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Literature summary extracted from

  • Kawagoshi, Y.; Fujita, M.
    Purification and properties of polyvinyl alcohol oxidase with broad substrate range obtained from Pseudomonas vesicularis var. povalolyticus PH (1997), World J. Microbiol. Biotechnol., 13, 273-277.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.18 Cu2+ weak inhibition Brevundimonas vesicularis
1.1.3.18 EDTA weak inhibition Brevundimonas vesicularis
1.1.3.18 EDTA weak inhibition Pseudomonas sp.
1.1.3.18 Fe2+ strongly inhibited by Brevundimonas vesicularis
1.1.3.18 Hg2+
-
 Brevundimonas vesicularis
1.1.3.18 Hg2+
-
 Pseudomonas sp.
1.1.3.18 iodoacetamide
-
 Brevundimonas vesicularis
1.1.3.18 Sn2+ strongly inhibited by Brevundimonas vesicularis
1.1.3.18 Thiourea
-
 Brevundimonas vesicularis
1.1.3.30 EDTA 1 mM, 23% inhibition Brevundimonas vesicularis
1.1.3.30 Fe2+ 1 mM, 88% inhibition Brevundimonas vesicularis
1.1.3.30 Hg2+ 1 mM, 93% inhibition Brevundimonas vesicularis
1.1.3.30 Sn2+ 1 mM, 89% inhibition Brevundimonas vesicularis
1.1.3.30 Thiourea 1 mM, 20% inhibition Brevundimonas vesicularis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.18 extracellular
-
 Pseudomonas sp.
-
-
1.1.3.18 extracellular
-
 Brevundimonas vesicularis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.18 Ca2+ relative activity 106% Brevundimonas vesicularis
1.1.3.18 Fe2+ contains 1 atom non-heme iron per molecule, catalyzes the decomposition of H2O2 Pseudomonas sp.
1.1.3.18 Fe2+ contains 1 atom non-heme iron per molecule, catalyzes the decomposition of H2O2 Brevundimonas vesicularis
1.1.3.18 Mg2+ relative activity 103% Brevundimonas vesicularis
1.1.3.30 Fe2+ 1 mol iron/mol enzyme Brevundimonas vesicularis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.18 75000
-
 gel filtration Brevundimonas vesicularis
1.1.3.18 85000
-
 SDS-PAGE Brevundimonas vesicularis
1.1.3.30 75000
-
 gel filtration Brevundimonas vesicularis
1.1.3.30 85000
-
 1 * 85000, SDS-PAGE Brevundimonas vesicularis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.30 polyvinyl alcohol + O2 Brevundimonas vesicularis
-
 oxidized polyvinyl alcohol + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.18 Brevundimonas vesicularis
-
 var. povalolyticus PH
-
1.1.3.18 Pseudomonas sp.
-
-
-
1.1.3.18 Pseudomonas sp.
-
 O-3
-
1.1.3.18 Pseudomonas sp. O-3
-
 O-3
-
1.1.3.30 Brevundimonas vesicularis
-
 var. povalolyticus PH
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.18
-
 Pseudomonas sp.
1.1.3.18
-
 Brevundimonas vesicularis
1.1.3.30 Q-Sepharose, AF blue Toyopearl, Q-Sepharose Brevundimonas vesicularis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.18 0.7
-
-
 Brevundimonas vesicularis
1.1.3.30 0.7
-
-
 Brevundimonas vesicularis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.18 1,2-propanediol + O2
-
 Pseudomonas sp. 1,2-propanedione + H2O2
-
 ?
1.1.3.18 1,2-propanediol + O2
-
 Brevundimonas vesicularis 1,2-propanedione + H2O2
-
 ?
1.1.3.18 1,3-butanediol + O2
-
 Brevundimonas vesicularis 1,3-butanedione + H2O2
-
 ?
1.1.3.18 1,4-butanediol + O2
-
 Brevundimonas vesicularis 1,4-butanedione + H2O2
-
 ?
1.1.3.18 1-butanol + O2
-
 Pseudomonas sp. n-butanal + H2O2
-
 ?
1.1.3.18 1-butanol + O2
-
 Brevundimonas vesicularis n-butanal + H2O2
-
 ?
1.1.3.18 2,4-pentanediol + O2
-
 Brevundimonas vesicularis 2,4-pentanedione + H2O2
-
 ?
