BRENDA - Enzyme Database show

Purification and characterization of the respiratory arsenate reductase of Chrysiogenes arsenatis

Krafft, T.; Macy, J.M.; Eur. J. Biochem. 255, 647-653 (1998)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.9.1
0.3
-
arsenate
-
Chrysiogenes arsenatis
1.20.99.1
0.3
-
arsenate
-
Chrysiogenes arsenatis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.20.9.1
periplasm
-
Chrysiogenes arsenatis
-
-
1.20.99.1
periplasm
-
Chrysiogenes arsenatis
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.20.9.1
Fe
contains
Chrysiogenes arsenatis
1.20.9.1
Mo
contains
Chrysiogenes arsenatis
1.20.9.1
Zn
contains
Chrysiogenes arsenatis
1.20.99.1
Iron
contains several iron-sulfur centres as prosthetic groups
Chrysiogenes arsenatis
1.20.99.1
Zinc
it is possible that one zinc atom represents an additional cofactor
Chrysiogenes arsenatis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.20.9.1
29000
-
1 * 87000 + 1 * 29000, SDS-PAGE
Chrysiogenes arsenatis
1.20.9.1
87000
-
1 * 87000 + 1 * 29000, SDS-PAGE
Chrysiogenes arsenatis
1.20.9.1
123000
-
gel filtration
Chrysiogenes arsenatis
1.20.99.1
29000
-
alpha,beta, 1 * 87000, ArrA, + 1 * 29000, ArrB, SDS-PAGE
Chrysiogenes arsenatis
1.20.99.1
87000
-
alpha,beta, 1 * 87000, ArrA, + 1 * 29000, ArrB, SDS-PAGE
Chrysiogenes arsenatis
1.20.99.1
123000
-
gel filtration
Chrysiogenes arsenatis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.20.99.1
arsenate + acetate
Chrysiogenes arsenatis
anaerobic respiration using arsenate as terminal electron acceptor and acetate as the electron donor
arsenite + ?
-
Chrysiogenes arsenatis
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.20.9.1
Chrysiogenes arsenatis
-
-
-
1.20.99.1
Chrysiogenes arsenatis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.20.9.1
-
Chrysiogenes arsenatis
1.20.99.1
-
Chrysiogenes arsenatis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.20.9.1
7013
-
-
Chrysiogenes arsenatis
1.20.99.1
116.1
-
-
Chrysiogenes arsenatis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.9.1
arsenate + azurin(red)
-
287859
Chrysiogenes arsenatis
arsenite + H2O + azurin(ox)
-
-
-
?
1.20.99.1
arsenate + acetate
specific for arsenate
287859
Chrysiogenes arsenatis
arsenite + ?
-
287859
Chrysiogenes arsenatis
?
1.20.99.1
arsenate + acetate
anaerobic respiration using arsenate as terminal electron acceptor and acetate as the electron donor
287859
Chrysiogenes arsenatis
arsenite + ?
-
287859
Chrysiogenes arsenatis
?
Subunits
EC Number
Subunits
Commentary
Organism
1.20.9.1
dimer
1 * 87000 + 1 * 29000, SDS-PAGE
Chrysiogenes arsenatis
1.20.99.1
dimer
alpha,beta, 1 * 87000, ArrA, + 1 * 29000, ArrB, SDS-PAGE
Chrysiogenes arsenatis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.20.9.1
14400
-
arsenate
-
Chrysiogenes arsenatis
1.20.99.1
14400
-
arsenate
-
Chrysiogenes arsenatis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.20.99.1
Molybdenum
cofactor
Chrysiogenes arsenatis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.20.99.1
Molybdenum
cofactor
Chrysiogenes arsenatis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.9.1
0.3
-
arsenate
-
Chrysiogenes arsenatis
1.20.99.1
0.3
-
arsenate
-
Chrysiogenes arsenatis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.20.9.1
periplasm
-
Chrysiogenes arsenatis
-
-
1.20.99.1
periplasm
-
Chrysiogenes arsenatis
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.20.9.1
Fe
contains
Chrysiogenes arsenatis
1.20.9.1
Mo
contains
Chrysiogenes arsenatis
1.20.9.1
Zn
contains
Chrysiogenes arsenatis
1.20.99.1
Iron
contains several iron-sulfur centres as prosthetic groups
Chrysiogenes arsenatis
1.20.99.1
Zinc
it is possible that one zinc atom represents an additional cofactor
Chrysiogenes arsenatis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.20.9.1
29000
-
1 * 87000 + 1 * 29000, SDS-PAGE
Chrysiogenes arsenatis
1.20.9.1
87000
-
1 * 87000 + 1 * 29000, SDS-PAGE
Chrysiogenes arsenatis
1.20.9.1
123000
-
gel filtration
Chrysiogenes arsenatis
1.20.99.1
29000
-
alpha,beta, 1 * 87000, ArrA, + 1 * 29000, ArrB, SDS-PAGE
Chrysiogenes arsenatis
1.20.99.1
87000
-
alpha,beta, 1 * 87000, ArrA, + 1 * 29000, ArrB, SDS-PAGE
Chrysiogenes arsenatis
1.20.99.1
123000
-
gel filtration
Chrysiogenes arsenatis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.20.99.1
arsenate + acetate
Chrysiogenes arsenatis
anaerobic respiration using arsenate as terminal electron acceptor and acetate as the electron donor
arsenite + ?
-
Chrysiogenes arsenatis
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.20.9.1
-
Chrysiogenes arsenatis
1.20.99.1
-
Chrysiogenes arsenatis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.20.9.1
7013
-
-
Chrysiogenes arsenatis
1.20.99.1
116.1
-
-
Chrysiogenes arsenatis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.9.1
arsenate + azurin(red)
-
287859
Chrysiogenes arsenatis
arsenite + H2O + azurin(ox)
-
-
-
?
1.20.99.1
arsenate + acetate
specific for arsenate
287859
Chrysiogenes arsenatis
arsenite + ?
-
287859
Chrysiogenes arsenatis
?
1.20.99.1
arsenate + acetate
anaerobic respiration using arsenate as terminal electron acceptor and acetate as the electron donor
287859
Chrysiogenes arsenatis
arsenite + ?
-
287859
Chrysiogenes arsenatis
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.20.9.1
dimer
1 * 87000 + 1 * 29000, SDS-PAGE
Chrysiogenes arsenatis
1.20.99.1
dimer
alpha,beta, 1 * 87000, ArrA, + 1 * 29000, ArrB, SDS-PAGE
Chrysiogenes arsenatis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.20.9.1
14400
-
arsenate
-
Chrysiogenes arsenatis
1.20.99.1
14400
-
arsenate
-
Chrysiogenes arsenatis