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Literature summary extracted from

  • Krafft, T.; Macy, J.M.
    Purification and characterization of the respiratory arsenate reductase of Chrysiogenes arsenatis (1998), Eur. J. Biochem., 255, 647-653.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.20.9.1 0.3
-
 arsenate
-
 Chrysiogenes arsenatis
1.20.99.1 0.3
-
 arsenate
-
 Chrysiogenes arsenatis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.20.9.1 periplasm
-
 Chrysiogenes arsenatis
-
-
1.20.99.1 periplasm
-
 Chrysiogenes arsenatis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.20.9.1 Fe contains Chrysiogenes arsenatis
1.20.9.1 Mo contains Chrysiogenes arsenatis
1.20.9.1 Zn contains Chrysiogenes arsenatis
1.20.99.1 Iron contains several iron-sulfur centres as prosthetic groups Chrysiogenes arsenatis
1.20.99.1 Zinc it is possible that one zinc atom represents an additional cofactor Chrysiogenes arsenatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.20.9.1 29000
-
 1 * 87000 + 1 * 29000, SDS-PAGE Chrysiogenes arsenatis
1.20.9.1 87000
-
 1 * 87000 + 1 * 29000, SDS-PAGE Chrysiogenes arsenatis
1.20.9.1 123000
-
 gel filtration Chrysiogenes arsenatis
1.20.99.1 29000
-
 alpha,beta, 1 * 87000, ArrA, + 1 * 29000, ArrB, SDS-PAGE Chrysiogenes arsenatis
1.20.99.1 87000
-
 alpha,beta, 1 * 87000, ArrA, + 1 * 29000, ArrB, SDS-PAGE Chrysiogenes arsenatis
1.20.99.1 123000
-
 gel filtration Chrysiogenes arsenatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.20.99.1 arsenate + acetate Chrysiogenes arsenatis anaerobic respiration using arsenate as terminal electron acceptor and acetate as the electron donor arsenite + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.20.9.1 Chrysiogenes arsenatis
-
-
-
1.20.99.1 Chrysiogenes arsenatis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.20.9.1
-
 Chrysiogenes arsenatis
1.20.99.1
-
 Chrysiogenes arsenatis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.20.9.1 7013
-
-
 Chrysiogenes arsenatis
1.20.99.1 116.1
-
-
 Chrysiogenes arsenatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.20.9.1 arsenate + azurin(red)
-
 Chrysiogenes arsenatis arsenite + H2O + azurin(ox)
-
 ?
1.20.99.1 arsenate + acetate specific for arsenate Chrysiogenes arsenatis arsenite + ?
-
 ?
1.20.99.1 arsenate + acetate anaerobic respiration using arsenate as terminal electron acceptor and acetate as the electron donor Chrysiogenes arsenatis arsenite + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.20.9.1 dimer 1 * 87000 + 1 * 29000, SDS-PAGE Chrysiogenes arsenatis
1.20.99.1 dimer alpha,beta, 1 * 87000, ArrA, + 1 * 29000, ArrB, SDS-PAGE Chrysiogenes arsenatis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.20.9.1 14400
-
 arsenate
-
 Chrysiogenes arsenatis
1.20.99.1 14400
-
 arsenate
-
 Chrysiogenes arsenatis

Cofactor

EC Number Cofactor Comment Organism Structure
1.20.99.1 Molybdenum cofactor Chrysiogenes arsenatis