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Literature summary extracted from

  • Liu, W.H.; Meng, M.H.; Chen, K.S.
    Purification and some properties of cholesterol oxidases produced by an inducible and a constitutive mutant of Arthrobacter simplex (1988), Agric. Biol. Chem., 52, 413-418.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.6 Triton X-100 stimulates at concentration up to 0.2% Pimelobacter simplex

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.6 HgCl2
-
 Pimelobacter simplex
1.1.3.6 sulfhydryl reagents
-
 Pimelobacter simplex
1.1.3.6 Triton X-100 stimulates at concentration up to 0.2% Pimelobacter simplex

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.6 cholesterol + O2 Pimelobacter simplex
-
 cholest-5-en-3-one + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 Pimelobacter simplex
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.6 inducible and constitutive enzyme Pimelobacter simplex

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.6 cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Pimelobacter simplex
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.6 cell culture
-
 Pimelobacter simplex
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.6 2.42
-
 inducible enzyme Pimelobacter simplex
1.1.3.6 3.65
-
 constitutive enzyme Pimelobacter simplex

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 cholesterol + O2
-
 Pimelobacter simplex cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Pimelobacter simplex cholest-5-en-3-one + H2O2
-
 ?
1.1.3.6 ergosterol + O2 low activity Pimelobacter simplex dehydroergosterol + H2O2
-
 ?
1.1.3.6 stigmasterol + O2
-
 Pimelobacter simplex stigmasta-5,22-dien-3-one + H2O2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.6 50
-
-
 Pimelobacter simplex

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.6 33 60 constitutive enzyme, 50% of maximal activity at 33°C, 70% at 60°C, inducible enzyme, 50% of maximal activity at 33°C and 60°C Pimelobacter simplex

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.6 7.5
-
-
 Pimelobacter simplex

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.6 6 9.5 inducible enzyme, 80% of maximal activity at pH 6.0, 50% at pH 9.5, constitutive enzyme, 70% of maximal activity at pH 6.0, 50% at pH 9.5 Pimelobacter simplex

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.6 6 10 30°C, 2 h, stable Pimelobacter simplex