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Literature summary extracted from

  • Groen, B.W.; de Vries, S.; Duine, J.A.
    Characterization of hexose oxidase from the red seaweed Chondrus crispus (1997), Eur. J. Biochem., 244, 858-861.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.5 ethanol 100 mM, 1.5fold increase in activity Chondrus crispus
1.1.3.5 NaCl 20 mM, 1.4fold increase in activity Chondrus crispus

General Stability

EC Number General Stability Organism
1.1.3.5 very stable enzyme, no activity decrease after storage at room temperature overnight Chondrus crispus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.5 0.3
-
 O2
-
 Chondrus crispus
1.1.3.5 1.7
-
 lactose
-
 Chondrus crispus
1.1.3.5 8.5
-
 D-glucose
-
 Chondrus crispus
1.1.3.5 12.5
-
 cellobiose
-
 Chondrus crispus
1.1.3.5 20
-
 D-galactose
-
 Chondrus crispus
1.1.3.5 28
-
 maltose
-
 Chondrus crispus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.5 87000
-
 native PAGE on gradient gel Chondrus crispus

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.5 Chondrus crispus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.3.5 glycoprotein
-
 Chondrus crispus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.5 Q-Sepharose, Phenyl-sepharose, Superdex 200 Chondrus crispus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.5 120
-
-
 Chondrus crispus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.5 cellobiose + O2
-
 Chondrus crispus 4-O-(beta-D-glucopyranosido)-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.5 D-galactose + O2
-
 Chondrus crispus D-galactono-1,5-lactone + H2O2
-
 ?
1.1.3.5 D-glucose + O2
-
 Chondrus crispus D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.5 lactose + O2
-
 Chondrus crispus 4-O-(beta-D-galactopyranosido)-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.5 maltose + O2
-
 Chondrus crispus 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + H2O2
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.5 6
-
 broad pH range, two optima can be distinguished Chondrus crispus