Any feedback?
Literature summary extracted from
- Dehydrogenation of D-lactate by a soluble enzyme from kidney mitochondria (1959), Biochim. Biophys. Acta, 34, 290-291.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.6 | ferricyanide | - |
Oryctolagus cuniculus |  |
| 1.1.99.6 | methylene blue | - |
Oryctolagus cuniculus | |
| 1.1.99.6 | additional information | ageing of extracts increases dehydrogenase activity | Oryctolagus cuniculus | |
| 1.1.99.6 | O2 | no appreciable O2 uptake in absence of electron carrier | Oryctolagus cuniculus | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.6 | 1,10-phenanthroline | inhibition potentiated by KCN | Oryctolagus cuniculus | |
| 1.1.99.6 | Cu2+ | strong | Oryctolagus cuniculus | |
| 1.1.99.6 | EDTA | - |
Oryctolagus cuniculus | |
| 1.1.99.6 | Hg2+ | strong | Oryctolagus cuniculus | |
| 1.1.99.6 | KCN | potentiates inhibition of 1,10-phenanthroline | Oryctolagus cuniculus | |
| 1.1.99.6 | p-chloromercuribenzoate | strong | Oryctolagus cuniculus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.6 | 3.4 | - |
D-lactate | - |
Oryctolagus cuniculus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.99.6 | mitochondrion | - |
Oryctolagus cuniculus | 5739 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.6 | KCN | raises dehydrogenase activity of freshly prepared kidney extracts | Oryctolagus cuniculus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.6 | (R)-lactate + acceptor | Oryctolagus cuniculus | enzyme acts on a variety of (R)-2-hydroxy acids | pyruvate + reduced acceptor | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.6 | Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.99.6 | - |
Oryctolagus cuniculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.99.6 | kidney | - |
Oryctolagus cuniculus | - |
| 1.1.99.6 | liver | - |
Oryctolagus cuniculus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.6 | (R)-lactate + acceptor | acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide | Oryctolagus cuniculus | pyruvate + reduced acceptor | - |
? | |
| 1.1.99.6 | (R)-lactate + acceptor | enzyme acts on a variety of (R)-2-hydroxy acids | Oryctolagus cuniculus | pyruvate + reduced acceptor | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.6 | 20 | - |
assay at | Oryctolagus cuniculus |
| 1.1.99.6 | 30 | - |
assay at | Oryctolagus cuniculus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.6 | 8.1 | - |
- |
Oryctolagus cuniculus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.6 | 2,6-dichlorophenolindophenol | - |
Oryctolagus cuniculus | |
| 1.1.99.6 | cytochrome c | possible physiological acceptor | Oryctolagus cuniculus | |
| 1.1.99.6 | phenazine methosulfate | - |
Oryctolagus cuniculus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