1.1.3.18 2,5-hexanediol + O2
-
 Pseudomonas sp. 2,5-hexanedione + H2O2
-
 ?
1.1.3.18 2,5-hexanediol + O2
-
 Brevundimonas vesicularis 2,5-hexanedione + H2O2
-
 ?
1.1.3.18 2,5-hexanediol + O2
-
 Pseudomonas sp. O-3 2,5-hexanedione + H2O2
-
 ?
1.1.3.18 2-butanol + O2
-
 Pseudomonas sp. 2-butanone + H2O2
-
 ?
1.1.3.18 2-butanol + O2
-
 Brevundimonas vesicularis 2-butanone + H2O2
-
 ?
1.1.3.18 2-heptanol + O2
-
 Pseudomonas sp. 2-heptanone + H2O2
-
 ?
1.1.3.18 2-heptanol + O2
-
 Brevundimonas vesicularis 2-heptanone + H2O2
-
 ?
1.1.3.18 2-hexanol + O2
-
 Pseudomonas sp. 2-hexanone + H2O2
-
 ?
1.1.3.18 2-hexanol + O2
-
 Brevundimonas vesicularis 2-hexanone + H2O2
-
 ?
1.1.3.18 2-hexanol + O2
-
 Pseudomonas sp. O-3 2-hexanone + H2O2
-
 ?
1.1.3.18 2-octanol + O2
-
 Pseudomonas sp. 2-octanone + H2O2
-
 ?
1.1.3.18 2-octanol + O2
-
 Brevundimonas vesicularis 2-octanone + H2O2
-
 ?
1.1.3.18 2-pentanol + O2
-
 Pseudomonas sp. 2-pentanone + H2O2
-
 ?
1.1.3.18 2-pentanol + O2
-
 Brevundimonas vesicularis 2-pentanone + H2O2
-
 ?
1.1.3.18 2-pentanol + O2
-
 Pseudomonas sp. O-3 2-pentanone + H2O2
-
 ?
1.1.3.18 2-propanol + O2
-
 Pseudomonas sp. 2-propanone + H2O2
-
 ?
1.1.3.18 2-propanol + O2
-
 Brevundimonas vesicularis 2-propanone + H2O2
-
 ?
1.1.3.18 3-heptanol + O2
-
 Pseudomonas sp. 3-heptanone + H2O2
-
 ?
1.1.3.18 3-heptanol + O2
-
 Brevundimonas vesicularis 3-heptanone + H2O2
-
 ?
1.1.3.18 3-hexanol + O2
-
 Pseudomonas sp. 3-hexanone + H2O2
-
 ?
1.1.3.18 3-hexanol + O2
-
 Brevundimonas vesicularis 3-hexanone + H2O2
-
 ?
1.1.3.18 3-pentanol + O2
-
 Pseudomonas sp. 3-pentanone + H2O2
-
 ?
1.1.3.18 3-pentanol + O2
-
 Brevundimonas vesicularis 3-pentanone + H2O2
-
 ?
1.1.3.18 4-decanol + O2
-
 Pseudomonas sp. 4-decanone + H2O2
-
 ?
1.1.3.18 4-decanol + O2
-
 Brevundimonas vesicularis 4-decanone + H2O2
-
 ?
1.1.3.18 4-decanol + O2
-
 Pseudomonas sp. O-3 4-decanone + H2O2
-
 ?
1.1.3.18 4-heptanol + O2
-
 Pseudomonas sp. 4-heptanone + H2O2
-
 ?
1.1.3.18 4-heptanol + O2
-
 Brevundimonas vesicularis 4-heptanone + H2O2
-
 ?
1.1.3.18 4-heptanol + O2
-
 Pseudomonas sp. O-3 4-heptanone + H2O2
-
 ?
1.1.3.18 benzyl alcohol + O2
-
 Pseudomonas sp. benzaldehyde + H2O2
-
 ?
1.1.3.18 benzyl alcohol + O2
-
 Brevundimonas vesicularis benzaldehyde + H2O2
-
 ?
1.1.3.18 catechol + O2
-
 Brevundimonas vesicularis o-benzoquinone + H2O2
-
 ?
1.1.3.18 cyclohexanol + O2
-
 Pseudomonas sp. cyclohexanone + H2O2
-
 ?
