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Literature summary extracted from

  • Uotila, L.; Koivusalo, M.
    Formaldehyde dehydrogenase (1981), Methods Enzymol., 77, 314-320.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.284 NAD+ and NADH protect from denaturation by high temperatures, acid pH and storage at 0°C Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.284 ADP-ribose
-
 Homo sapiens
1.1.1.284 Alpha-NAD+
-
 Homo sapiens
1.1.1.284 Hg2+
-
 Homo sapiens
1.1.1.284 NAD+ competitive, nonlinear inhibitor, when the concentration of NADH varies at constant S-formylglutathione concentration, noncompetitive inhibitor when the concentration of S-formylglutathione is varied at constant NADH concentration Homo sapiens
1.1.1.284 p-hydroxymercuribenzoate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.284 0.0042
-
 NADH pH 5.7 Homo sapiens
1.1.1.284 0.008
-
 glutathione pH 8 Homo sapiens
1.1.1.284 0.009
-
 formaldehyde pH 8 Homo sapiens
1.1.1.284 0.045
-
 NADPH pH 5.7 Homo sapiens
1.1.1.284 0.3
-
 S-formylglutathione pH 5.7, with NADH as cofactor Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.284 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.284 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.284 3.17
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.1.1.284 0°C, half-life in absence of a stabilizer is a few h, in presence of 1 mM NAD+ or 40% v/v glycerol the half life is about 1 month, 0.5 mM NADH is even more effective Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.284 3-ethoxy-2-hydroxybutyraldehyde + glutathione + NAD+ weak activity Homo sapiens S-(3-ethoxy-2-hydroxybutyryl)-glutathione + NADH + H+
-
 ?
1.1.1.284 formaldehyde + glutathione + NAD+ at pH 8 the rate of the reverse reaction with S-formylglutathione and NADH is about the same as that of the forward reaction with formaldehyde, glutathione and NAD+. At pH 5.7 the rate of the reverse reaction with S-formylglutathione and NADH is 3.9times and with S-formylglutathione and NADPH 2.0times, that of the forward reaction rate with NAD+ at pH 8.0 Homo sapiens S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 formaldehyde + glutathione + NAD+ at pH 8 the rate of the reverse reaction with S-formylglutathione and NADH is about the same as that of the forward reaction with formaldehyde, glutathione and NAD+. At pH 5.7 the rate of the reverse reaction with S-formylglutathione and NADH is 3.9times and with S-formylglutathione and NADPH 2.0times, that of the forward reaction rate with NAD+ at pH 8.0 Homo sapiens S-formylglutathione + NADH + H+
-
 r
1.1.1.284 formaldehyde + glutathione + NADP+
-
 Homo sapiens S-formylglutathione + NADPH + H+
-
 r
1.1.1.284 glyoxal + glutathione + NAD+ weak activity Homo sapiens S-oxoacetylglutathione + NADH
-
 ?
1.1.1.284 hydroxypyruvaldehyde + glutathione + NAD+ weak activity Homo sapiens S-hydroxypyruvylglutathione + NADH
-
 ?
1.1.1.284 methylglyoxal + glutathione + NAD+ 85% of the activity with formaldehyde Homo sapiens S-pyruvylglutathione + NADH
-
 ?
1.1.1.284 S-formylglutathione + NADH
-
 Homo sapiens formaldehyde + glutathione + NAD+
-
 r
1.1.1.284 S-formylglutathione + NADPH
-
 Homo sapiens formaldehyde + glutathione + NADP+
-
 r
1.1.1.284 S-pyruvylglutathione + NADH
-
 Homo sapiens methylglyoxal + glutathione + NAD+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.284 5.7
-
 reaction with S-formylglutathione and NADH Homo sapiens
1.1.1.284 8
-
 oxidation of formaldehyde Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.284 NAD+
-
 Homo sapiens
1.1.1.284 NADH
-
 Homo sapiens
1.1.1.284 NADP+ at pH 8 the Km-value is about 1000times greater than that for NAD+. At pH 6 NADP+ is used better but this reaction appears to be without physiological significance Homo sapiens
1.1.1.284 NADPH
-
 Homo sapiens