
succinate semialdehyde
pH 8.3, 35C
Euglena gracilis
1.2.1.79
0.038
-
NADP+
pH 8.7, 35C
Euglena gracilis
1.2.1.79
1.06
-
succinate semialdehyde
pH 8.7, 35C
Euglena gracilis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.1.16
K+
slight activation
Euglena gracilis
1.2.1.24
K+
5 mM, 84% activation
Euglena gracilis
1.2.1.79
K+
5 mM, 10% activation
Euglena gracilis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.16
150000
-
gel filtration
Euglena gracilis
1.2.1.24
150000
-
gel filtration
Euglena gracilis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.16
Euglena gracilis
-
-
-
1.2.1.24
Euglena gracilis
-
-
-
1.2.1.79
Euglena gracilis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.16
ammonium sulfate fractionation and DEAE-cellulose
Euglena gracilis
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.2.1.16
7.94
-
-
Euglena gracilis
1.2.1.24
3.25
-
pH 8.3, 35C
Euglena gracilis
1.2.1.79
7.9
-
pH 8.7, 35C
Euglena gracilis
Storage Stability
EC Number
Storage Stability
Organism
1.2.1.16
5C, complete inactivation after 2 days
Euglena gracilis
1.2.1.24
5C, unstable unless 30% glycerol is added
Euglena gracilis
1.2.1.79
5C, unstable unless 30% glycerol is added
Euglena gracilis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.16
malonic semialdehyde + NADP+ + H2O
hydrolyzed at 8.7% the rate of succinate semialdehyde
288042
Euglena gracilis
malonate + NADPH + H+
-
288042
Euglena gracilis
?
1.2.1.16
succinate semialdehyde + NADP+ + H2O
-
288042
Euglena gracilis
succinate + NADPH + H+
-
288042
Euglena gracilis
?
1.2.1.24
malonate semialdehyde + NAD+ + H2O
3.8% of the activity with succinate semialdehyde
288042
Euglena gracilis
malonate + NADH + 2 H+
-
-
-
?
1.2.1.24
additional information
no substrate: glyoxylic acid, formic acid, formaldehyde, acetaldehyde, glyoxal, furfural and acrolein
288042
Euglena gracilis
?
-
-
-
-
1.2.1.24
succinate semialdehyde + NAD+ + H2O
-
288042
Euglena gracilis
succinate + NADH + 2 H+
-
-
-
?
1.2.1.79
malonate semialdehyde + NADP+ + H2O
8.7% of the activity with succinate semialdehyde
288042
Euglena gracilis
malonate + NADPH + 2 H+
-
-
-
?
1.2.1.79
additional information
no substrate: glyoxylic acid, formic acid, formaldehyde, acetaldehyde, glyoxal, furfural and acrolein
288042
Euglena gracilis
?
-
-
-
-
1.2.1.79
succinate semialdehyde + NADP+ + H2O
-
288042
Euglena gracilis
succinate + NADPH + 2 H+
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.2.1.16
35
45
-
Euglena gracilis
1.2.1.24
35
45
-
Euglena gracilis
1.2.1.79
35
45
-
Euglena gracilis
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.2.1.16
60
-
up to, in presence of 30% glycerol
Euglena gracilis
1.2.1.24
60
-
stable up to
Euglena gracilis
1.2.1.79
60
-
stable up to
Euglena gracilis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.16
8.7
-
-
Euglena gracilis
1.2.1.24
8.7
-
-
Euglena gracilis
1.2.1.79
8.7
-
-
Euglena gracilis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.1.16
7.5
9.5
-
Euglena gracilis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.16
NADP+
specific for
Euglena gracilis
1.2.1.24
NAD+
-
Euglena gracilis
1.2.1.79
NADP+
-
Euglena gracilis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.2.1.16
sulfhydryl reagents
beta-mercaptoethanol and dithiotreitol activate
Euglena gracilis
1.2.1.24
dithiothreitol
1 mM, 17% activation
Euglena gracilis
1.2.1.79
dithiothreitol
1 mM, 78% activation
Euglena gracilis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.16
NADP+
specific for
Euglena gracilis
1.2.1.24
NAD+
-
Euglena gracilis
1.2.1.79
NADP+
-
Euglena gracilis
General Stability (protein specific)
EC Number
General Stability
Organism
1.2.1.16
ethyleneglycol, 30%, stabilizes
Euglena gracilis
1.2.1.16
glycerol, 30%, stabilizes
Euglena gracilis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.16
5,5'-dithiobis(2-nitrobenzoate)
-
Euglena gracilis
1.2.1.16
glyoxylate
-
Euglena gracilis
1.2.1.16
N-ethylmaleimide
-
Euglena gracilis
1.2.1.16
p-chloromercuribenzoate
-
Euglena gracilis
1.2.1.16
potassium phosphate buffer
-
Euglena gracilis
1.2.1.16
Zn2+
-
Euglena gracilis
1.2.1.24
5,5'-dithiobis(2-nitrobenzoic acid)
0.01 mM, 66% residual activity
Euglena gracilis
1.2.1.24