1.1.3.18 cyclohexanol + O2
-
 Brevundimonas vesicularis cyclohexanone + H2O2
-
 ?
1.1.3.18 l-menthol + O2
-
 Brevundimonas vesicularis menthone + H2O2
-
 ?
1.1.3.18 polyvinyl alcohol + O2
-
 Pseudomonas sp. polyvinylketone + H2O2
-
 ?
1.1.3.18 polyvinyl alcohol + O2
-
 Brevundimonas vesicularis polyvinylketone + H2O2
-
 ?
1.1.3.30 1,3-butanediol + O2 3.8% of activity with polyvinyl alcohol Brevundimonas vesicularis 1,3-butanedione + H2O2
-
 ?
1.1.3.30 2,4-pentanediol + O2 19.3% of activity with polyvinyl alcohol Brevundimonas vesicularis 2,4-pentanedione + H2O2
-
 ?
1.1.3.30 2,5-hexanediol + O2 3.8% of activity with polyvinyl alcohol Brevundimonas vesicularis 2,5-hexanedione + H2O2
-
 ?
1.1.3.30 2-butanol + O2 47.3% of activity with polyvinyl alcohol Brevundimonas vesicularis 2-butanone + H2O2
-
 ?
1.1.3.30 2-hexanol + O2 65.4% of activity with polyvinyl alcohol Brevundimonas vesicularis 2-hexanone + H2O2
-
 ?
1.1.3.30 2-pentanol + O2 36.3% of activity with polyvinyl alcohol Brevundimonas vesicularis 2-pentanone + H2O2
-
 ?
1.1.3.30 2-propanol + O2 3.5% of activity with polyvinyl alcohol Brevundimonas vesicularis acetone + H2O2
-
 ?
1.1.3.30 3-butanol + O2 14.6% of activity with polyvinyl alcohol Brevundimonas vesicularis 3-butanone + H2O2
-
 ?
1.1.3.30 3-heptanol + O2 68.8% of activity with polyvinyl alcohol Brevundimonas vesicularis 3-heptanone + H2O2
-
 ?
1.1.3.30 3-hexanol + O2 47.3% of activity with polyvinyl alcohol Brevundimonas vesicularis 3-hexanone + H2O2
-
 ?
1.1.3.30 4-decanol + O2 100% of activity with polyvinyl alcohol Brevundimonas vesicularis 4-decanone + H2O2
-
 ?
1.1.3.30 4-heptanol + O2 75.1% of activity with polyvinyl alcohol Brevundimonas vesicularis 4-heptanone + H2O2
-
 ?
1.1.3.30 benzyl alcohol + O2 13.7% of activity with polyvinyl alcohol Brevundimonas vesicularis benzaldehyde + H2O2
-
 ?
1.1.3.30 cyclohexanol + O2 41.8% of activity with polyvinyl alcohol Brevundimonas vesicularis cyclohexanal + H2O2
-
 ?
1.1.3.30 polyvinyl alcohol + O2
-
 Brevundimonas vesicularis oxidized polyvinyl alcohol + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.18 monomer
-
 Pseudomonas sp.
1.1.3.18 monomer 1 * 85000, SDS-PAGE Brevundimonas vesicularis
1.1.3.30 monomer 1 * 85000, SDS-PAGE Brevundimonas vesicularis

Synonyms

EC Number Synonyms Comment Organism
1.1.3.18 PVA oxidase
-
 Brevundimonas vesicularis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.18 40
-
-
 Pseudomonas sp.
1.1.3.18 40
-
-
 Brevundimonas vesicularis
1.1.3.30 40
-
-
 Brevundimonas vesicularis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.30 35 45
-
 Brevundimonas vesicularis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.18 45
-
 stable up to Brevundimonas vesicularis
1.1.3.30 45
-
 stable for 30 min, complete loss of activity after 30 min at 60°C Brevundimonas vesicularis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.18 10
-
-
 Brevundimonas vesicularis
1.1.3.30 10
-
-
 Brevundimonas vesicularis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.30 7 11 almost constant activity in this range Brevundimonas vesicularis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.18 6 10 stable between Brevundimonas vesicularis
1.1.3.30 6 10 stable for at least 24 h at 30°C Brevundimonas vesicularis