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Literature summary extracted from

  • Heinz, F.; Kulbe, K.D.
    Glyceraldehydephosphate dehydrogenase from liver. I. Isolation and characterization of the bovine liver enzyme (1970), Hoppe-Seyler's Z. Physiol. Chem., 351, 249-262.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.12 2,3-Dimercaptopropanol poor activation Bos taurus
1.2.1.12 2-mercaptoethanol 10 mM, 2fold activation of the oxidation of D-glyceraldehyde 3-phosphate Bos taurus
1.2.1.12 Cys 10 mM, 2.8fold activation of the oxidation of D-glyceraldehyde 3-phosphate Bos taurus
1.2.1.12 dithioerythritol 10 mM, 2fold activation of the oxidation of D-glyceraldehyde 3-phosphate Bos taurus
1.2.1.12 EDTA poor activation Bos taurus
1.2.1.12 GSH poor activation Bos taurus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.12 crystalline enzyme does not contain NAD+ Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.12 PCMB
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.12 0.031
-
 NAD+ pH 8.9 Bos taurus
1.2.1.12 0.19
-
 NAD+ pH 8.0 Bos taurus
1.2.1.12 0.24
-
 D-glyceraldehyde 3-phosphate pH 8.0 Bos taurus
1.2.1.12 0.385
-
 D-glyceraldehyde 3-phosphate pH 8.9 Bos taurus
1.2.1.12 1.54
-
 arsenate pH 8.9 Bos taurus
1.2.1.12 1.7
-
 arsenate pH 8.0 Bos taurus
1.2.1.12 2.9
-
 D-glyceraldehyde 3-phosphate pH 8.0 Bos taurus
1.2.1.12 3.4
-
 phosphate pH 8.9 Bos taurus
1.2.1.12 7.15
-
 phosphate pH 8.0 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.12 142000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.12
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.12 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.12 50.5
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 3-phospho-D-glyceroyl phosphate + NADH
-
 Bos taurus D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 r
1.2.1.12 D-glyceraldehyde 3-phosphate + arsenate + NAD+
-
 Bos taurus 3-phospho-D-glyceroyl arsenate + NADH
-
 ?
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Bos taurus 3-phospho-D-glyceroyl phosphate + NADH
-
 r
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Bos taurus 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.12 7
-
 reduction of 3-phospho-D-glyceroyl phosphate, triethanolamine buffer Bos taurus
1.2.1.12 7.4 8.1 reduction of 1,3-diphosphogycerate, Tris buffer Bos taurus
1.2.1.12 7.8 8.1 oxidation of D-glyceraldehyde 3-phosphate, Tris buffer and triethanolamine buffer Bos taurus
1.2.1.12 8.5
-
 oxidation of D-glyceraldehyde 3-phosphate, glycine buffer Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NAD+
-
 Bos taurus
1.2.1.12 NADH cofactor Bos taurus