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Literature summary extracted from

  • Hirasawa, M.; Ruelland, E.; Schepens, I.; Issakidis-Bourguet, E.; Miginiac-Maslow, M.; Knaff, D.B.
    Oxidation-reduction properties of the regulatory disulfides of sorghum chloroplast nicotinamide adenine dinucleotide phosphate-malate dehydrogenase (2000), Biochemistry, 39, 3344-3350.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.82 additional information activation by light Sorghum bicolor
1.1.1.82 thioredoxin activates Sorghum sp.
1.1.1.82 thioredoxin the N-terminal disulfide formed between C24 and C29 has a more positive oxidation-reduction midpoint potential than the two other disulfides and is thus likely to be the preregulatory disulfide postulated to function in activating the enzyme Sorghum sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.82 C24S/C207A oxidation-reduction midpoint potential is -310 mV at pH 7.0, compared to -330 mV for the wild-type enzyme Sorghum sp.
1.1.1.82 C24S/C29S oxidation-reduction midpoint potential is -280 mV at pH 7.0, compared to -330 mV for the wild-type enzyme Sorghum sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.82 chloroplast
-
 Sorghum bicolor 9507
-
1.1.1.82 chloroplast
-
 Sorghum sp. 9507
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.82 Sorghum bicolor
-
-
-
1.1.1.82 Sorghum sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.82 oxaloacetate + NADPH
-
 Sorghum bicolor (S)-malate + NADP+
-
 ?
1.1.1.82 oxaloacetate + NADPH
-
 Sorghum sp. (S)-malate + NADP+
-
 ?