BRENDA - Enzyme Database show

Metal-containing flavoprotein dehydrogenases

Hatefi, Y.; Stiggall, D.L.; The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ) 13, 175-297 (1976)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.3.17
NAD+
stimulation of choline oxidation
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.2.3
L-lactate
inhibition by excess of substrate, no inhibition with Saccharomyces cerevisiae enzyme
Wickerhamomyces anomalus
1.1.2.3
p-mercuribenzoate
-
Saccharomyces cerevisiae
1.1.2.4
o-phenanthroline
-
Saccharomyces cerevisiae
1.1.3.17
2-chloro-N-(2-chloroethyl)-N-methylethanamine
nitrogen mustard, strong competitive inhibitor
Rattus norvegicus
1.1.3.17
Amytal
-
Rattus norvegicus
1.1.3.17
Betaine aldehyde
-
Rattus norvegicus
1.1.3.17
rotenone
-
Rattus norvegicus
1.8.1.2
arsenite
-
Escherichia coli
1.8.1.2
arsenite
-
Saccharomyces cerevisiae
1.8.1.2
CN-
-
Neurospora crassa
1.8.1.2
CN-
-
Salmonella enterica subsp. enterica serovar Typhimurium
1.8.1.2
CN-
-
Saccharomyces cerevisiae
1.8.1.2
CN-
-
Escherichia coli
1.8.1.2
CO
-
Neurospora crassa
1.8.1.2
CO
-
Salmonella enterica subsp. enterica serovar Typhimurium
1.8.1.2
CO
-
Escherichia coli
1.8.1.2
CO
-
Saccharomyces cerevisiae
1.8.1.2
p-Mercuriphenylsulfonate
NADPH-dependent reactions
Escherichia coli
1.8.1.2
Sulfide
reduction of sulfite, nitrite, hydroxylamine by reduced methyl viologen
Escherichia coli
1.8.1.2
Sulfide
reduction of sulfite, nitrite, hydroxylamine by reduced methyl viologen
Salmonella enterica subsp. enterica serovar Typhimurium
1.8.1.2
Sulfide
reduction of sulfite, nitrite, hydroxylamine by reduced methyl viologen
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.2.4
0.0054
-
cytochrome c
-
Saccharomyces cerevisiae
1.1.2.4
0.285
-
D-lactate
-
Saccharomyces cerevisiae
1.1.2.4
1.4
-
D-2-hydroxybutyrate
-
Saccharomyces cerevisiae
1.1.2.4
4.45
-
phenazine methosulfate
-
Saccharomyces cerevisiae
1.1.3.17
7
-
choline
at 38°C and pH 7.6
Rattus norvegicus
1.7.1.4
0.000045
-
FAD
-
Torulopsis nitratophila
1.7.1.4
0.019
-
nitrite
-
Torulopsis nitratophila
1.8.1.2
0.0043
0.0074
sulfite
-
Escherichia coli
1.8.1.2
0.005
-
NADPH
-
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.2.4
mitochondrion
-
Saccharomyces cerevisiae
5739
-
1.1.3.17
mitochondrion
membrane-bound
Rattus norvegicus
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.2.3
Fe2+
bound to enzyme
Wickerhamomyces anomalus
1.1.2.3
Fe2+
bound to enzyme
Saccharomyces cerevisiae
1.1.2.4
Fe2+
restores activity after inhibition with o-phenanthroline
Saccharomyces cerevisiae
1.1.2.4
ferricyanide
not suitable as electron acceptor
Saccharomyces cerevisiae
1.1.2.4
Mn2+
restores activity after inhibition with o-phenanthroline
Saccharomyces cerevisiae
1.1.2.4
Zn2+
very tightly bound to enzyme
Saccharomyces cerevisiae
1.8.99.2
Iron
contains 6-8 gatom of nonheme iron
Desulfovibrio vulgaris
1.8.99.2
Iron
contains 6-11 gatom of nonheme iron
Thiobacillus denitrificans
1.8.99.2
Iron
contains 8-10 gatom of nonheme iron
Thiobacillus thioparus
1.8.99.2
Iron
contains 4 gatom of nonheme iron and 2 gatom of heme iron
Thiocapsa roseopersicina
1.8.99.2
Iron
-
Desulfovibrio desulfuricans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.2.3
58600
-
4 * 58600, calculated bases on amino acid sequence and heme extinction coefficient
Saccharomyces cerevisiae
1.1.2.3
61000
-
4 * 61000
Wickerhamomyces anomalus
1.1.2.3
235000
-
X-ray diffraction studies
Saccharomyces cerevisiae
1.1.2.4
100000
-
sedimentation equilibrium centrifugation
Saccharomyces cerevisiae
1.8.99.2
170000
-
-
Desulfovibrio desulfuricans
1.8.99.2
170000
-
-
Thiobacillus thioparus
1.8.99.2
180000
-
-
Thiobacillus denitrificans
1.8.99.2
180000
-
-
Thiocapsa roseopersicina
1.8.99.2
220000
-
-
Desulfovibrio vulgaris
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.2.3
(S)-lactate + 2 ferricytochrome c
Wickerhamomyces anomalus
-
pyruvate + 2 ferrocytochrome c + 2 H+
-
Wickerhamomyces anomalus
-
1.1.2.3
(S)-lactate + 2 ferricytochrome c
Saccharomyces cerevisiae
can feed electrons to respiratory chain at the level of cytochrome c
pyruvate + 2 ferrocytochrome c + 2 H+
-
Saccharomyces cerevisiae
-
1.1.2.4
(R)-lactate + 2 ferricytochrome c
Saccharomyces cerevisiae
-
pyruvate + ferrocytochrome c
-
Saccharomyces cerevisiae
-
1.1.3.17
choline + O2
Rattus norvegicus
-
betaine aldehyde + H2O2
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
Desulfovibrio vulgaris
-
AMP + sulfite + acceptor
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
Escherichia coli
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
AMP + sulfite + acceptor
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
Klebsiella aerogenes
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
AMP + sulfite + acceptor
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
Thiobacillus thioparus
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
AMP + sulfite + acceptor
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
Chromatium sp.