Ca2+
-
Euglena gracilis
1.2.1.24
Mg2+
-
Euglena gracilis
1.2.1.24
Mn2+
-
Euglena gracilis
1.2.1.24
N-ethylmaleimide
0.1 mM, complete loss of activity
Euglena gracilis
1.2.1.24
Ni2+
-
Euglena gracilis
1.2.1.24
succinate semialdehyde
substrate inhibition above 2 mM
Euglena gracilis
1.2.1.24
Zn2+
1 mM, 43% inhibition
Euglena gracilis
1.2.1.79
5,5'-dithiobis(2-nitrobenzoic acid)
0.01 mM, 29% residual activity
Euglena gracilis
1.2.1.79
Ca2+
-
Euglena gracilis
1.2.1.79
Mg2+
-
Euglena gracilis
1.2.1.79
Mn2+
-
Euglena gracilis
1.2.1.79
N-ethylmaleimide
0.1 mM, complete loss of activity
Euglena gracilis
1.2.1.79
Ni2+
-
Euglena gracilis
1.2.1.79
succinate semialdehyde
substrate inhibition above 1 mM
Euglena gracilis
1.2.1.79
Zn2+
1 mM, complete inhibition
Euglena gracilis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.16
0.038
-
NADP+
-
Euglena gracilis
1.2.1.16
1.06
-
succinate semialdehyde
-
Euglena gracilis
1.2.1.24
0.071
-
NAD+
pH 8.3, 35C
Euglena gracilis
1.2.1.24
0.16
-
succinate semialdehyde
pH 8.3, 35C
Euglena gracilis
1.2.1.79
0.038
-
NADP+
pH 8.7, 35C
Euglena gracilis
1.2.1.79
1.06
-
succinate semialdehyde
pH 8.7, 35C
Euglena gracilis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.1.16
K+
slight activation
Euglena gracilis
1.2.1.24
K+
5 mM, 84% activation
Euglena gracilis
1.2.1.79
K+
5 mM, 10% activation
Euglena gracilis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.16
150000
-
gel filtration
Euglena gracilis
1.2.1.24
150000
-
gel filtration
Euglena gracilis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.16
ammonium sulfate fractionation and DEAE-cellulose
Euglena gracilis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1.2.1.16
7.94
-
-
Euglena gracilis
1.2.1.24
3.25
-
pH 8.3, 35C
Euglena gracilis
1.2.1.79
7.9
-
pH 8.7, 35C
Euglena gracilis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.2.1.16
5C, complete inactivation after 2 days
Euglena gracilis
1.2.1.24
5C, unstable unless 30% glycerol is added
Euglena gracilis
1.2.1.79
5C, unstable unless 30% glycerol is added
Euglena gracilis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.16
malonic semialdehyde + NADP+ + H2O
hydrolyzed at 8.7% the rate of succinate semialdehyde
288042
Euglena gracilis
malonate + NADPH + H+
-
288042
Euglena gracilis
?
1.2.1.16
succinate semialdehyde + NADP+ + H2O
-
288042
Euglena gracilis
succinate + NADPH + H+
-
288042
Euglena gracilis
?
1.2.1.24
malonate semialdehyde + NAD+ + H2O
3.8% of the activity with succinate semialdehyde
288042
Euglena gracilis
malonate + NADH + 2 H+
-
-
-
?
1.2.1.24
additional information
no substrate: glyoxylic acid, formic acid, formaldehyde, acetaldehyde, glyoxal, furfural and acrolein
288042
Euglena gracilis
?
-
-
-
-
1.2.1.24
succinate semialdehyde + NAD+ + H2O
-
288042
Euglena gracilis
succinate + NADH + 2 H+
-
-
-
?
1.2.1.79
malonate semialdehyde + NADP+ + H2O
8.7% of the activity with succinate semialdehyde
288042
Euglena gracilis
malonate + NADPH + 2 H+
-
-
-
?
1.2.1.79
additional information
no substrate: glyoxylic acid, formic acid, formaldehyde, acetaldehyde, glyoxal, furfural and acrolein
288042
Euglena gracilis
?
-
-
-
-
1.2.1.79
succinate semialdehyde + NADP+ + H2O
-
288042
Euglena gracilis
succinate + NADPH + 2 H+
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.2.1.16
35
45
-
Euglena gracilis
1.2.1.24
35
45
-
Euglena gracilis
1.2.1.79
35
45
-
Euglena gracilis
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.2.1.16
60
-
up to, in presence of 30% glycerol
Euglena gracilis
1.2.1.24
60
-
stable up to
Euglena gracilis
1.2.1.79
60
-
stable up to
Euglena gracilis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.16
8.7
-
-
Euglena gracilis
1.2.1.24
8.7
-
-
Euglena gracilis
1.2.1.79
8.7
-
-
Euglena gracilis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.1.16
7.5
9.5
-
Euglena gracilis
Expression
EC Number
Organism
Commentary
Expression
1.2.1.24
Euglena gracilis
induction by growth on glutamate
up
1.2.1.79
Euglena gracilis
induction by growth on glutamate
up
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.2.1.24
Euglena gracilis
induction by growth on glutamate
up
1.2.1.79
Euglena gracilis
induction by growth on glutamate
up