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Literature summary extracted from

  • Sadana, J.C.; Patil, R.V.
    The purification and properties of cellobiose dehydrogenase from Sclerotium rolfsii and its role in cellulolysis (1985), J. Gen. Microbiol., 131, 1917-1923.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.1.99.18 stable to repeated freezing and thawing Athelia rolfsii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.18 0.0416
-
 cellobiose
-
 Athelia rolfsii
1.1.99.18 0.0625
-
 cellotriose
-
 Athelia rolfsii
1.1.99.18 0.0666
-
 cellotetraose
-
 Athelia rolfsii
1.1.99.18 0.083
-
 cellopentaose
-
 Athelia rolfsii
1.1.99.18 0.1
-
 cellohexaose
-
 Athelia rolfsii
1.1.99.18 0.22
-
 2,6-dichlorophenolindophenol
-
 Athelia rolfsii
1.1.99.18 0.384
-
 cytochrome c
-
 Athelia rolfsii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.18 extracellular bound to mycelium from which it is released into the extracellular medium Athelia rolfsii
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.18 63100
-
 gel permeation Athelia rolfsii
1.1.99.18 64500
-
 1 * 64500, SDS-PAGE Athelia rolfsii

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.18 Athelia rolfsii
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.99.18 glycoprotein 8.9% carbohydrate Athelia rolfsii

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.18
-
 Athelia rolfsii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.99.18 culture filtrate
-
 Athelia rolfsii
-
1.1.99.18 culture medium bound to mycelium from which it is released into the extracellular medium Athelia rolfsii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.18 26.9
-
-
 Athelia rolfsii

Storage Stability

EC Number Storage Stability Organism
1.1.99.18 -15°C, pH 4.5 Athelia rolfsii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.18 cellobiose + acceptor artificial electron acceptors: 2,6-dichlorophenolindophenol, 1,4-benzoquinone, 2,6-dimethyl-1,4-benzoquinone, 2,6-dimethoxy-1,4-benzoquinone, 3,5-di-tert-butyl-1,2-benzoquinone, methylene blue and green, meldola blue, 2,2'-azino-di-(3-ethyl-benzthiazolin-6-sulfonic acid), cytochrome c, K3Fe(CN)6 Athelia rolfsii cellobiono-1,5-lactone + reduced acceptor
-
 ?
1.1.99.18 cellobiose + cytochrome c
-
 Athelia rolfsii cellobiono-1,5-lactone + reduced cytochrome c
-
 r
1.1.99.18 cellohexaose + acceptor
-
 Athelia rolfsii cellohexaono-1,5-lactone + reduced acceptor
-
 ?
1.1.99.18 cellopentaose + acceptor
-
 Athelia rolfsii cellopentaono-1,5-lactone + reduced acceptor
-
 ?
1.1.99.18 cellotetraose + acceptor
-
 Athelia rolfsii cellotetrono-1,5-lactone + reduced acceptor
-
 ?
1.1.99.18 cellotriose + acceptor
-
 Athelia rolfsii cellotriono-1,5-lactone + reduced acceptor
-
 ?
1.1.99.18 lactose + acceptor
-
 Athelia rolfsii 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.18 monomer 1 * 64500, SDS-PAGE Athelia rolfsii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.18 37
-
-
 Athelia rolfsii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.99.18 70
-
 10 min, 86% loss of activity Athelia rolfsii
1.1.99.18 100
-
 2 min, complete loss of activity Athelia rolfsii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.18 6.3 6.4
-
 Athelia rolfsii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.99.18 4.5 5 most stable at Athelia rolfsii