EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.95 | - |
Escherichia coli |
EC Number | General Stability | Organism |
---|---|---|
1.1.1.95 | dithiothreitol increases stability | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.95 | Ag+ | inhibition can be overcome by addition of dithiothreitol | Escherichia coli | |
1.1.1.95 | L-serine | inhibition of enzyme from E. coli, Salmonella typhimurium and Haemophilus influenzae, not of mammalian enzyme, inhibition in both reaction directions | Escherichia coli | |
1.1.1.95 | Mercurials | inhibition can be overcome by addition of dithiothreitol | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.95 | 0.0013 | - |
3-phosphohydroxypyruvate | Km at pH 7.5 is lower than at pH 8.8 | Escherichia coli | |
1.1.1.95 | 0.0078 | - |
NAD+ | Km at pH 7.5 is lower than at pH 8.8 | Escherichia coli | |
1.1.1.95 | 1.1 | - |
D-3-phosphoglycerate | - |
Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.95 | 163000 | - |
sedimentation equilibrium analysis | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.95 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.95 | - |
Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.95 | additional information | - |
- |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.95 | 3-phospho-D-glycerate + NAD+ | - |
Escherichia coli | 3-phosphohydroxypyruvate + NADH | - |
? | |
1.1.1.95 | 3-phosphoglycerate + NAD+ | - |
Escherichia coli | 3-phosphohydroxypyruvate + NADH | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.95 | tetramer | 4 * 40000-50000, sedimentation equilibrium in guanidine-HCl | Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.95 | 25 | - |
assay at | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.95 | 8.5 | - |
initial rate maximum, but enzyme not stable | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.95 | 3-acetylpyridine-NADH | 40% as effective as NADH | Escherichia coli | |
1.1.1.95 | deamino-NADH | as effective as NADH | Escherichia coli | |
1.1.1.95 | NAD+ | - |
Escherichia coli | |
1.1.1.95 | NADH | - |
Escherichia coli | |
1.1.1.95 | NADPH | 8% as effective as NADH | Escherichia coli |