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Literature summary extracted from

  • Bensch, W.R.; Rodwell, V.W.
    Purification and properties of 3-hydroxy-3-methylglutaryl coenzyme A reductase from Pseudomonas (1970), J. Biol. Chem., 245, 3755-3762.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.88 dithiothreitol causes loss of activity Pseudomonas mevalonii
1.1.1.88 EDTA causes loss of activity Pseudomonas mevalonii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.88 3-hydroxy-3-methylglutarate competitive inhibitor Pseudomonas mevalonii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.88 0.032
-
 NADH reductive deacylation to mevalonate Pseudomonas mevalonii
1.1.1.88 0.039
-
 CoA oxidative acylation of mevalonate Pseudomonas mevalonii
1.1.1.88 0.046
-
 CoA mevaldate oxidation Pseudomonas mevalonii
1.1.1.88 0.05
-
 DL-3-hydroxy-3-methylglutaryl-CoA reductive deacylation to mevalonate Pseudomonas mevalonii
1.1.1.88 0.06
-
 NAD+ mevaldate oxidation Pseudomonas mevalonii
1.1.1.88 0.27
-
 NAD+ oxidative acylation of mevalonate Pseudomonas mevalonii
1.1.1.88 0.3
-
 mevalonate oxidative acylation of mevalonate Pseudomonas mevalonii
1.1.1.88 0.36
-
 NADH mevaldate reduction to mevalonate Pseudomonas mevalonii
1.1.1.88 0.61
-
 DL-mevaldate mevaldate oxidation Pseudomonas mevalonii
1.1.1.88 0.61
-
 DL-mevaldate oxidative acylation of DL-mevaldate Pseudomonas mevalonii
1.1.1.88 9
-
 DL-mevaldate mevaldate reduction to mevalonate Pseudomonas mevalonii
1.1.1.88 9
-
 DL-mevaldate DL-mevaldate reduction Pseudomonas mevalonii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.88 cytosol
-
 Pseudomonas mevalonii 5829
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.88 Pseudomonas mevalonii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.88 (R)-mevalonate + CoA + 2 NAD+
-
 Pseudomonas mevalonii (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+
-
 r
1.1.1.88 3-hydroxy-3-methylglutaryl-CoA + NADH + H+
-
 Pseudomonas mevalonii mevalonate + CoA + NAD+
-
 ?
1.1.1.88 DL-mevaldate + CoA + NAD+
-
 Pseudomonas mevalonii 3-hydroxy-3-methylglutaryl-CoA NADH + H+
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.88 9.2 9.6 oxidative acylation of mevalonate Pseudomonas mevalonii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.88 NAD+
-
 Pseudomonas mevalonii
1.1.1.88 NADH reverse reaction Pseudomonas mevalonii