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Literature summary extracted from

  • Schuette, H.; Kula, M.R.; Sahm, H.
    Formaldehyde dehydrogenase from Candida boidinii (1982), Methods Enzymol., 89, 527-531.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.284 ADP
-
 [Candida] boidinii
1.1.1.284 AMP
-
 [Candida] boidinii
1.1.1.284 ATP
-
 [Candida] boidinii
1.1.1.284 Cd2+
-
 [Candida] boidinii
1.1.1.284 Cu2+
-
 [Candida] boidinii
1.1.1.284 Hg2+
-
 [Candida] boidinii
1.1.1.284 PCMB
-
 [Candida] boidinii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.284 0.09
-
 NAD+
-
 [Candida] boidinii
1.1.1.284 0.13
-
 glutathione
-
 [Candida] boidinii
1.1.1.284 0.25
-
 formaldehyde
-
 [Candida] boidinii
1.1.1.284 1.2
-
 methylglyoxal
-
 [Candida] boidinii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.284 40000
-
 2 * 40000, SDS-PAGE [Candida] boidinii
1.1.1.284 80000
-
 equilibrium sedimentation [Candida] boidinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.284 formaldehyde + glutathione + NAD+ [Candida] boidinii inducible enzyme S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 formaldehyde + glutathione + NAD+ [Candida] boidinii key enzyme of the dissimilatory pathway of the methanol metabolism S-formylglutathione + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.284 [Candida] boidinii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.284
-
 [Candida] boidinii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.284 47.9
-
-
 [Candida] boidinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.284 formaldehyde + glutathione + NAD+
-
 [Candida] boidinii S-formylglutathione + NADH + H+
-
 r
1.1.1.284 formaldehyde + glutathione + NAD+ inducible enzyme [Candida] boidinii S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 formaldehyde + glutathione + NAD+ key enzyme of the dissimilatory pathway of the methanol metabolism [Candida] boidinii S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 methylglyoxal + glutathione + NAD+
-
 [Candida] boidinii S-pyruvylglutathione + NADH
-
 ?
1.1.1.284 S-formylglutathione + NADH
-
 [Candida] boidinii formaldehyde + glutathione + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.284 dimer 2 * 40000, SDS-PAGE [Candida] boidinii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.284 34
-
-
 [Candida] boidinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.284 8.5
-
-
 [Candida] boidinii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.284 NAD+
-
 [Candida] boidinii
1.1.1.284 NADH
-
 [Candida] boidinii
1.1.1.284 NADP+ no activity with NADP+ [Candida] boidinii