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
AMP + sulfite + acceptor
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
Desulfovibrio desulfuricans
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
AMP + sulfite + acceptor
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
Thiobacillus denitrificans
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
AMP + sulfite + acceptor
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
Acidithiobacillus thiooxidans
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
AMP + sulfite + acceptor
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
Desulfotomaculum nigrificans
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
AMP + sulfite + acceptor
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.2.3
Saccharomyces cerevisiae
-
-
-
1.1.2.3
Wickerhamomyces anomalus
-
-
-
1.1.2.4
Saccharomyces cerevisiae
-
-
-
1.1.3.17
Rattus norvegicus
-
rat
-
1.7.1.4
Torulopsis nitratophila
-
-
-
1.8.1.2
Escherichia coli
-
-
-
1.8.1.2
Neurospora crassa
-
-
-
1.8.1.2
Saccharomyces cerevisiae
-
-
-
1.8.1.2
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
1.8.99.2
Acidithiobacillus thiooxidans
-
-
-
1.8.99.2
Chromatium sp.
-
weak activity
-
1.8.99.2
Desulfotomaculum nigrificans
-
-
-
1.8.99.2
Desulfovibrio desulfuricans
-
-
-
1.8.99.2
Desulfovibrio vulgaris
-
-
-
1.8.99.2
Escherichia coli
-
-
-
1.8.99.2
Klebsiella aerogenes
-
weak activity
-
1.8.99.2
Thiobacillus denitrificans
-
-
-
1.8.99.2
Thiobacillus thioparus
-
-
-
1.8.99.2
Thiocapsa roseopersicina
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.3.17
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.2.3
(S)-lactate + 2 ferricytochrome c
-
287801
Wickerhamomyces anomalus
pyruvate + 2 ferrocytochrome c + 2 H+
-
287801
Wickerhamomyces anomalus
-
1.1.2.3
(S)-lactate + 2 ferricytochrome c
D-isomer not oxidized
287801
Saccharomyces cerevisiae
pyruvate + 2 ferrocytochrome c + 2 H+
-
287801
Saccharomyces cerevisiae
-
1.1.2.3
(S)-lactate + 2 ferricytochrome c
can feed electrons to respiratory chain at the level of cytochrome c
287801
Saccharomyces cerevisiae
pyruvate + 2 ferrocytochrome c + 2 H+
-
287801
Saccharomyces cerevisiae
-
1.1.2.3
additional information
2,6-dichloroindophenol, ferricyanide, methylene blue, 1,2-naphthoquinone and cytochrome c can serve as electron acceptors
287801
Saccharomyces cerevisiae
?
-
-
-
-
1.1.2.3
additional information
electron acceptors other than ferricytochrome c used
287801
Saccharomyces cerevisiae
?
-
-
-
-
1.1.2.3
additional information
electron acceptors other than ferricytochrome c used
287801
Wickerhamomyces anomalus
?
-
-
-
-
1.1.2.4
(R)-lactate + 2 ferricytochrome c
-
287801
Saccharomyces cerevisiae
pyruvate + ferrocytochrome c
-
287801
Saccharomyces cerevisiae
-
1.1.2.4
(R)-lactate + ferricytochrome c
-
287801
Saccharomyces cerevisiae
pyruvate + ferrocytochrome c
-
287801
Saccharomyces cerevisiae
-
1.1.2.4
D-2-hydroxybutyrate + cytochrome c
-
287801
Saccharomyces cerevisiae
2-oxobutanoate + ferrocytochrome c
-
-
-
?
