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Literature summary extracted from

  • Ryzlak, M.T.; Pietruszko, R.
    Heterogeneity of glyceraldehyde-3-phosphate dehydrogenase from human brain (1988), Biochim. Biophys. Acta, 954, 309-324.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.12 0.002
-
 3-phospho-D-glyceroyl phosphate enzyme form E6.8, pH 7 Homo sapiens
1.2.1.12 0.01
-
 NAD+ enzyme form E8.5, pH 9 Homo sapiens
1.2.1.12 0.01
-
 1,3-diphosphoglyceric acid enzyme form E6.6, pH 7 Homo sapiens
1.2.1.12 0.012
-
 1,3-diphosphoglyceric acid enzyme form E8.5, pH 7 Homo sapiens
1.2.1.12 0.018
-
 1,3-diphosphoglyceric acid enzyme form E9.0, pH 7 Homo sapiens
1.2.1.12 0.02
-
 NAD+ enzyme form E9.0, pH 9 Homo sapiens
1.2.1.12 0.027
-
 NAD+ enzyme form E6.8, pH 9 Homo sapiens
1.2.1.12 0.032
-
 3-phospho-D-glyceroyl phosphate enzyme form E9.0, pH 9 Homo sapiens
1.2.1.12 0.035
-
 3-phospho-D-glyceroyl phosphate enzyme form E8.5, pH 9 Homo sapiens
1.2.1.12 0.042
-
 3-phospho-D-glyceroyl phosphate enzyme form E6.6, pH 9 Homo sapiens
1.2.1.12 0.1
-
 DL-glyceraldehyde enzyme form E8.5, pH 7 Homo sapiens
1.2.1.12 0.119
-
 erythrose 4-phosphate enzyme form E8.5, pH 7 Homo sapiens
1.2.1.12 0.127
-
 erythrose 4-phosphate enzyme form E9.0, pH 7 Homo sapiens
1.2.1.12 0.143
-
 NAD+ enzyme form E6.6, pH 9 Homo sapiens
1.2.1.12 0.172
-
 3-phospho-D-glyceroyl phosphate enzyme form E6.8, pH 9 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.12 mitochondrion
-
 Rattus norvegicus 5739
-
1.2.1.12 mitochondrion enzyme form E6.8, E8.5 and E9.0 Homo sapiens 5739
-
1.2.1.12 additional information the subcellular localization of enzyme form E6.6 is uncertain Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.12 Zn enzyme form E8.5 contains 0.64 gatoms of zinc per mol of enzyme, enzyme form E9.0 contain 2.76 gatom of zinc per mol of enzyme Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.12 29500
-
 4 * 29500, enzyme form E8.5, SDS-PAGE Homo sapiens
1.2.1.12 33000
-
 4 * 33000, enzyme form E9.5, SDS-PAGE Homo sapiens
1.2.1.12 36000
-
 x * 36000 + x * 38000, enzyme form E6.8, SDS-PAGE Homo sapiens
1.2.1.12 38000
-
 x * 36000 + x * 38000, enzyme form E6.8, SDS-PAGE Homo sapiens
1.2.1.12 58000
-
 1 * 58000 + 1 * 61000, enzyme form E6.6, SDS-PAGE Homo sapiens
1.2.1.12 61000
-
 1 * 58000 + 1 * 61000, enzyme form E6.6, SDS-PAGE Homo sapiens
1.2.1.12 98000
-
 enzyme form E6.6, gel filtration Homo sapiens
1.2.1.12 120000
-
 enzyme form E8.5, gel filtration Homo sapiens
1.2.1.12 133000
-
 enzyme form E9.0, gel filtration Homo sapiens
1.2.1.12 142000
-
 enzyme form E6.8, gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Homo sapiens
-
-
-
1.2.1.12 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.12 4 enzyme forms: E6.6, E6.8, E8.5 and E9.0 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.12 brain
-
 Homo sapiens
-
1.2.1.12 liver
-
 Homo sapiens
-
1.2.1.12 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.12 8.6
-
 enzyme form E6.6 Homo sapiens
1.2.1.12 13
-
 enzyme form E6.8 Homo sapiens
1.2.1.12 158
-
 enzyme form E8.5 Homo sapiens
1.2.1.12 620
-
 enzyme form E9.0 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 3-phospho-D-glyceroyl phosphate + NADH
-
 Homo sapiens D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 r
1.2.1.12 acetaldehyde + phosphate + NAD+ enzyme form E6.6 shows 27% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E6.8 shows 9% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 6% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.4% of the activity with D-glyceraldehyde 3-phosphate Homo sapiens acetyl phosphate + NADH
-
 ?
1.2.1.12 butyraldehyde + phosphate + NAD+ enzyme form E6.6 shows 10% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E6.8 shows 15% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 12% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.9% of the activity with D-glyceraldehyde 3-phosphate Homo sapiens butyryl phosphate + NADH
-
 ?
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Homo sapiens 3-phospho-D-glyceroyl phosphate + NADH
-
 r
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Rattus norvegicus 3-phospho-D-glyceroyl phosphate + NADH
-
 ?
1.2.1.12 DL-glyceraldehyde + phosphate + NAD+ enzyme form E6.6 shows no activity, enzyme form E6.8 shows 2.5% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 30% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 3.0% of the activity with D-glyceraldehyde 3-phosphate Homo sapiens D-glyceroyl phosphate + NADH
-
 ?
1.2.1.12 erythrose 4-phosphate + phosphate + NAD+ enzyme form E6.6 shows no activity, enzyme form E6.8 shows 1.2% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 25% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 1.5% of the activity with D-glyceraldehyde 3-phosphate Homo sapiens ? + NADH
-
 ?
1.2.1.12 glucose + phosphate + NAD+ enzyme form E6.6 shows no activity, enzyme form E6.8 shows 0.6% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 6.0% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.8% of the activity with D-glyceraldehyde 3-phosphate Homo sapiens ? + NADH
-
 ?
1.2.1.12 propionaldehyde + phosphate + NAD+ enzyme form E6.6 shows 33% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E6.8 shows 12% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 10% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.8% of the activity with D-glyceraldehyde 3-phosphate Homo sapiens propionyl phosphate + NADH
-
 ?
1.2.1.12 valeraldehyde + phosphate + NAD+ enzyme form E6.6 shows no activity, enzyme form E6.8 shows 19% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 18% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.9% of the activity with D-glyceraldehyde 3-phosphate Homo sapiens pentanoyl phosphate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.12 dimer 1 * 58000 + 1 * 61000, enzyme form E6.6, SDS-PAGE Homo sapiens
1.2.1.12 tetramer 4 * 29500, enzyme form E8.5, SDS-PAGE Homo sapiens
1.2.1.12 tetramer x * 36000 + x * 38000, enzyme form E6.8, SDS-PAGE Homo sapiens
1.2.1.12 tetramer 4 * 33000, enzyme form E9.5, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.12 7
-
 enzyme form E6.8, two pH optima: pH 7.0 and pH 8.5, with activity between pH 7.5 and pH 8.0 being rather low Homo sapiens
1.2.1.12 8.5
-
 enzyme form E6.8, two pH optima: pH 7.0 and pH 8.5, with activity between pH 7.5 and pH 8.0 being rather low Homo sapiens
1.2.1.12 9.8
-
 enzyme form E8.5, D-glyceraldehyde 3-phosphate Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NAD+
-
 Homo sapiens
1.2.1.12 NAD+
-
 Rattus norvegicus
1.2.1.12 NADH cofactor Homo sapiens