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Literature summary extracted from

  • Molenaar, D.; Van Der Rest, M.E.; Petrovic, S.
    Biochemical and genetic characterization of the membrane-associated malate dehydrogenase (acceptor) from Corynebacterium glutamicum (1998), Eur. J. Biochem., 254, 395-403.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.5.4 2-methyl-1,4-naphthoquinone reduction of 2,6-dichlorophenol indophenol by solubilized enzyme is activated significantly by addition of the quinones decylubiquinone, duroquinone, 2-methyl-1,4-naphthoquinone (vitamin K3), ubiquinone-0 and ubiquinone-1. Optimal activation is observed with ubiquinone-1 Corynebacterium glutamicum
1.1.5.4 decylubiquinone reduction of 2,6-dichlorophenol indophenol by solubilized enzyme is activated significantly by addition of the quinones decylubiquinone, duroquinone, 2-methyl-1,4-naphthoquinone (vitamin K3), ubiquinone-0 and ubiquinone-1. Optimal activation is observed with ubiquinone-1 Corynebacterium glutamicum
1.1.5.4 duroquinone reduction of 2,6-dichlorophenol indophenol by solubilized enzyme is activated significantly by addition of the quinones decylubiquinone, duroquinone, 2-methyl-1,4-naphthoquinone (vitamin K3), ubiquinone-0 and ubiquinone-1. Optimal activation is observed with ubiquinone-1 Corynebacterium glutamicum
1.1.5.4 Lipid activates Corynebacterium glutamicum
1.1.5.4 ubiquinone-0 reduction of 2,6-dichlorophenol indophenol by solubilized enzyme is activated significantly by addition of the quinones decylubiquinone, duroquinone, 2-methyl-1,4-naphthoquinone (vitamin K3), ubiquinone-0 and ubiquinone-1. Optimal activation is observed with ubiquinone-1 Corynebacterium glutamicum
1.1.5.4 ubiquinone-1 reduction of 2,6-dichlorophenol indophenol by solubilized enzyme is activated significantly by addition of the quinones decylubiquinone, duroquinone, 2-methyl-1,4-naphthoquinone (vitamin K3), ubiquinone-0 and ubiquinone-1. Optimal activation is observed with ubiquinone-1 Corynebacterium glutamicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.4
-
 Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.5.4 KSCN
-
 Corynebacterium glutamicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.4 membrane a peripheral membrane protein that can be released from the membrane by addition of chelators Corynebacterium glutamicum 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.4 (S)-malate + acceptor Corynebacterium glutamicum the enzyme takes part in the citric acid cycle. It oxidizes L-malate to oxaloacetate and donates electrons to ubiquinone-1 and other artificial acceptors or, via the electron transfer chain, to oxygen. NAD is not an acceptor and the natural direct acceptor for the enzyme is most likely a quinone. A mutant completely lacking Mqo activity grows poorly on several substrates tested. This enzyme might be especially important when a net flux from malate to oxaloacetate is required, but the intracellular concentrations of the reactants are unfavourable for the NAD-dependent reaction (EC 1.1.1.37) oxaloacetate + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.4 Corynebacterium glutamicum O69282 a mutant completely lacking Mqo activity grows poorly on several substrates tested
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.4 native and His-tagged enzyme Corynebacterium glutamicum

Storage Stability

EC Number Storage Stability Organism
1.1.5.4 when frozen, the activity is stable for several months Corynebacterium glutamicum
1.1.5.4 when stored on ice, the half-life is approximately 120 h, important stabilizing conditions for storage on ice are the presence of EDTA and EGTA. the presence of glycerol, and pH 6. The presence of Mg2+ and Ca2+ has a destabilizing effect Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.4 (S)-malate + 2,6-dichlorophenol indophenol
-
 Corynebacterium glutamicum oxaloacetate + reduced 2,6-dichlorophenol indophenol
-
 ?
1.1.5.4 (S)-malate + acceptor the enzyme takes part in the citric acid cycle. It oxidizes L-malate to oxaloacetate and donates electrons to ubiquinone-1 and other artificial acceptors or, via the electron transfer chain, to oxygen. NAD is not an acceptor and the natural direct acceptor for the enzyme is most likely a quinone. A mutant completely lacking Mqo activity grows poorly on several substrates tested. This enzyme might be especially important when a net flux from malate to oxaloacetate is required, but the intracellular concentrations of the reactants are unfavourable for the NAD-dependent reaction (EC 1.1.1.37) Corynebacterium glutamicum oxaloacetate + reduced acceptor
-
 ?
1.1.5.4 (S)-malate + ubiquinone-1 ubiquinone-1 is directly reduced by the enzyme Corynebacterium glutamicum oxaloacetate + reduced ubiquinone-1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.5.4 malate dehydrogenase (acceptor)
-
 Corynebacterium glutamicum
1.1.5.4 malate:quinone oxidoreductase
-
 Corynebacterium glutamicum
1.1.5.4 Mqo
-
 Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.4 FAD is probably a tightly but non-covalently bound prosthetic group Corynebacterium glutamicum