1.1.3.17
choline + O2
-
287801
Rattus norvegicus
betaine aldehyde + H2O2
-
-
-
?
1.7.1.4
NAD(P)H + nitrite + H+
specific for NADPH, can utilize reduced benzyl or methyl viologen as electron donor
287801
Torulopsis nitratophila
NAD(P)+ + NH3 + H2O
-
287801
Torulopsis nitratophila
?
1.8.1.2
hydroxylamine + NADPH
-
287801
Escherichia coli
ammonia + NADP+
-
-
-
?
1.8.1.2
additional information
transfer of hydrogen from NADPH to 3-acetylpyridineadenine dinucleotide phosphate
287801
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
1.8.1.2
additional information
transfer of hydrogen from NADPH to 3-acetylpyridineadenine dinucleotide phosphate
287801
Escherichia coli
?
-
-
-
-
1.8.1.2
additional information
transfer of hydrogen from NADPH to 3-acetylpyridineadenine dinucleotide phosphate
287801
Saccharomyces cerevisiae
?
-
-
-
-
1.8.1.2
additional information
reduction of sulfite, nitrite, and hydroxylamine by NADPH requires catalytic activities of both subunits, reduction of sulfite, nitrite, and hydroxylamine by methyl viologen requires hemoprotein subunit
287801
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
1.8.1.2
additional information
reduction of sulfite, nitrite, and hydroxylamine by NADPH requires catalytic activities of both subunits, reduction of sulfite, nitrite, and hydroxylamine by methyl viologen requires hemoprotein subunit
287801
Escherichia coli
?
-
-
-
-
1.8.1.2
additional information
reduction of sulfite, nitrite, and hydroxylamine by NADPH requires catalytic activities of both subunits, reduction of sulfite, nitrite, and hydroxylamine by methyl viologen requires hemoprotein subunit
287801
Saccharomyces cerevisiae
?
-
-
-
-
1.8.1.2
additional information
reactions of FMN depleted enzyme
287801
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
1.8.1.2
additional information
reactions of FMN depleted enzyme
287801
Escherichia coli
?
-
-
-
-
1.8.1.2
additional information
reactions of FMN depleted enzyme
287801
Saccharomyces cerevisiae
?
-
-
-
-
1.8.1.2
additional information
reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2
287801
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
1.8.1.2
additional information
reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2
287801
Escherichia coli
?
-
-
-
-
1.8.1.2
additional information
reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2
287801
Saccharomyces cerevisiae
?
-
-
-
-
1.8.1.2
nitrite + NADPH
-
287801
Salmonella enterica subsp. enterica serovar Typhimurium
ammonia + NADP+
-
-
-
?
1.8.1.2
nitrite + NADPH
-
287801
Escherichia coli
ammonia + NADP+
-
-
-
?
1.8.1.2
nitrite + NADPH
-
287801
Saccharomyces cerevisiae
ammonia + NADP+
-
-
-
?
1.8.1.2
nitrite + NADPH
-
287801
Neurospora crassa
ammonia + NADP+
-
-
-
?
1.8.1.2
sulfite + NADPH
reduction of sulfite by reduced methyl viologen is catalyzed by hemoprotein subunit alone
287801
Salmonella enterica subsp. enterica serovar Typhimurium
sulfide + NADP+ + H2O
-
-
-
-
1.8.1.2
sulfite + NADPH
reduction of sulfite by reduced methyl viologen is catalyzed by hemoprotein subunit alone
287801
Escherichia coli
sulfide + NADP+ + H2O
-
-
-
-
1.8.1.2
sulfite + NADPH
reduction of sulfite by reduced methyl viologen is catalyzed by hemoprotein subunit alone
287801
Saccharomyces cerevisiae
sulfide + NADP+ + H2O
-
-
-
-
1.8.99.2
adenylyl sulfate + reduced acceptor
-
287801
Desulfovibrio vulgaris
AMP + sulfite + acceptor
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
287801
Escherichia coli
AMP + sulfite + acceptor
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
287801
Klebsiella aerogenes
AMP + sulfite + acceptor
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
287801
Thiobacillus thioparus
AMP + sulfite + acceptor
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
287801
Chromatium sp.
AMP + sulfite + acceptor
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
287801
Desulfovibrio desulfuricans
AMP + sulfite + acceptor
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
287801
Thiobacillus denitrificans
AMP + sulfite + acceptor
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
287801
Acidithiobacillus thiooxidans
AMP + sulfite + acceptor
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
287801
Desulfotomaculum nigrificans
AMP + sulfite + acceptor
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced methyl viologen
-
287801
Escherichia coli
AMP + sulfite + methyl viologen
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced methyl viologen
-
287801
Klebsiella aerogenes
AMP + sulfite + methyl viologen
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced methyl viologen
-
287801
Thiobacillus thioparus
AMP + sulfite + methyl viologen
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced methyl viologen
-
287801
Chromatium sp.
