EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.6 | 0.02 | - |
cholestanol | - |
Brevibacterium sterolicum | |
1.1.3.6 | 0.07 | - |
cholesterol | - |
Brevibacterium sterolicum | |
1.1.3.6 | 0.11 | - |
cholesterol | - |
Brevibacterium sterolicum | |
1.1.3.6 | 0.165 | - |
O2 | - |
Brevibacterium sterolicum | |
1.1.3.6 | 0.2 | - |
cholesterol | - |
Streptomyces hygroscopicus | |
1.1.3.6 | 0.2 | - |
pregnenolone | - |
Streptomyces hygroscopicus | |
1.1.3.6 | 0.3 | - |
3beta-hydroxy-androst-5-en-17-one | - |
Streptomyces hygroscopicus | |
1.1.3.6 | 0.4 | - |
pregnenolone | - |
Brevibacterium sterolicum | |
1.1.3.6 | 0.5 | - |
3beta-hydroxy-androst-5-en-17-one | - |
Streptomyces hygroscopicus | |
1.1.3.6 | 0.53 | - |
O2 | - |
Streptomyces hygroscopicus | |
1.1.3.6 | 0.7 | - |
cholestanol | - |
Streptomyces hygroscopicus | |
1.1.3.6 | 0.8 | - |
cholesterol | - |
Streptomyces hygroscopicus | |
1.1.3.6 | 0.8 | - |
3beta-hydroxy-androst-5-en-17-one | - |
Brevibacterium sterolicum | |
1.1.3.6 | 1.2 | - |
3beta-hydroxy-androst-5-en-17-one | - |
Brevibacterium sterolicum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.6 | cholesterol + O2 | Streptomyces hygroscopicus | - |
cholest-5-en-3-one + H2O2 | - |
? | |
1.1.3.6 | cholesterol + O2 | Brevibacterium sterolicum | - |
cholest-5-en-3-one + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.6 | Brevibacterium sterolicum | - |
- |
- |
1.1.3.6 | Streptomyces hygroscopicus | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.3.6 | cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Streptomyces hygroscopicus | |
1.1.3.6 | cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Brevibacterium sterolicum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.6 | 1-propanol + O2 | - |
Streptomyces hygroscopicus | propanal + H2O2 | - |
? | |
1.1.3.6 | cholestanol + O2 | - |
Streptomyces hygroscopicus | ? | - |
? | |
1.1.3.6 | cholestanol + O2 | - |
Brevibacterium sterolicum | ? | - |
? | |
1.1.3.6 | cholesterol + O2 | specific for steroid substrates with an OH group in position 3, preference for steroids with at least a C2 chain at position C17 | Streptomyces hygroscopicus | cholest-4-en-3-one + H2O2 | - |
? | |
1.1.3.6 | cholesterol + O2 | specific for steroid substrates with an OH group in position 3, preference for steroids with at least a C2 chain at position C17 | Brevibacterium sterolicum | cholest-4-en-3-one + H2O2 | - |
? | |
1.1.3.6 | cholesterol + O2 | - |
Streptomyces hygroscopicus | cholest-5-en-3-one + H2O2 | - |
? | |
1.1.3.6 | cholesterol + O2 | - |
Brevibacterium sterolicum | cholest-5-en-3-one + H2O2 | - |
? | |
1.1.3.6 | cyclohexanol + O2 | - |
Streptomyces hygroscopicus | cyclohexanal + H2O2 | - |
? | |
1.1.3.6 | epiandrosterone + O2 | - |
Streptomyces hygroscopicus | ? | - |
? | |
1.1.3.6 | ethanol + O2 | - |
Streptomyces hygroscopicus | ethanal + H2O2 | - |
? | |
1.1.3.6 | methanol + O2 | - |
Streptomyces hygroscopicus | methanal + H2O2 | - |
? | |
1.1.3.6 | pregnenolone + O2 | - |
Streptomyces hygroscopicus | ? | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.6 | 0.8 | - |
3beta-hydroxy-androst-5-en-17-one | product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer | Brevibacterium sterolicum | |
1.1.3.6 | 0.8 | - |
3beta-hydroxy-5-androsten-17-one | H2O2 production assayed, 100 mM phosphate buffer | Brevibacterium sterolicum | |
1.1.3.6 | 7 | - |
epiandrosterone | H2O2 production assayed, 100 mM phosphate buffer | Streptomyces hygroscopicus | |
1.1.3.6 | 8.2 | - |
3beta-hydroxy-androst-5-en-17-one | product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer | Streptomyces hygroscopicus | |
1.1.3.6 | 21 | - |
pregnenolone | product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer | Brevibacterium sterolicum | |
1.1.3.6 | 24 | - |
pregnenolone | product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer | Streptomyces hygroscopicus | |
1.1.3.6 | 28 | - |
cholesterol | 50 mM phosphate buffer, pH 7.5 | Streptomyces hygroscopicus | |
1.1.3.6 | 37 | - |
cholestanol | H2O2 production assayed, 100 mM phosphate buffer | Streptomyces hygroscopicus | |
1.1.3.6 | 40 | - |
cholestanol | H2O2 production assayed, 100 mM phosphate buffer | Brevibacterium sterolicum | |
1.1.3.6 | 48 | - |
cholesterol | 50 mM phosphate buffer, pH 7.5 | Brevibacterium sterolicum | |
1.1.3.6 | 48.1 | - |
cholesterol | product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer | Brevibacterium sterolicum | |
1.1.3.6 | 63 | - |
cholesterol | product formation followed at 240 nm in the presence of 0.02 ml H2O2, substrate dissolved in Triton X-100, 100 mM phosphate buffer | Streptomyces hygroscopicus | |
1.1.3.6 | 105 | - |
cholesterol | 50 mM phosphate buffer, pH 7.5, 1% thesit, 10% 2-propanol | Brevibacterium sterolicum | |
1.1.3.6 | 250 | - |
5-Cholesten-3-one | isomerization to 4-cholesten-3-one, rapid reaction, stopped-flow measurement, 500 mM phosphate buffer, pH 7.5, in the presence of 1% thesit and 1.25% 2-propanol | Brevibacterium sterolicum | |
1.1.3.6 | 330 | - |
5-Cholesten-3-one | isomerization to 4-cholesten-3-one, rapid reaction, stopped-flow measurement, 500 mM phosphate buffer, pH 7.5, in the presence of 1% thesit and 1.25% 2-propanol | Streptomyces hygroscopicus | |
1.1.3.6 | 345 | - |
cholesterol | 50 mM phosphate buffer, pH 7.5, 1% thesit, 10% 2-propanol | Streptomyces hygroscopicus | |
1.1.3.6 | 670 | - |
5-Cholesten-3-one | isomerization to 4-cholesten-3-one, rapid reaction, stopped-flow measurement, 50 mM phosphate buffer, pH 7.5, in the presence of 1% thesit and 1.25% 2-propanol | Streptomyces hygroscopicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.6 | FAD | non-covalently bound | Streptomyces hygroscopicus | |
1.1.3.6 | FAD | covalently bound in His-tagged recombinant enzyme | Brevibacterium sterolicum |