EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.18 | Ag+ | 2 mM, 70% inhibition after 6 h | Schizophyllum commune | |
1.1.99.18 | Hg2+ | 2 mM, 20% inhibition after 6 h | Schizophyllum commune | |
1.1.99.18 | SDS | 5 mM, complete inhibition | Schizophyllum commune | |
1.1.99.18 | Zn2+ | 2 mM, 60% inhibition after 6 h | Schizophyllum commune |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.18 | 0.0028 | - |
cytochrome c | - |
Schizophyllum commune | |
1.1.99.18 | 0.01 | - |
2,6-dichlorphenolindophenol | - |
Schizophyllum commune | |
1.1.99.18 | 0.03 | - |
cellobiose | - |
Schizophyllum commune | |
1.1.99.18 | 0.058 | - |
cellotriose | - |
Schizophyllum commune | |
1.1.99.18 | 0.06 | - |
cellotetraose | - |
Schizophyllum commune | |
1.1.99.18 | 0.071 | - |
ferricyanide | - |
Schizophyllum commune | |
1.1.99.18 | 0.085 | - |
cellopentaose | - |
Schizophyllum commune | |
1.1.99.18 | 0.108 | - |
lactose | - |
Schizophyllum commune |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.99.18 | extracellular | - |
Schizophyllum commune | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 102000 | - |
x * 102000, SDS-PAGE | Schizophyllum commune |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.99.18 | Schizophyllum commune | - |
white-rot fungus | - |
1.1.99.18 | Schizophyllum commune AS 5.391 | - |
white-rot fungus | - |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.1.99.18 | very stable against H2O2 inactivation, 25 mM H2O2, pH 5.0, no loss in activity after 24 h, at 50 mM H2O2 27% enzyme activity are lost within 12 h, at 1 mM H2O2 with addition of 2 mM cellobiose and 0.05 mM FeCl3 all enzyme activity is lost within 4 h | Schizophyllum commune |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.1.99.18 | glycoprotein | 14% carbohydrate content | Schizophyllum commune |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.99.18 | ammonium sulfate, DEAE-Sephadex A50, Sephadex G-100 | Schizophyllum commune |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.99.18 | culture medium | - |
Schizophyllum commune | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 11.4 | - |
- |
Schizophyllum commune |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.99.18 | 4°C, several months | Schizophyllum commune |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.99.18 | cellobiose + 2,6-dichlorophenolindophenol | - |
Schizophyllum commune | cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
r | |
1.1.99.18 | cellobiose + acceptor | electron acceptors: cytochrome c, 2,6-dichlorphenolindophenol, ferricyanide. O2 is reduced to H2O2 | Schizophyllum commune | cellobiono-1,5-lactone + reduced acceptor | - |
? | |
1.1.99.18 | cellobiose + acceptor | electron acceptors: cytochrome c, 2,6-dichlorphenolindophenol, ferricyanide. O2 is reduced to H2O2 | Schizophyllum commune AS 5.391 | cellobiono-1,5-lactone + reduced acceptor | - |
? | |
1.1.99.18 | cellobiose + cytochrome c | - |
Schizophyllum commune | cellobiono-1,5-lactone + reduced cytochrome c | - |
r | |
1.1.99.18 | cellobiose + ferricyanide | - |
Schizophyllum commune | cellobiono-1,5-lactone + ferrocyanide | - |
r | |
1.1.99.18 | cellopentaose + acceptor | - |
Schizophyllum commune | cellopentaono-1,5-lactone + reduced acceptor | - |
? | |
1.1.99.18 | cellopentaose + acceptor | - |
Schizophyllum commune AS 5.391 | cellopentaono-1,5-lactone + reduced acceptor | - |
? | |
1.1.99.18 | cellotetraose + acceptor | - |
Schizophyllum commune | cellotetrono-1,5-lactone + reduced acceptor | - |
? | |
1.1.99.18 | cellotetraose + acceptor | - |
Schizophyllum commune AS 5.391 | cellotetrono-1,5-lactone + reduced acceptor | - |
? | |
1.1.99.18 | cellotriose + acceptor | - |
Schizophyllum commune | cellotriono-1,5-lactone + reduced acceptor | - |
? | |
1.1.99.18 | cellotriose + acceptor | - |
Schizophyllum commune AS 5.391 | cellotriono-1,5-lactone + reduced acceptor | - |
? | |
1.1.99.18 | lactose + acceptor | - |
Schizophyllum commune | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor | - |
? | |
1.1.99.18 | lactose + acceptor | - |
Schizophyllum commune AS 5.391 | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.99.18 | ? | x * 102000, SDS-PAGE | Schizophyllum commune |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 35 | - |
loss of 40% activity after 8 h at 45°C, enzyme is almost completely inactivated after 4 h at 50° | Schizophyllum commune |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.99.18 | 6 | - |
lactose | - |
Schizophyllum commune | |
1.1.99.18 | 10 | - |
cellopentaose | - |
Schizophyllum commune | |
1.1.99.18 | 12 | - |
cellotriose | - |
Schizophyllum commune | |
1.1.99.18 | 12 | - |
cellotetraose | - |
Schizophyllum commune | |
1.1.99.18 | 13 | - |
cellobiose | - |
Schizophyllum commune | |
1.1.99.18 | 13.5 | - |
ferricyanide | - |
Schizophyllum commune | |
1.1.99.18 | 25 | - |
cytochrome c | - |
Schizophyllum commune | |
1.1.99.18 | 63 | - |
2,6-dichlorphenolindophenol | - |
Schizophyllum commune |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 4.5 | - |
- |
Schizophyllum commune |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.99.18 | 4 | 11 | at 30°C stable for 24 h, at pH 3.5 almost 40% activity are lost within 12 h | Schizophyllum commune |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.99.18 | FAD | - |
Schizophyllum commune | |
1.1.99.18 | heme b | - |
Schizophyllum commune |