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Literature summary extracted from

  • Soute, B.M.; Groenen-van Dooren, M.M.; Holmgren, A.; Lundström, J.; Vermeer, C.
    Stimulation of the dithiol-dependent reductases in the vitamin K cycle by the thioredoxin system. Strong synergistic effects with protein disulphide-isomerase (1992), Biochem. J., 281, 255-259.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.4 dithiothreitol
-
 Bos taurus
1.17.4.4 Protein disulfide isomerase activation, together with thioredoxin/thioredoxin reductase and reduced ribonuclease Bos taurus
1.17.4.4 Reduced ribonuclease activation, together with thio-redoxin/thioredoxin reductase, EC 1.6.4.5 plus protein-disulfide isomerase more potent than DTT Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.17.4.4 microsome
-
 Bos taurus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.4.4 2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol Bos taurus
-
 2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.4 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.4.4 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.4.4 0.000009
-
 thioredoxin of bovine thymus Bos taurus
1.17.4.4 0.0000097
-
 thioredoxin from bovine thymus as cofactor, enzyme activity in salt washed detergent-solubilized microsomes Bos taurus
1.17.4.4 0.0000136
-
 thioredoxin from Escherichia coli as cofactor, enzyme activity in salt washed detergent-solubilized microsomes Bos taurus
1.17.4.4 0.0000256
-
 cofactor DTT, enzyme activity in salt washed detergent-solubilized microsomes Bos taurus
1.17.4.4 0.0000498
-
 thioredoxin and protein disulfide-isomerase from bovine thymus as cofactors, enzyme activity in salt washed detergent-solubilized microsomes Bos taurus
1.17.4.4 0.000057
-
 thioredoxin and protein disulfide-isomerase from Escherichia coli as cofactors, enzyme activity in salt washed detergent-solubilized microsomes Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.4 2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol
-
 Bos taurus 2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol
-
 ?
1.17.4.4 vitamin K 2,3-epoxide + dithiothreitol
-
 Bos taurus vitamin K + oxidized dithiothreitol
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.4.4 reduced thioredoxin together with thioredoxin reductase plus protein-disulfide isomerase still more effective than DTT Bos taurus
1.17.4.4 thioredoxin
-
 Bos taurus