BRENDA - Enzyme Database show

Crystal structure of the 100 kDa arsenite oxidase from Alcaligenes faecalis in two crystal forms at 1.64 A and 2.03 A

Ellis, P.J.; Conrads, T.; Hille, R.; Kuhn, P.; Structure 9, 125-132 (2001)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.20.99.1
additional information
induction by arsenite
Alcaligenes faecalis
Crystallization (Commentary)
EC Number
Crystallization
Organism
1.20.9.1
; crystal structure of arsenite oxidase to 2.03A in a P21 crystal form with two molecules in the asymmetric unit and to 1.64 A in a P1 crystal form with four molecules in the asymmetric unit. The large subunit contains a Mo site, consisting of a Mo atom bound to two pterin cofactors, and a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site; crystal structure of arsenite oxidase to 2.03A in a P21 crystal form with two molecules in the asymmetric unit and to 1.64 A in a P1 crystal form with four molecules in the asymmetric unit. The large subunit contains a Mo site, consisting of a Mo atom bound to two pterin cofactors, and a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site
Alcaligenes faecalis
1.20.99.1
X-ray diffraction structure determination and analysis of 2 different crystal forms at 1.64-2.03 A using multiple isomorphous replacement with anomalous scattering and multiple-wavelength anomalous dispersion methods
Alcaligenes faecalis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.20.9.1
Fe
contains a [3Fe-4S] cluster and a Rieske-type [2Fe-2S] site
Alcaligenes faecalis
1.20.9.1
Iron
the large subunit contains a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site; the large subunit contains a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site
Alcaligenes faecalis
1.20.9.1
Molybdenum
the large subunit contains a Mo site, consisting of a Mo atom bound to two pterin cofactors
Alcaligenes faecalis
1.20.99.1
Iron
enzyme is a MoFe protein, Mo atom bound to 2 pterin cofactors and a [3Fe4S] cluster, binding site on the large subunit, the small subunit contains a binding site for a Rieske-type [2Fe2S] cluster
Alcaligenes faecalis
1.20.99.1
Molybdenum
enzyme is a MoFe protein, Mo atom bound to 2 pterin cofactors and a [3Fe4S] cluster, binding site on the large subunit, the Mo atom is not covalently bound to the polypeptide
Alcaligenes faecalis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.20.99.1
arsenite + acceptor
Alcaligenes faecalis
detoxification of arsenite to the less toxic arsenate prior to methylation
arsenate + reduced acceptor
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.20.9.1
Alcaligenes faecalis
-
-
-
1.20.9.1
Alcaligenes faecalis
Q7SIF3
small subunit aoxA
-
1.20.9.1
Alcaligenes faecalis
Q7SIF4
large subunit aoxB
-
1.20.9.1
Alcaligenes faecalis NCIB 8687
Q7SIF3
small subunit aoxA
-
1.20.9.1
Alcaligenes faecalis NCIB 8687
Q7SIF4
large subunit aoxB
-
1.20.99.1
Alcaligenes faecalis
-
NCIB 8687
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.9.1
arsenite + H2O + azurin(oxidized)
-
287858
Alcaligenes faecalis
arsenate + azurin(reduced)
-
287858
Alcaligenes faecalis
ir
1.20.99.1
arsenite + acceptor
-
287858
Alcaligenes faecalis
arsenate + reduced acceptor
-
-
-
?
1.20.99.1
arsenite + acceptor
detoxification of arsenite to the less toxic arsenate prior to methylation
287858
Alcaligenes faecalis
arsenate + reduced acceptor
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.20.9.1
More
heterodimer
Alcaligenes faecalis
1.20.99.1
dimer
large subunit of 825 amino acid residues and a small subunit of 134 amino acid residues
Alcaligenes faecalis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.20.9.1
iron-sulfur centre
the large subunit contains a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site; the large subunit contains a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site
Alcaligenes faecalis
1.20.9.1
molybdopterin
the large subunit contains a Mo site, consisting of a Mo atom bound to two pterin cofactors
Alcaligenes faecalis
1.20.99.1
Pterin molybdenum cofactor
enzyme is a MoFe protein, Mo atom bound to 2 pterin cofactors and a [3Fe4S] cluster, binding site on the large subunit
Alcaligenes faecalis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.20.99.1
additional information
induction by arsenite
Alcaligenes faecalis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.20.9.1
iron-sulfur centre
the large subunit contains a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site
Alcaligenes faecalis
1.20.9.1
molybdopterin
the large subunit contains a Mo site, consisting of a Mo atom bound to two pterin cofactors
Alcaligenes faecalis
1.20.99.1
Pterin molybdenum cofactor
enzyme is a MoFe protein, Mo atom bound to 2 pterin cofactors and a [3Fe4S] cluster, binding site on the large subunit
Alcaligenes faecalis
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.20.9.1
-
Alcaligenes faecalis
1.20.9.1
crystal structure of arsenite oxidase to 2.03A in a P21 crystal form with two molecules in the asymmetric unit and to 1.64 A in a P1 crystal form with four molecules in the asymmetric unit. The large subunit contains a Mo site, consisting of a Mo atom bound to two pterin cofactors, and a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site
Alcaligenes faecalis
1.20.99.1
X-ray diffraction structure determination and analysis of 2 different crystal forms at 1.64-2.03 A using multiple isomorphous replacement with anomalous scattering and multiple-wavelength anomalous dispersion methods
Alcaligenes faecalis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.20.9.1
Fe
contains a [3Fe-4S] cluster and a Rieske-type [2Fe-2S] site
Alcaligenes faecalis
1.20.9.1
Iron
the large subunit contains a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site
Alcaligenes faecalis
1.20.9.1
Molybdenum
the large subunit contains a Mo site, consisting of a Mo atom bound to two pterin cofactors
Alcaligenes faecalis
1.20.99.1
Iron
enzyme is a MoFe protein, Mo atom bound to 2 pterin cofactors and a [3Fe4S] cluster, binding site on the large subunit, the small subunit contains a binding site for a Rieske-type [2Fe2S] cluster
Alcaligenes faecalis
1.20.99.1
Molybdenum
enzyme is a MoFe protein, Mo atom bound to 2 pterin cofactors and a [3Fe4S] cluster, binding site on the large subunit, the Mo atom is not covalently bound to the polypeptide
Alcaligenes faecalis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.20.99.1
arsenite + acceptor
Alcaligenes faecalis
detoxification of arsenite to the less toxic arsenate prior to methylation
arsenate + reduced acceptor
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.9.1
arsenite + H2O + azurin(oxidized)
-
287858
Alcaligenes faecalis
arsenate + azurin(reduced)
-
287858
Alcaligenes faecalis
ir
1.20.99.1
arsenite + acceptor
-
287858
Alcaligenes faecalis
arsenate + reduced acceptor
-
-
-
?
1.20.99.1
arsenite + acceptor
detoxification of arsenite to the less toxic arsenate prior to methylation
287858
Alcaligenes faecalis
arsenate + reduced acceptor
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.20.9.1
More
heterodimer
Alcaligenes faecalis
1.20.99.1
dimer
large subunit of 825 amino acid residues and a small subunit of 134 amino acid residues
Alcaligenes faecalis