EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.13 | iodoacetate | preincubation with glyceraldehyde-phosphate, 10fold molar excess over iodoacetate, prevents inactivation | Spinacia oleracea |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.13 | additional information | - |
additional information | - |
Spinacia oleracea |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.2.1.13 | chloroplast | - |
Spinacia oleracea | 9507 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.13 | 37000 | - |
x * 43000 + x * 37000, SDS-PAGE | Spinacia oleracea |
1.2.1.13 | 43000 | - |
x * 43000 + x * 37000, SDS-PAGE | Spinacia oleracea |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.13 | Spinacia oleracea | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.13 | - |
Spinacia oleracea |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.13 | 13.92 | - |
NAD+-dependent activity | Spinacia oleracea |
1.2.1.13 | 15.4 | - |
NADP+-dependent activity | Spinacia oleracea |
EC Number | Storage Stability | Organism |
---|---|---|
1.2.1.13 | 4°C, pH 8.5, protein concentration 2.5 mg/ml, apoprotein, complete loss of activity after 48 h | Spinacia oleracea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.13 | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Spinacia oleracea | 3-phospho-D-glyceroyl phosphate + NADH | - |
r | |
1.2.1.13 | D-glyceraldehyde 3-phosphate + phosphate + NADP+ | - |
Spinacia oleracea | 3-phospho-D-glyceroyl phosphate + NADPH | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.13 | ? | x * 43000 + x * 37000, SDS-PAGE | Spinacia oleracea |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.13 | 8 | 8.8 | both directions | Spinacia oleracea |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.13 | NADP+ | cofactor | Spinacia oleracea |