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Literature summary extracted from

  • Kohn, L.D.; Jakoby W., B.
    Tartaric acid metabolism. VI. Crystalline oxaloglcolate reductive decarboxylase (1968), J. Biol. Chem., 243, 2486-2493.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.92 0.015
-
 NADPH 2 mM 2,3-dihydroxysuccinate as substrate Pseudomonas putida
1.1.1.92 0.024
-
 NADPH 2 mM hydroxypyruvate as substrate Pseudomonas putida
1.1.1.92 0.14
-
 NADH 2 mM 2,3-dihydroxysuccinate as substrate Pseudomonas putida
1.1.1.92 0.17
-
 NADH 2 mM hydroxypyruvate as substrate Pseudomonas putida
1.1.1.92 1
-
 Hydroxypyruvate cofactor NADH Pseudomonas putida
1.1.1.92 1.2
-
 2,3-dihydroxysuccinate cofactor NADH Pseudomonas putida
1.1.1.92 2.5
-
 Hydroxypyruvate cofactor NADPH Pseudomonas putida
1.1.1.92 2.5
-
 2,3-dihydroxysuccinate cofactor NADPH Pseudomonas putida
1.1.1.92 3.3
-
 glyoxylate cofactor NADH Pseudomonas putida
1.1.1.92 8
-
 glyoxylate cofactor NADPH Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.92 63000
-
 sedimentation equilibrium Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.92 2-hydroxy-3-oxosuccinate + NAD(P)H Pseudomonas putida
-
 D-glycerate + NAD(P)+ + CO2
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.92 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.92 protamine sulfate, ammonium sulfate, DEAE-cellulose, Sephadex G-100, disc electrophoresis, Sephadex G-100, crystallization Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.92 2.6
-
 substrate 2,3-dihydroxy-succinate Pseudomonas putida
1.1.1.92 7.7
-
 substrate hydroxypyruvate Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.1.1.92 -15°C, 40 mM Tris-HCl, 30% glycerol, 5 mM mercaptoethanol, 6 months, 10% loss of activity Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.92 2,3-dihydroxysuccinate + NAD(P)H
-
 Pseudomonas putida D-glycerate + NAD(P)+ + CO2
-
 ir
1.1.1.92 2-hydroxy-3-oxosuccinate + NAD(P)H
-
 Pseudomonas putida D-glycerate + NAD(P)+ + CO2
-
 r
1.1.1.92 2-hydroxy-3-oxosuccinate + NAD(P)H enzyme is inhibited at higher levels of ionic strength Pseudomonas putida D-glycerate + NAD(P)+ + CO2
-
 r
1.1.1.92 2-hydroxy-3-oxosuccinate + NADH + H+
-
 Pseudomonas putida D-glycerate + NAD+ + CO2
-
 r
1.1.1.92 glyoxylate + NAD(P)H
-
 Pseudomonas putida glycolic acid + NAD(P)+
-
 ir
1.1.1.92 hydroxypyruvate + NAD(P)H
-
 Pseudomonas putida D-glycerate + NAD(P)+ + H+
-
 ir

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.92 6
-
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.92 NAD(P)H
-
 Pseudomonas putida