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Literature summary extracted from

  • Shimao, M.; Tamogami, T.; Kishida, S.; Harayama, S.
    The gene pvaB encodes oxidized polyvinyl alcohol hydrolase of Pseudomonas sp. strain VM15C and forms an operon with the polyvinyl alcohol dehydrogenase gene pvaA (2000), Microbiology, 146, 649-657.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.7.1.7 expressed in Escherichia coli Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.7.1.7 PMSF sugggesting that the enzyme is a serine hydrolase Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.7.1.7 membrane specific activity in membrane fraction is several times higher than that of soluble fraction Pseudomonas sp. 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.7 40610
-
 deduced from amino acid sequence Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.6 Pseudomonas sp. P77931
-
-
1.1.2.6 Pseudomonas sp. VM15C P77931
-
-
3.7.1.7 Pseudomonas sp.
-
 VM15C
-
3.7.1.7 Pseudomonas sp. VM15C
-
 VM15C
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.7.1.7 nonane-4,6-dione + H2O = pentan-2-one + butanoate putative serine hydrolase Pseudomonas sp.
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7.1.7 0.33
-
 4,6-nonandione as substrate Pseudomonas sp.
3.7.1.7 1.65
-
 oxidized polyvinyl alcohol as substrate Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.6 polyvinyl alcohol + acceptor the enzyme introduces beta-dioxo groups into the polyvinyl alcohol molecule. The saturation level of beta-diketone groups introduced into polyvinyl alcohol is about 3.5% of the total vinyl alcohol units Pseudomonas sp. polyvinyl alcohol containing beta-dioxo groups + reduced acceptor
-
 ?
1.1.2.6 polyvinyl alcohol + acceptor the enzyme introduces beta-dioxo groups into the polyvinyl alcohol molecule. The saturation level of beta-diketone groups introduced into polyvinyl alcohol is about 3.5% of the total vinyl alcohol units Pseudomonas sp. VM15C polyvinyl alcohol containing beta-dioxo groups + reduced acceptor
-
 ?
3.7.1.7 4,6-nonanedione + H2O
-
 Pseudomonas sp. butyric acid + 2-pentanone
-
 ?
3.7.1.7 4,6-nonanedione + H2O
-
 Pseudomonas sp. VM15C butyric acid + 2-pentanone
-
 ?
3.7.1.7 polyvinyl alcohol + H2O
-
 Pseudomonas sp. carboxylic acid + methyl ketone
-
 ?
3.7.1.7 polyvinyl alcohol + H2O
-
 Pseudomonas sp. VM15C carboxylic acid + methyl ketone
-
 ?

Subunits

EC Number Subunits Comment Organism
3.7.1.7 monomer expressed in Escherichia coli Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.2.6 pvaA
-
 Pseudomonas sp.
1.1.2.6 PVADH
-
 Pseudomonas sp.

General Information

EC Number General Information Comment Organism
1.1.2.6 metabolism the gene pvaB encodes oxidized polyvinyl alcohol hydrolase and forms an operon with the polyvinyl alcohol dehydrogenase gene pvaA Pseudomonas sp.