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Literature summary extracted from

  • Rogers, K.S.; Rodwell, V.W.; Geiger, P.
    Active form of Pseudomonas mevalonii 3-hydroxy-3-methylglutaryl coenzyme A reductase (1997), Biochem. Mol. Med., 61, 114-120.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.88 medicine target enzyme for chemotherapy of hypercholesterolemias Pseudomonas mevalonii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.88 wild-type and mutants, H381A, H381N, H381K, H381Q, 3 A resolution Pseudomonas mevalonii

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.88 H381K
-
 Pseudomonas mevalonii
1.1.1.88 H381N
-
 Pseudomonas mevalonii
1.1.1.88 H381Q
-
 Pseudomonas mevalonii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.88 45540
-
 amino acid composition Pseudomonas mevalonii
1.1.1.88 270100
-
 sedimentation equilibrium centrifugation Pseudomonas mevalonii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.88 (S)-3-hydroxy-3-methylglutaryl-CoA + NADH + H+ Pseudomonas mevalonii
-
 (R)-mevalonate + NAD+ + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.88 Pseudomonas mevalonii
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.88 (R)-mevalonate + CoA + 2 NAD+ = 3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+ reaction mechanism purposal Pseudomonas mevalonii
a

Storage Stability

EC Number Storage Stability Organism
1.1.1.88 -180°C, 400 mM KCl, 24 months Pseudomonas mevalonii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.88 (R)-mevalonate + CoA + 2 NAD+
-
 Pseudomonas mevalonii (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+
-
 ?
1.1.1.88 (S)-3-hydroxy-3-methylglutaryl-CoA + NADH + H+
-
 Pseudomonas mevalonii (R)-mevalonate + NAD+ + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.88 hexamer 3alpha2, XSA Pseudomonas mevalonii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.88 NAD+
-
 Pseudomonas mevalonii
1.1.1.88 NADH reverse reaction Pseudomonas mevalonii