AMP + sulfite + methyl viologen
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced methyl viologen
-
287801
Desulfovibrio desulfuricans
AMP + sulfite + methyl viologen
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced methyl viologen
-
287801
Thiobacillus denitrificans
AMP + sulfite + methyl viologen
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced methyl viologen
-
287801
Desulfovibrio vulgaris
AMP + sulfite + methyl viologen
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced methyl viologen
-
287801
Acidithiobacillus thiooxidans
AMP + sulfite + methyl viologen
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced methyl viologen
-
287801
Desulfotomaculum nigrificans
AMP + sulfite + methyl viologen
-
-
-
?
1.8.99.2
AMP + sulfite + cytochrome c
-
287801
Thiobacillus thioparus
adenylyl sulfate + ?
-
-
-
?
1.8.99.2
AMP + sulfite + cytochrome c
-
287801
Desulfovibrio vulgaris
adenylyl sulfate + ?
-
-
-
?
1.8.99.2
AMP + sulfite + cytochrome c
-
287801
Thiocapsa roseopersicina
adenylyl sulfate + ?
-
-
-
?
1.8.99.2
AMP + sulfite + ferricyanide
-
287801
Thiobacillus thioparus
adenylyl sulfate + ferrocyanide
-
-
-
?
1.8.99.2
AMP + sulfite + ferricyanide
-
287801
Desulfovibrio desulfuricans
adenylyl sulfate + ferrocyanide
-
-
-
?
1.8.99.2
AMP + sulfite + ferricyanide
-
287801
Thiobacillus denitrificans
adenylyl sulfate + ferrocyanide
-
-
-
?
1.8.99.2
AMP + sulfite + ferricyanide
-
287801
Desulfovibrio vulgaris
adenylyl sulfate + ferrocyanide
-
-
-
?
1.8.99.2
AMP + sulfite + ferricyanide
-
287801
Thiocapsa roseopersicina
adenylyl sulfate + ferrocyanide
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.2.3
tetramer
4 * 58600, calculated bases on amino acid sequence and heme extinction coefficient
Saccharomyces cerevisiae
1.1.2.3
tetramer
4 * 61000
Wickerhamomyces anomalus
1.8.1.2
dodecamer
alpha8,beta4, 8 * 58000-60000 + 4 * 54000-57000, sedimentation under denaturing conditions
Escherichia coli
1.8.1.2
More
dissociation of enzyme by 5 m urea into flavoprotein octamer and hemoprotein monomers
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.99.2
7.2
-
reaction with AMP, SO32- and ferricyanide
Thiobacillus denitrificans
1.8.99.2
7.4
-
-
Desulfovibrio desulfuricans
1.8.99.2
7.4
-
reaction with AMP, SO32- and ferricyanide
Desulfovibrio vulgaris
1.8.99.2
7.4
-
-
Thiobacillus thioparus
1.8.99.2
8
-
reaction with AMP, SO32- and ferricyanide
Thiocapsa roseopersicina
1.8.99.2
9
-
-
Desulfovibrio vulgaris
1.8.99.2
9
-
reaction with AMP, SO32- and cytochrome c
Thiocapsa roseopersicina
1.8.99.2
9.5
-
-
Desulfovibrio vulgaris
1.8.99.2
9.5
-
reaction with AMP, SO32- and cytochrome c
Thiobacillus thioparus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.2.3
cytochrome c
-
Wickerhamomyces anomalus
1.1.2.3
cytochrome c
-
Saccharomyces cerevisiae
1.1.2.3
FMN
one FMN per subunit
Wickerhamomyces anomalus
1.1.2.3
FMN
one FMN per subunit
Saccharomyces cerevisiae
1.1.2.3
heme
one heme per subunit
Wickerhamomyces anomalus
1.1.2.3
heme
one heme per subunit
Saccharomyces cerevisiae
1.1.2.3
additional information
2,6-dichloroindophenol, ferricyanide, methylene blue, 1,2-naphthoquinone and cytochrome c can serve as electron acceptors; electron acceptors other than ferricytochrome c used
Saccharomyces cerevisiae
1.1.2.3
additional information
electron acceptors other than ferricytochrome c used
Wickerhamomyces anomalus
1.1.2.4
2,6-dichlorophenolindophenol
can substitute for cytochrome c
Saccharomyces cerevisiae
1.1.2.4
cytochrome c
-
Saccharomyces cerevisiae
1.1.2.4
FAD
-
Saccharomyces cerevisiae
1.1.2.4
phenazine methosulfate
can substitute for cytochrome c
Saccharomyces cerevisiae
1.8.99.2
FAD
enzyme contains 1 mol of FAD per mol of enzyme
Thiobacillus denitrificans
1.8.99.2
FAD
enzyme contains 1 mol of FAD per mol of enzyme
Thiobacillus thioparus
1.8.99.2
FAD
enzyme contains 1 mol of FAD per mol of enzyme
Thiocapsa roseopersicina
1.8.99.2
FAD
enzyme contains 1 mol of FAD per mol of enzyme
Desulfovibrio desulfuricans
1.8.99.2
FAD
enzyme contains 1 mol of FAD per mol of enzyme
