BRENDA - Enzyme Database show

The purification and characterization of arsenite oxidase from Alcaligenes faecalis, a molybdenum-containing hydroxylase

Anderson, G.L.; Williams, J.; Hille, R.; J. Biol. Chem. 267, 23674-23682 (1992)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.9.1
0.008
-
azurin
-
Alcaligenes faecalis
1.20.9.1
0.07
-
arsenite
-
Alcaligenes faecalis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.20.9.1
Fe
contains 3 to 4 mol of Fe per 85 kD protein
Alcaligenes faecalis
1.20.9.1
Mo
contains 0.75 mol Mo per mol of protein; contains one Mo binding site
Alcaligenes faecalis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.20.9.1
85000
-
gel filtration
Alcaligenes faecalis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.20.9.1
Alcaligenes faecalis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.20.9.1
-
Alcaligenes faecalis
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.20.9.1
2.88
-
-
Alcaligenes faecalis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.9.1
arsenite + H2O + azurin(oxidized)
-
287857
Alcaligenes faecalis
arsenate + azurin(reduced)
-
287857
Alcaligenes faecalis
?
Subunits
EC Number
Subunits
Commentary
Organism
1.20.9.1
monomer
SDS-PAGE
Alcaligenes faecalis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.20.9.1
27
-
azurin
-
Alcaligenes faecalis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.20.9.1
6
-
-
Alcaligenes faecalis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.9.1
0.008
-
azurin
-
Alcaligenes faecalis
1.20.9.1
0.07
-
arsenite
-
Alcaligenes faecalis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.20.9.1
Fe
contains 3 to 4 mol of Fe per 85 kD protein
Alcaligenes faecalis
1.20.9.1
Mo
contains 0.75 mol Mo per mol of protein; contains one Mo binding site
Alcaligenes faecalis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.20.9.1
85000
-
gel filtration
Alcaligenes faecalis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.20.9.1
-
Alcaligenes faecalis
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.20.9.1
2.88
-
-
Alcaligenes faecalis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.9.1
arsenite + H2O + azurin(oxidized)
-
287857
Alcaligenes faecalis
arsenate + azurin(reduced)
-
287857
Alcaligenes faecalis
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.20.9.1
monomer
SDS-PAGE
Alcaligenes faecalis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.20.9.1
27
-
azurin
-
Alcaligenes faecalis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.20.9.1
6
-
-
Alcaligenes faecalis