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Literature summary extracted from
- Purification and characterization of cellobiose dehydrogenase, a novel extracellular hemoflavoenzyme from the white-rot fungus Phanerochoaete chrysosporium (1993), Arch. Biochem. Biophys., 300, 705-713.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.18 | 0.0012 | - |
cytochrome c | - |
Phanerodontia chrysosporium |  |
| 1.1.99.18 | 0.0036 | - |
2,6-dichlorophenol-indophenol | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.0052 | - |
ferricyanide | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.012 | - |
3,5-di-tert-butyl-1,2-benzoquinone | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.025 | - |
cellobiose | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.055 | - |
cellopentaose | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.061 | - |
cellotetraose | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.062 | - |
cellotriose | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.15 | - |
Mn3+-malonate | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | 0.63 | - |
lactose | - |
Phanerodontia chrysosporium | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.99.18 | extracellular | - |
Phanerodontia chrysosporium | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 90000 | - |
gel filtration | Phanerodontia chrysosporium |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.18 | Phanerodontia chrysosporium | - |
white-rot fungus | - |
| 1.1.99.18 | Phanerodontia chrysosporium OGC 101 | - |
white-rot fungus | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 1.1.99.18 | glycoprotein | 9.4% carbohydrate content | Phanerodontia chrysosporium |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.99.18 | ammonium sulfate, DEAE-Sephadex, Phenyl-Sepharose, Sephacryl S-200, Mono Q | Phanerodontia chrysosporium |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.99.18 | culture medium | - |
Phanerodontia chrysosporium | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 10.3 | - |
- |
Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.18 | cellobiose + acceptor | Cytochrome c, 2,6-dichlorophenolindophenol, ferricyanide, 3,5-di-tert-butyl-1,2-benzoquinone, Mn3+-malonate can serve as electron acceptors. O2 is a poor acceptor in the absence of artificial acceptors and is reduced to H2O2. Molecular oxygen acts as two-electron acceptor, superoxide is produced through an iron mediated pathway | Phanerodontia chrysosporium | cellobiono-1,5-lactone + reduced acceptor | - |
? | |
| 1.1.99.18 | cellobiose + acceptor | Cytochrome c, 2,6-dichlorophenolindophenol, ferricyanide, 3,5-di-tert-butyl-1,2-benzoquinone, Mn3+-malonate can serve as electron acceptors. O2 is a poor acceptor in the absence of artificial acceptors and is reduced to H2O2. Molecular oxygen acts as two-electron acceptor, superoxide is produced through an iron mediated pathway | Phanerodontia chrysosporium OGC 101 | cellobiono-1,5-lactone + reduced acceptor | - |
? | |
| 1.1.99.18 | cellobiose + ferricyanide | - |
Phanerodontia chrysosporium | cellobiono-1,5-lactone + ferrocyanide | - |
r | |
| 1.1.99.18 | cellobiose + Mn(III)-malonate | - |
Phanerodontia chrysosporium | cellobiono-1,5-lactone + reduced Mn(III)-malonate | - |
r | |
| 1.1.99.18 | cellobiose + Mn3+-malonate | - |
Phanerodontia chrysosporium | cellobiono-1,5-lactone + reduced Mn3+-malonate | - |
r | |
| 1.1.99.18 | cellopentaose + acceptor | - |
Phanerodontia chrysosporium | cellopentaono-1,5-lactone + reduced acceptor | - |
? | |
| 1.1.99.18 | cellopentaose + acceptor | - |
Phanerodontia chrysosporium OGC 101 | cellopentaono-1,5-lactone + reduced acceptor | - |
? | |
| 1.1.99.18 | cellotetraose + acceptor | - |
Phanerodontia chrysosporium | cellotetrono-1,5-lactone + reduced acceptor | - |
? | |
| 1.1.99.18 | cellotetraose + acceptor | - |
Phanerodontia chrysosporium OGC 101 | cellotetrono-1,5-lactone + reduced acceptor | - |
? | |
| 1.1.99.18 | cellotriose + acceptor | - |
Phanerodontia chrysosporium | cellotriono-1,5-lactone + reduced acceptor | - |
? | |
| 1.1.99.18 | cellotriose + acceptor | - |
Phanerodontia chrysosporium OGC 101 | cellotriono-1,5-lactone + reduced acceptor | - |
? | |
| 1.1.99.18 | lactose + 3,5-di-tert-butyl-1,2-benzoquinone | - |
Phanerodontia chrysosporium | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + ? | - |
? | |
| 1.1.99.18 | lactose + acceptor | - |
Phanerodontia chrysosporium | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor | - |
? | |
| 1.1.99.18 | lactose + acceptor | - |
Phanerodontia chrysosporium OGC 101 | 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.99.18 | monomer | 1 * 90000, SDS-PAGE | Phanerodontia chrysosporium |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 40 | 60 | 20% activity lost after 12 h at 50°C and pH 6.1, 80% activity lost after 1 h at 60°C and pH 6.1, all activity lost after 10 min at 70°C and pH 6.1 | Phanerodontia chrysosporium |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.18 | 14 | - |
Mn(III)-malonate | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | 20.5 | - |
cytochrome c | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | 20.7 | - |
cellotetraose | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | 21.5 | - |
cellopentaose | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | 22 | - |
ferricyanide | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | 24 | - |
cellobiose | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | 24 | - |
cellotriose | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | 27 | - |
3,5-di-tert-butyl-1,2-benzoquinone | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | 28.8 | - |
lactose | - |
Phanerodontia chrysosporium | |
| 1.1.99.18 | 33 | - |
2,6-dichlorophenol-indophenol | - |
Phanerodontia chrysosporium | |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.18 | 3 | 10.5 | complete inactivation at pH 2 within 2 h | Phanerodontia chrysosporium |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.18 | FAD | hemoflavoenzyme, heme b/FAD/enzyme ratio of 1/1/1 | Phanerodontia chrysosporium | |
| 1.1.99.18 | heme b | hemoflavoenzyme, heme b/FAD/enzyme ratio of 1/1/1 | Phanerodontia chrysosporium | |
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