Desulfovibrio vulgaris
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.3.17
2
-
Betaine aldehyde
oxidation of choline is competitively inhibited
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.3.17
NAD+
stimulation of choline oxidation
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.2.3
cytochrome c
-
Wickerhamomyces anomalus
1.1.2.3
cytochrome c
-
Saccharomyces cerevisiae
1.1.2.3
FMN
one FMN per subunit
Wickerhamomyces anomalus
1.1.2.3
FMN
one FMN per subunit
Saccharomyces cerevisiae
1.1.2.3
heme
one heme per subunit
Wickerhamomyces anomalus
1.1.2.3
heme
one heme per subunit
Saccharomyces cerevisiae
1.1.2.3
additional information
2,6-dichloroindophenol, ferricyanide, methylene blue, 1,2-naphthoquinone and cytochrome c can serve as electron acceptors; electron acceptors other than ferricytochrome c used
Saccharomyces cerevisiae
1.1.2.3
additional information
electron acceptors other than ferricytochrome c used
Wickerhamomyces anomalus
1.1.2.4
2,6-dichlorophenolindophenol
can substitute for cytochrome c
Saccharomyces cerevisiae
1.1.2.4
cytochrome c
-
Saccharomyces cerevisiae
1.1.2.4
FAD
-
Saccharomyces cerevisiae
1.1.2.4
phenazine methosulfate
can substitute for cytochrome c
Saccharomyces cerevisiae
1.8.99.2
FAD
enzyme contains 1 mol of FAD per mol of enzyme
Thiobacillus denitrificans
1.8.99.2
FAD
enzyme contains 1 mol of FAD per mol of enzyme
Thiobacillus thioparus
1.8.99.2
FAD
enzyme contains 1 mol of FAD per mol of enzyme
Thiocapsa roseopersicina
1.8.99.2
FAD
enzyme contains 1 mol of FAD per mol of enzyme
Desulfovibrio desulfuricans
1.8.99.2
FAD
enzyme contains 1 mol of FAD per mol of enzyme
Desulfovibrio vulgaris
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.2.3
L-lactate
inhibition by excess of substrate, no inhibition with Saccharomyces cerevisiae enzyme
Wickerhamomyces anomalus
1.1.2.3
p-mercuribenzoate
-
Saccharomyces cerevisiae
1.1.2.4
o-phenanthroline
-
Saccharomyces cerevisiae
1.1.3.17
2-chloro-N-(2-chloroethyl)-N-methylethanamine
nitrogen mustard, strong competitive inhibitor
Rattus norvegicus
1.1.3.17
Amytal
-
Rattus norvegicus
1.1.3.17
Betaine aldehyde
-
Rattus norvegicus
1.1.3.17
rotenone
-
Rattus norvegicus
1.8.1.2
arsenite
-
Escherichia coli
1.8.1.2
arsenite
-
Saccharomyces cerevisiae
1.8.1.2
CN-
-
Neurospora crassa
1.8.1.2
CN-
-
Salmonella enterica subsp. enterica serovar Typhimurium
1.8.1.2
CN-
-
Saccharomyces cerevisiae
1.8.1.2
CN-
-
Escherichia coli
1.8.1.2
CO
-
Neurospora crassa
1.8.1.2
CO
-
Salmonella enterica subsp. enterica serovar Typhimurium
1.8.1.2
CO
-
Escherichia coli
1.8.1.2
CO
-
Saccharomyces cerevisiae
1.8.1.2
p-Mercuriphenylsulfonate
NADPH-dependent reactions
Escherichia coli
1.8.1.2
Sulfide
reduction of sulfite, nitrite, hydroxylamine by reduced methyl viologen
Escherichia coli
1.8.1.2
Sulfide
reduction of sulfite, nitrite, hydroxylamine by reduced methyl viologen
Salmonella enterica subsp. enterica serovar Typhimurium
1.8.1.2
Sulfide
reduction of sulfite, nitrite, hydroxylamine by reduced methyl viologen
Saccharomyces cerevisiae
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.1.3.17
2
-
Betaine aldehyde
oxidation of choline is competitively inhibited
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.2.4
0.0054
-
cytochrome c
-
Saccharomyces cerevisiae
1.1.2.4
0.285
-
D-lactate
-
Saccharomyces cerevisiae
1.1.2.4
1.4
-
D-2-hydroxybutyrate
-
Saccharomyces cerevisiae
1.1.2.4
4.45
-
phenazine methosulfate
-
Saccharomyces cerevisiae
1.1.3.17
7
-
choline
at 38°C and pH 7.6
Rattus norvegicus
1.7.1.4
0.000045
-
FAD
-
Torulopsis nitratophila
1.7.1.4
0.019
-
nitrite
-
Torulopsis nitratophila
1.8.1.2
0.0043
0.0074
sulfite
-
Escherichia coli
1.8.1.2
0.005
-
NADPH
-
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.2.4
mitochondrion
-
Saccharomyces cerevisiae
5739
-
1.1.3.17
mitochondrion
membrane-bound
Rattus norvegicus
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.2.3
Fe2+
bound to enzyme
Wickerhamomyces anomalus
1.1.2.3
Fe2+
bound to enzyme
Saccharomyces cerevisiae
1.1.2.4
Fe2+
