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Literature summary extracted from

  • Darnay, B.G.; Wang, Y.; Rodwell, V.W.
    Identification of the catalytically important histidine of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (1992), J. Biol. Chem., 267, 15064-15070.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.88 expressed in Escherichia coli BL21 Pseudomonas mevalonii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.88 wild-type and mutants, H381A, H381N, H381K, H381Q, 3 A resolution Pseudomonas mevalonii

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.88 H381A 6% of wild-type activity Pseudomonas mevalonii
1.1.1.88 H381K 45% of wild-type activity at pH 11 Pseudomonas mevalonii
1.1.1.88 H381N 16% of wild-type activity Pseudomonas mevalonii
1.1.1.88 H381Q 23% of wild-type activity Pseudomonas mevalonii

General Stability

EC Number General Stability Organism
1.1.1.88 diethyl polycarbonate causes loss of activity Pseudomonas mevalonii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.88 0.03
-
 CoASH H381A mutant, oxidative acylation of mevalonate Pseudomonas mevalonii
1.1.1.88 0.05
-
 CoASH wild-type enzyme, oxidative acylation of mevalonate Pseudomonas mevalonii
1.1.1.88 0.11
-
 CoASH H381Q mutant, oxidative acylation of mevalonate Pseudomonas mevalonii
1.1.1.88 0.14
-
 NAD+ H381A mutant, oxidative acylation of mevalonate Pseudomonas mevalonii
1.1.1.88 0.2
-
 CoASH H381N mutant, oxidative acylation of mevalonate Pseudomonas mevalonii
1.1.1.88 0.22
-
 NAD+ wild-type enzyme, oxidative acylation of mevalonate Pseudomonas mevalonii
1.1.1.88 0.27
-
 (R,S)-mevalonate H381A mutant, oxidative acylation of mevalonate Pseudomonas mevalonii
1.1.1.88 0.45
-
 (R,S)-mevalonate wild-type enzyme, oxidative acylation of mevalonate Pseudomonas mevalonii
1.1.1.88 0.5
-
 CoASH H381K mutant, oxidative acylation of mevalonate Pseudomonas mevalonii
1.1.1.88 0.62
-
 NAD+ H381Q mutant, oxidative acylation of mevalonate Pseudomonas mevalonii
1.1.1.88 0.71
-
 (R,S)-mevalonate H381Q mutant, oxidative acylation of mevalonate Pseudomonas mevalonii
1.1.1.88 0.75
-
 (R,S)-mevalonate H381K mutant, oxidative acylation of mevalonate Pseudomonas mevalonii
1.1.1.88 0.81
-
 NAD+ H381N mutant, oxidative acylation of mevalonate Pseudomonas mevalonii
1.1.1.88 1.23
-
 NAD+ H381K mutant, oxidative acylation of mevalonate Pseudomonas mevalonii
1.1.1.88 3.7
-
 (R,S)-mevalonate H381N mutant, oxidative acylation of mevalonate Pseudomonas mevalonii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.88 Pseudomonas mevalonii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.88
-
 Pseudomonas mevalonii

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.1.1.88 after inactivation with diethyl polycarbonate addition of hydroxylamine restores 50% of activity Pseudomonas mevalonii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.88 0.011
-
 H381A-mutant, mevalonate acylation Pseudomonas mevalonii
1.1.1.88 0.029
-
 H381N-mutant, mevalonate acylation Pseudomonas mevalonii
1.1.1.88 0.041
-
 H381Q-mutant, mevalonate acylation Pseudomonas mevalonii
1.1.1.88 0.091
-
 H381K-mutant, mevalonate acylation Pseudomonas mevalonii
1.1.1.88 18
-
 mevalonate acylation pH 8.1 Pseudomonas mevalonii
1.1.1.88 20.3
-
 mevalonate acylation pH 11 Pseudomonas mevalonii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.88 (R)-mevalonate + CoA + 2 NAD+
-
 Pseudomonas mevalonii (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+
-
 ?
1.1.1.88 3-hydroxy-3-methylglutaryl-CoA + NADH + H+
-
 Pseudomonas mevalonii mevalonate + CoA + NAD+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.88 9
-
 oxidative acylation of mevalonate Pseudomonas mevalonii
1.1.1.88 9.5
-
 H381N mutant, oxidative acylation of mevalonate Pseudomonas mevalonii
1.1.1.88 10
-
 H381A mutant, oxidative acylation of mevalonate Pseudomonas mevalonii
1.1.1.88 10.5
-
 H381Q mutant, oxidative acylation of mevalonate Pseudomonas mevalonii
1.1.1.88 11
-
 H381K mutant, oxidative acylation of mevalonate Pseudomonas mevalonii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.88 NAD+
-
 Pseudomonas mevalonii
1.1.1.88 NADH reverse reaction Pseudomonas mevalonii