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Literature summary extracted from

  • Gill, J.F.; Beach, M.J.; Rodwell, V.W.
    Mevalonate utilization in Pseudomonas sp. M. Purification and characterization of an inducible 3-hydroxy-3-methylglutaryl coenzyme A reductase (1985), J. Biol. Chem., 260, 9393-9398.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.88 4-vinylpyridine, 20 min, 0°C, 76% activity Pseudomonas mevalonii
1.1.1.88 5,5'-dithiobis(2-nitrobenzoic acid), 20 min, 0°C, 20% activity Pseudomonas mevalonii
1.1.1.88 N-ethylmaleimide, 20 min, 0°C, 0% activity Pseudomonas mevalonii
1.1.1.88 p-hydroxymercuribenzoate, 20 min, 0°C, 19% activity Pseudomonas mevalonii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.88 acetoacetate competivive inhibitor vs. mevalonate, non inhibitory vs. NAD+ Pseudomonas mevalonii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.88 0.15
-
 R-mevalonate oxidative acylation of mevalonate Pseudomonas mevalonii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.88 43000
-
 alpha4, 4 * 43000, SDS-PAGE Pseudomonas mevalonii
1.1.1.88 178000
-
 gel filtration Pseudomonas mevalonii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.88 (S)-3-hydroxy-3-methylglutaryl-CoA + NADH + H+ Pseudomonas mevalonii mevalonate is first commited intermediate in polyisoprenoid synthesis (R)-mevalonate + NAD+ + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.88 Pseudomonas mevalonii
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.88 60.5
-
 mevalonate acylation Pseudomonas mevalonii

Storage Stability

EC Number Storage Stability Organism
1.1.1.88 0°C, 10 hours, 5% loss of activity Pseudomonas mevalonii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.88 (R)-mevalonate + CoA + 2 NAD+
-
 Pseudomonas mevalonii (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+
-
 r
1.1.1.88 (S)-3-hydroxy-3-methylglutaryl-CoA + NADH + H+ mevalonate is first commited intermediate in polyisoprenoid synthesis Pseudomonas mevalonii (R)-mevalonate + NAD+ + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.88 tetramer alpha4, 4 * 43000, SDS-PAGE Pseudomonas mevalonii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.88 40
-
 oxidative acylation of mevalonate Pseudomonas mevalonii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.88 8.1
-
 oxidative acylation of mevalonate Pseudomonas mevalonii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.88 NAD+
-
 Pseudomonas mevalonii
1.1.1.88 NADH reverse reaction Pseudomonas mevalonii