restores activity after inhibition with o-phenanthroline
Saccharomyces cerevisiae
1.1.2.4
ferricyanide
not suitable as electron acceptor
Saccharomyces cerevisiae
1.1.2.4
Mn2+
restores activity after inhibition with o-phenanthroline
Saccharomyces cerevisiae
1.1.2.4
Zn2+
very tightly bound to enzyme
Saccharomyces cerevisiae
1.8.99.2
Iron
contains 6-8 gatom of nonheme iron
Desulfovibrio vulgaris
1.8.99.2
Iron
contains 6-11 gatom of nonheme iron
Thiobacillus denitrificans
1.8.99.2
Iron
contains 8-10 gatom of nonheme iron
Thiobacillus thioparus
1.8.99.2
Iron
contains 4 gatom of nonheme iron and 2 gatom of heme iron
Thiocapsa roseopersicina
1.8.99.2
Iron
-
Desulfovibrio desulfuricans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.2.3
58600
-
4 * 58600, calculated bases on amino acid sequence and heme extinction coefficient
Saccharomyces cerevisiae
1.1.2.3
61000
-
4 * 61000
Wickerhamomyces anomalus
1.1.2.3
235000
-
X-ray diffraction studies
Saccharomyces cerevisiae
1.1.2.4
100000
-
sedimentation equilibrium centrifugation
Saccharomyces cerevisiae
1.8.99.2
170000
-
-
Desulfovibrio desulfuricans
1.8.99.2
170000
-
-
Thiobacillus thioparus
1.8.99.2
180000
-
-
Thiobacillus denitrificans
1.8.99.2
180000
-
-
Thiocapsa roseopersicina
1.8.99.2
220000
-
-
Desulfovibrio vulgaris
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.2.3
(S)-lactate + 2 ferricytochrome c
Wickerhamomyces anomalus
-
pyruvate + 2 ferrocytochrome c + 2 H+
-
Wickerhamomyces anomalus
-
1.1.2.3
(S)-lactate + 2 ferricytochrome c
Saccharomyces cerevisiae
can feed electrons to respiratory chain at the level of cytochrome c
pyruvate + 2 ferrocytochrome c + 2 H+
-
Saccharomyces cerevisiae
-
1.1.2.4
(R)-lactate + 2 ferricytochrome c
Saccharomyces cerevisiae
-
pyruvate + ferrocytochrome c
-
Saccharomyces cerevisiae
-
1.1.3.17
choline + O2
Rattus norvegicus
-
betaine aldehyde + H2O2
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
Desulfovibrio vulgaris
-
AMP + sulfite + acceptor
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
Escherichia coli
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
AMP + sulfite + acceptor
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
Klebsiella aerogenes
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
AMP + sulfite + acceptor
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
Thiobacillus thioparus
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
AMP + sulfite + acceptor
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
Chromatium sp.
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
AMP + sulfite + acceptor
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
Desulfovibrio desulfuricans
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
AMP + sulfite + acceptor
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
Thiobacillus denitrificans
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
AMP + sulfite + acceptor
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
Acidithiobacillus thiooxidans
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
AMP + sulfite + acceptor
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
Desulfotomaculum nigrificans
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
AMP + sulfite + acceptor
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.3.17
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.2.3
(S)-lactate + 2 ferricytochrome c
-
287801
Wickerhamomyces anomalus
pyruvate + 2 ferrocytochrome c + 2 H+
-
287801
Wickerhamomyces anomalus
-
1.1.2.3
(S)-lactate + 2 ferricytochrome c
D-isomer not oxidized
287801
Saccharomyces cerevisiae
pyruvate + 2 ferrocytochrome c + 2 H+
-
287801
Saccharomyces cerevisiae
-
1.1.2.3
(S)-lactate + 2 ferricytochrome c
can feed electrons to respiratory chain at the level of cytochrome c
287801
Saccharomyces cerevisiae
pyruvate + 2 ferrocytochrome c + 2 H+
-
287801
Saccharomyces cerevisiae
-
1.1.2.3
additional information
2,6-dichloroindophenol, ferricyanide, methylene blue, 1,2-naphthoquinone and cytochrome c can serve as electron acceptors
287801
Saccharomyces cerevisiae
?
