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Literature summary extracted from

  • Kato, N.; Miyawaki, N.; Sakazawa, C.
    Formaldehyde dehydrogenase from formaldehyde-resistant Debaryomyces vanriji FT-1 and Pseudomonas putida F61 (1983), Agric. Biol. Chem., 47, 415-416.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.284 0.15
-
 NAD+
-
 Pseudomonas putida
1.1.1.284 0.31
-
 NAD+
-
 Schwanniomyces vanrijiae
1.1.1.284 0.39
-
 formaldehyde
-
 Pseudomonas putida
1.1.1.284 1.14
-
 formaldehyde
-
 Schwanniomyces vanrijiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.284 40000
-
 2 * 40000, SDS-PAGE Schwanniomyces vanrijiae
1.1.1.284 42000
-
 2 * 42000, SDS-PAGE Pseudomonas putida
1.1.1.284 72000
-
 gel filtration Pseudomonas putida
1.1.1.284 72000
-
 gel filtration Schwanniomyces vanrijiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.284 Pseudomonas putida
-
-
-
1.1.1.284 Pseudomonas putida F61
-
-
-
1.1.1.284 Schwanniomyces vanrijiae
-
-
-
1.1.1.284 Schwanniomyces vanrijiae FT-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.284
-
 Pseudomonas putida
1.1.1.284
-
 Schwanniomyces vanrijiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.284 184
-
-
 Schwanniomyces vanrijiae
1.1.1.284 185
-
-
 Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.1.1.284 5°C, 10 mM potassium phosphate buffer, pH 7.5, 0.1% 2-mercaptoethanol, stable for at least 1 month Pseudomonas putida
1.1.1.284 5°C, 10 mM potassium phosphate buffer, pH 7.5, 0.1% 2-mercaptoethanol, stable for at least 1 month Schwanniomyces vanrijiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.284 formaldehyde + glutathione + NAD+
-
 Pseudomonas putida S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 formaldehyde + glutathione + NAD+
-
 Schwanniomyces vanrijiae S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 formaldehyde + glutathione + NAD+
-
 Pseudomonas putida F61 S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 formaldehyde + glutathione + NAD+
-
 Schwanniomyces vanrijiae FT-1 S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 methylglyoxal + glutathione + NAD+
-
 Pseudomonas putida S-pyruvylglutathione + NADH
-
 ?
1.1.1.284 methylglyoxal + glutathione + NAD+
-
 Schwanniomyces vanrijiae S-pyruvylglutathione + NADH
-
 ?
1.1.1.284 methylglyoxal + glutathione + NAD+
-
 Pseudomonas putida F61 S-pyruvylglutathione + NADH
-
 ?
1.1.1.284 methylglyoxal + glutathione + NAD+
-
 Schwanniomyces vanrijiae FT-1 S-pyruvylglutathione + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.284 dimer 2 * 40000, SDS-PAGE Schwanniomyces vanrijiae
1.1.1.284 dimer 2 * 42000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.284 35
-
-
 Schwanniomyces vanrijiae
1.1.1.284 45
-
-
 Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.284 30
-
 10 min, stable Schwanniomyces vanrijiae
1.1.1.284 40
-
 10 min, stable Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.284 8
-
-
 Schwanniomyces vanrijiae
1.1.1.284 9
-
-
 Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.284 6 8.5 4°C, 3 d, stable Pseudomonas putida
1.1.1.284 6.5 8.5 4°C, 3 d, stable Schwanniomyces vanrijiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.284 NAD+
-
 Pseudomonas putida
1.1.1.284 NAD+
-
 Schwanniomyces vanrijiae