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Literature summary extracted from

  • Schryvers, A.; Weiner, J.H.
    The anaerobic sn-glycerol-3-phosphate dehydrogenase of Escherichia coli. Purification and characterization (1981), J. Biol. Chem., 256, 9959-9965.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.5.3 FAD stimulates the reaction with phenazine methosulfate and methylene blue. 20% increase in activity of the reaction with phenazine methosulfate with a half-maximal concentration of 200 nM Escherichia coli
1.1.5.3 FMN stimulates the reaction with phenazine methosulfate. 6fold increase in activity with a half-maximal concentration of 0.13 mM Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.5.3 1,10-phenanthroline
-
 Escherichia coli
1.1.5.3 DTNB
-
 Escherichia coli
1.1.5.3 FMN reaction with methylene blue Escherichia coli
1.1.5.3 PCMB
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.3 0.1
-
 glycerol 3-phosphate in absence of exogenous FMN Escherichia coli
1.1.5.3 0.339
-
 glycerol 3-phosphate in presence of exogenous FMN Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.3 membrane associated with membrane Escherichia coli 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.5.3 Iron contains 2 mol of non-heme iron per dimer Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.3 43000
-
 1 * 43000 + 1 * 62000, SDS-PAGE Escherichia coli
1.1.5.3 62000
-
 1 * 43000 + 1 * 62000, SDS-PAGE Escherichia coli
1.1.5.3 93500
-
 equilibrium sedimentation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.3 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.3
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.1.5.3 -70°C or 4°C, concentrated to 4-10 mg/ml, stable Escherichia coli
1.1.5.3 4°C, pH 6.0-8.5, 20% ethylene glycol v/v, 10% loss of activity after 60 h Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.3 sn-glycerol 3-phosphate + methylene blue
-
 Escherichia coli glycerone phosphate + reduced methylene blue
-
 ?
1.1.5.3 sn-glycerol 3-phosphate + phenazine methosulfate
-
 Escherichia coli glycerone phosphate + reduced phenazine methosulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.3 dimer 1 * 43000 + 1 * 62000, SDS-PAGE Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.5.3 33.3
-
 glycerol 3-phosphate
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.3 FAD contains 1 mol of noncovalently bound FAD per dimer Escherichia coli