-
-
-
-
1.1.2.3
additional information
electron acceptors other than ferricytochrome c used
287801
Saccharomyces cerevisiae
?
-
-
-
-
1.1.2.3
additional information
electron acceptors other than ferricytochrome c used
287801
Wickerhamomyces anomalus
?
-
-
-
-
1.1.2.4
(R)-lactate + 2 ferricytochrome c
-
287801
Saccharomyces cerevisiae
pyruvate + ferrocytochrome c
-
287801
Saccharomyces cerevisiae
-
1.1.2.4
(R)-lactate + ferricytochrome c
-
287801
Saccharomyces cerevisiae
pyruvate + ferrocytochrome c
-
287801
Saccharomyces cerevisiae
-
1.1.2.4
D-2-hydroxybutyrate + cytochrome c
-
287801
Saccharomyces cerevisiae
2-oxobutanoate + ferrocytochrome c
-
-
-
?
1.1.3.17
choline + O2
-
287801
Rattus norvegicus
betaine aldehyde + H2O2
-
-
-
?
1.7.1.4
NAD(P)H + nitrite + H+
specific for NADPH, can utilize reduced benzyl or methyl viologen as electron donor
287801
Torulopsis nitratophila
NAD(P)+ + NH3 + H2O
-
287801
Torulopsis nitratophila
?
1.8.1.2
hydroxylamine + NADPH
-
287801
Escherichia coli
ammonia + NADP+
-
-
-
?
1.8.1.2
additional information
transfer of hydrogen from NADPH to 3-acetylpyridineadenine dinucleotide phosphate
287801
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
1.8.1.2
additional information
transfer of hydrogen from NADPH to 3-acetylpyridineadenine dinucleotide phosphate
287801
Escherichia coli
?
-
-
-
-
1.8.1.2
additional information
transfer of hydrogen from NADPH to 3-acetylpyridineadenine dinucleotide phosphate
287801
Saccharomyces cerevisiae
?
-
-
-
-
1.8.1.2
additional information
reduction of sulfite, nitrite, and hydroxylamine by NADPH requires catalytic activities of both subunits, reduction of sulfite, nitrite, and hydroxylamine by methyl viologen requires hemoprotein subunit
287801
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
1.8.1.2
additional information
reduction of sulfite, nitrite, and hydroxylamine by NADPH requires catalytic activities of both subunits, reduction of sulfite, nitrite, and hydroxylamine by methyl viologen requires hemoprotein subunit
287801
Escherichia coli
?
-
-
-
-
1.8.1.2
additional information
reduction of sulfite, nitrite, and hydroxylamine by NADPH requires catalytic activities of both subunits, reduction of sulfite, nitrite, and hydroxylamine by methyl viologen requires hemoprotein subunit
287801
Saccharomyces cerevisiae
?
-
-
-
-
1.8.1.2
additional information
reactions of FMN depleted enzyme
287801
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
1.8.1.2
additional information
reactions of FMN depleted enzyme
287801
Escherichia coli
?
-
-
-
-
1.8.1.2
additional information
reactions of FMN depleted enzyme
287801
Saccharomyces cerevisiae
?
-
-
-
-
1.8.1.2
additional information
reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2
287801
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
1.8.1.2
additional information
reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2
287801
Escherichia coli
?
-
-
-
-
1.8.1.2
additional information
reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2
287801
Saccharomyces cerevisiae
?
-
-
-
-
1.8.1.2
nitrite + NADPH
-
287801
Salmonella enterica subsp. enterica serovar Typhimurium
ammonia + NADP+
-
-
-
?
1.8.1.2
nitrite + NADPH
-
287801
Escherichia coli
ammonia + NADP+
-
-
-
?
1.8.1.2
nitrite + NADPH
-
287801
Saccharomyces cerevisiae
ammonia + NADP+
-
-
-
?
1.8.1.2
nitrite + NADPH
-
287801
Neurospora crassa
ammonia + NADP+
-
-
-
?
1.8.1.2
sulfite + NADPH
reduction of sulfite by reduced methyl viologen is catalyzed by hemoprotein subunit alone
287801
Salmonella enterica subsp. enterica serovar Typhimurium
sulfide + NADP+ + H2O
-
-
-
-
1.8.1.2
sulfite + NADPH
reduction of sulfite by reduced methyl viologen is catalyzed by hemoprotein subunit alone
287801
Escherichia coli
sulfide + NADP+ + H2O
-
-
-
-
1.8.1.2
sulfite + NADPH
reduction of sulfite by reduced methyl viologen is catalyzed by hemoprotein subunit alone
287801
Saccharomyces cerevisiae
sulfide + NADP+ + H2O
-
-
-
-
1.8.99.2
adenylyl sulfate + reduced acceptor
-
287801
Desulfovibrio vulgaris
AMP + sulfite + acceptor
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
287801
Escherichia coli
AMP + sulfite + acceptor
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
287801
Klebsiella aerogenes
AMP + sulfite + acceptor
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
287801
Thiobacillus thioparus
AMP + sulfite + acceptor
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
287801
Chromatium sp.
