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Literature summary extracted from

  • Sullivan jr., J.D.; Ikawa, M.
    Purification and characterization of hexose oxidase from the red alga Chondrus crispus (1973), Biochim. Biophys. Acta, 309, 11-22.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.5 acetate
-
 Chondrus crispus
1.1.3.5 azide
-
 Chondrus crispus
1.1.3.5 cyanide
-
 Chondrus crispus
1.1.3.5 diethyldithiocarbamate
-
 Chondrus crispus
1.1.3.5 hydroxylamine
-
 Chondrus crispus
1.1.3.5 pyruvate
-
 Chondrus crispus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.5 4
-
 D-glucose
-
 Chondrus crispus
1.1.3.5 8
-
 D-galactose
-
 Chondrus crispus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.5 Cu2+ 12 mol per mol enzyme Chondrus crispus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.5 130000
-
-
 Chondrus crispus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.5 D-glucose + O2 Chondrus crispus
-
 D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.5 D-glucose + O2 Euthora cristata
-
 D-glucono-1,5-lactone + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.5 Chondrus crispus
-
 irish moss, red alga, red seaweed
-
1.1.3.5 Euthora cristata
-
 red alga
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.3.5 glycoprotein 70% carbohydrates, mainly galactose and xylose Chondrus crispus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.5
-
 Chondrus crispus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.5 4.095
-
-
 Chondrus crispus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.5 2-deoxy-D-galactose + O2 6% of activity with D-glucose Chondrus crispus 2-deoxy-D-galactono-1,5-lactone + H2O2
-
 ?
1.1.3.5 2-deoxy-D-glucose + O2 8% of activity with D-glucose Chondrus crispus 2-deoxy-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.5 cellobiose + O2
-
 Chondrus crispus 4-O-(beta-D-glucopyranosido)-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.5 cellobiose + O2
-
 Euthora cristata 4-O-(beta-D-glucopyranosido)-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.5 D-galactose + O2
-
 Euthora cristata D-galactono-1,5-lactone + H2O2
-
 ?
1.1.3.5 D-galactose + O2 82% of activity with D-glucose Chondrus crispus D-galactono-1,5-lactone + H2O2
-
 ?
1.1.3.5 D-glucose + O2
-
 Chondrus crispus D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.5 D-glucose + O2
-
 Euthora cristata D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.5 D-glucose-6-phosphate + O2 10% of activity with D-glucose Chondrus crispus D-glucono-1,5-lactone-6-phosphate + H2O2
-
 ?
1.1.3.5 lactose + O2
-
 Chondrus crispus 4-O-(beta-D-galactopyranosido)-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.5 lactose + O2
-
 Euthora cristata 4-O-(beta-D-galactopyranosido)-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.5 maltose + O2
-
 Euthora cristata 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.5 maltose + O2 40% of activity with D-glucose Chondrus crispus 4-O-(alpha-D-glucopyranosido)-D-glucono-1,5-lactone + H2O2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.5 25
-
-
 Chondrus crispus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.5 50 60 not stable above Chondrus crispus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.5 6.3
-
-
 Chondrus crispus