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Literature summary extracted from

  • Kito, M.; Pizer, L.I.
    Purification and regulatory properties of the biosynthetic L-glycerol 3-phosphate dehydrogenase from Escherichia coli (1969), J. Biol. Chem., 244, 3316-3323.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.94 (NH4)2SO4 100 mM, 68% inhibition, 10 mM, 8% inhibition Escherichia coli
1.1.1.94 DL-glycerol 3-phosphate 0.05 mM, 26% inhibition Escherichia coli
1.1.1.94 glycerol 3-phosphate 50% inhibition with 0.035 mM, sigmoid inhibition curve, competitive inhibition vs. glycerone phosphate, uncompetitive vs. NADPH Escherichia coli
1.1.1.94 K2PO4- 100 mM, 95% inhibition, 10 mM, 27% inhibition Escherichia coli
1.1.1.94 K2SO4 100 mM, 60% inhibition Escherichia coli
1.1.1.94 KCl 100 mM, 47% inhibition Escherichia coli
1.1.1.94 Na2PO4- 100 mM, 92% inhibition, 10 mM, 27% inhibition Escherichia coli
1.1.1.94 Na2SO4 100 mM, 63% inhibition Escherichia coli
1.1.1.94 NaCl 100 mM, 47% inhibition Escherichia coli
1.1.1.94 NH4Cl 100 mM, 37% inhibition Escherichia coli
1.1.1.94 palmitoyl-CoA 0.0015 mM, 52% inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.94 0.01
-
 NADPH
-
 Escherichia coli
1.1.1.94 0.17
-
 glycerone phosphate
-
 Escherichia coli
1.1.1.94 0.21
-
 glycerol-3-phosphate
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.94 sn-glycerol 3-phosphate + NAD(P)+ Escherichia coli
-
 glycerone phosphate + NAD(P)H + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.94 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.94 streptomycin, ammonium sulfate, DEAE-Sephadex, Sephadex G-150, DEAE-Sephadex Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.94 4800
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.94 sn-glycerol 3-phosphate + NAD(P)+
-
 Escherichia coli glycerone phosphate + NAD(P)H + H+
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.94 7.4
-
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.94 deamino-NAD+ 2fold activity compared to NAD+ Escherichia coli
1.1.1.94 NAD+
-
 Escherichia coli
1.1.1.94 NADP+ 2fold activity compared to NAD+ Escherichia coli