AMP + sulfite + acceptor
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
287801
Desulfovibrio desulfuricans
AMP + sulfite + acceptor
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
287801
Thiobacillus denitrificans
AMP + sulfite + acceptor
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
287801
Acidithiobacillus thiooxidans
AMP + sulfite + acceptor
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced acceptor
enzyme reduces adenylyl sulfate that is formed during dissimilatory reduction of sulfate
287801
Desulfotomaculum nigrificans
AMP + sulfite + acceptor
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced methyl viologen
-
287801
Escherichia coli
AMP + sulfite + methyl viologen
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced methyl viologen
-
287801
Klebsiella aerogenes
AMP + sulfite + methyl viologen
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced methyl viologen
-
287801
Thiobacillus thioparus
AMP + sulfite + methyl viologen
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced methyl viologen
-
287801
Chromatium sp.
AMP + sulfite + methyl viologen
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced methyl viologen
-
287801
Desulfovibrio desulfuricans
AMP + sulfite + methyl viologen
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced methyl viologen
-
287801
Thiobacillus denitrificans
AMP + sulfite + methyl viologen
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced methyl viologen
-
287801
Desulfovibrio vulgaris
AMP + sulfite + methyl viologen
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced methyl viologen
-
287801
Acidithiobacillus thiooxidans
AMP + sulfite + methyl viologen
-
-
-
?
1.8.99.2
adenylyl sulfate + reduced methyl viologen
-
287801
Desulfotomaculum nigrificans
AMP + sulfite + methyl viologen
-
-
-
?
1.8.99.2
AMP + sulfite + cytochrome c
-
287801
Thiobacillus thioparus
adenylyl sulfate + ?
-
-
-
?
1.8.99.2
AMP + sulfite + cytochrome c
-
287801
Desulfovibrio vulgaris
adenylyl sulfate + ?
-
-
-
?
1.8.99.2
AMP + sulfite + cytochrome c
-
287801
Thiocapsa roseopersicina
adenylyl sulfate + ?
-
-
-
?
1.8.99.2
AMP + sulfite + ferricyanide
-
287801
Thiobacillus thioparus
adenylyl sulfate + ferrocyanide
-
-
-
?
1.8.99.2
AMP + sulfite + ferricyanide
-
287801
Desulfovibrio desulfuricans
adenylyl sulfate + ferrocyanide
-
-
-
?
1.8.99.2
AMP + sulfite + ferricyanide
-
287801
Thiobacillus denitrificans
adenylyl sulfate + ferrocyanide
-
-
-
?
1.8.99.2
AMP + sulfite + ferricyanide
-
287801
Desulfovibrio vulgaris
adenylyl sulfate + ferrocyanide
-
-
-
?
1.8.99.2
AMP + sulfite + ferricyanide
-
287801
Thiocapsa roseopersicina
adenylyl sulfate + ferrocyanide
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.2.3
tetramer
4 * 58600, calculated bases on amino acid sequence and heme extinction coefficient
Saccharomyces cerevisiae
1.1.2.3
tetramer
4 * 61000
Wickerhamomyces anomalus
1.8.1.2
dodecamer
alpha8,beta4, 8 * 58000-60000 + 4 * 54000-57000, sedimentation under denaturing conditions
Escherichia coli
1.8.1.2
More
dissociation of enzyme by 5 m urea into flavoprotein octamer and hemoprotein monomers
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.8.99.2
7.2
-
reaction with AMP, SO32- and ferricyanide
Thiobacillus denitrificans
1.8.99.2
7.4
-
-
Desulfovibrio desulfuricans
1.8.99.2
7.4
-
reaction with AMP, SO32- and ferricyanide
Desulfovibrio vulgaris
1.8.99.2
7.4
-
-
Thiobacillus thioparus
1.8.99.2
8
-
reaction with AMP, SO32- and ferricyanide
Thiocapsa roseopersicina
1.8.99.2
9
-
-
Desulfovibrio vulgaris
1.8.99.2
9
-
reaction with AMP, SO32- and cytochrome c
Thiocapsa roseopersicina
1.8.99.2
9.5
-
-
Desulfovibrio vulgaris
1.8.99.2
9.5
-
reaction with AMP, SO32- and cytochrome c
Thiobacillus thioparus