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Literature summary extracted from
- Purification and properties of D-glyceraldehyde-3-phosphate dehydrogenase from an extreme thermophile, Thermus thermophilus strain HB 8 (1976), Eur. J. Biochem., 64, 57-68.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.1.12 | ethanol | 30% v/v, 5fold activation | Thermus thermophilus |  |
| 1.2.1.12 | propan-1-ol | activates | Thermus thermophilus | |
| 1.2.1.12 | propan-2-ol | activates | Thermus thermophilus | |
| 1.2.1.12 | tetrabutylammonium chloride | 50% activation | Thermus thermophilus | |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.2.1.12 | - |
Thermus thermophilus |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.2.1.12 | 8 M urea, 40% inactivation | Thermus thermophilus |
| 1.2.1.12 | repeated freezing and thawing brings about 10-20% inactivation | Thermus thermophilus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.1.12 | CdCl2 | - |
Thermus thermophilus | |
| 1.2.1.12 | Cu2+ | - |
Thermus thermophilus | |
| 1.2.1.12 | HgCl2 | - |
Thermus thermophilus | |
| 1.2.1.12 | iodoacetamide | 0.5 mM, 9% inhibition | Thermus thermophilus | |
| 1.2.1.12 | iodoacetate | 0.2 mM, 67% inhibition | Thermus thermophilus | |
| 1.2.1.12 | Na2S4O6 | 0.1 mM, complete inhibition | Thermus thermophilus | |
| 1.2.1.12 | NiCl2 | - |
Thermus thermophilus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.1.12 | 0.1 | - |
NAD+ | - |
Thermus thermophilus | |
| 1.2.1.12 | 0.3 | - |
D-glyceraldehyde 3-phosphate | - |
Thermus thermophilus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.1.12 | (NH4)2SO4 | 0.045 M, 10fold activation | Thermus thermophilus | |
| 1.2.1.12 | CsCl | activates | Thermus thermophilus | |
| 1.2.1.12 | CsCl | maximal activation: 20fold | Thermus thermophilus | |
| 1.2.1.12 | K+ | maximal activation: 22fold | Thermus thermophilus | |
| 1.2.1.12 | LiCl | maximal activation: 10fold | Thermus thermophilus | |
| 1.2.1.12 | Na2SO4 | up to 5fold activation | Thermus thermophilus | |
| 1.2.1.12 | NH4Cl | 0.1 M, 25fold activation | Thermus thermophilus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.12 | 33700 | - |
4 * 33700, SDS-PAGE | Thermus thermophilus |
| 1.2.1.12 | 128000 | - |
equilibrium sedimentation | Thermus thermophilus |
| 1.2.1.12 | 135000 | - |
gel filtration | Thermus thermophilus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.1.12 | Thermus thermophilus | - |
- |
- |
| 1.2.1.12 | Thermus thermophilus HB 8 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.2.1.12 | - |
Thermus thermophilus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.12 | 5.4 | - |
- |
Thermus thermophilus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.2.1.12 | -20°C, stable for at least 2 years | Thermus thermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.12 | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Thermus thermophilus | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
| 1.2.1.12 | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Thermus thermophilus HB 8 | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.2.1.12 | tetramer | 4 * 33700, SDS-PAGE | Thermus thermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.12 | 85 | - |
pH 8.5, protein concentration 2.1 mg/ml, 10 min, stable | Thermus thermophilus |
| 1.2.1.12 | 90 | - |
pH 8.5, protein concentration 2.1 mg/ml, 10 min, about 20% loss of acticity | Thermus thermophilus |
| 1.2.1.12 | 95 | - |
pH 8.5, protein concentration 2.1 mg/ml, 10 min, about 85% loss of acticity | Thermus thermophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.12 | 8.3 | - |
- |
Thermus thermophilus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.12 | 7 | 8.8 | pH 7.0: about 55% of maximal activity, pH 8.8: about 95% of maximal activity | Thermus thermophilus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.1.12 | NAD+ | - |
Thermus thermophilus | |
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