BRENDA - Enzyme Database

Oxalacetate keto-enol tautomerase. Purification and characterization

Annett, R.G.; Kosicki, G.W.; J. Biol. Chem. 244, 2059-2067 (1969)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.2.2
2-oxoglutarate
-
Sus scrofa
5.3.2.2
acetoacetate
-
Sus scrofa
5.3.2.2
citrate
-
Sus scrofa
5.3.2.2
CuCl2
-
Sus scrofa
5.3.2.2
Diethyloxaloacetate
-
Sus scrofa
5.3.2.2
DL-malic acid
-
Sus scrofa
5.3.2.2
HgCl2
-
Sus scrofa
5.3.2.2
KCl
-
Sus scrofa
5.3.2.2
Lactate
-
Sus scrofa
5.3.2.2
LiCl
-
Sus scrofa
5.3.2.2
malonate
-
Sus scrofa
5.3.2.2
MgCl2
-
Sus scrofa
5.3.2.2
NaCl
-
Sus scrofa
5.3.2.2
PCMB
-
Sus scrofa
5.3.2.2
pyruvate
-
Sus scrofa
5.3.2.2
Urea
-
Sus scrofa
5.3.2.2
ZnCl2
-
Sus scrofa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.2.2
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.2.2
-
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.2.2
kidney
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.3.2.2
5.06
-
-
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.2.2
keto-Oxaloacetate
-
2874
Sus scrofa
Enol-oxaloacetate
-
2874
Sus scrofa
-
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
5.3.2.2
35
-
40% loss of activity after 10 min
Sus scrofa
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
5.3.2.2
3.5
-
quick denaturation below
Sus scrofa
5.3.2.2
4
-
at pH 8 or pH 4 more stable than at values in between
Sus scrofa
5.3.2.2
8
-
at pH 8 or pH 4 more stable than at values in between
Sus scrofa
5.3.2.2
8.5
-
quick denaturation above
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.2.2
2-oxoglutarate
-
Sus scrofa
5.3.2.2
acetoacetate
-
Sus scrofa
5.3.2.2
citrate
-
Sus scrofa
5.3.2.2
CuCl2
-
Sus scrofa
5.3.2.2
Diethyloxaloacetate
-
Sus scrofa
5.3.2.2
DL-malic acid
-
Sus scrofa
5.3.2.2
HgCl2
-
Sus scrofa
5.3.2.2
KCl
-
Sus scrofa
5.3.2.2
Lactate
-
Sus scrofa
5.3.2.2
LiCl
-
Sus scrofa
5.3.2.2
malonate
-
Sus scrofa
5.3.2.2
MgCl2
-
Sus scrofa
5.3.2.2
NaCl
-
Sus scrofa
5.3.2.2
PCMB
-
Sus scrofa
5.3.2.2
pyruvate
-
Sus scrofa
5.3.2.2
Urea
-
Sus scrofa
5.3.2.2
ZnCl2
-
Sus scrofa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.2.2
-
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.2.2
kidney
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.3.2.2
5.06
-
-
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.2.2
keto-Oxaloacetate
-
2874
Sus scrofa
Enol-oxaloacetate
-
2874
Sus scrofa
-
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
5.3.2.2
35
-
40% loss of activity after 10 min
Sus scrofa
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
5.3.2.2
3.5
-
quick denaturation below
Sus scrofa
5.3.2.2
4
-
at pH 8 or pH 4 more stable than at values in between
Sus scrofa
5.3.2.2
8
-
at pH 8 or pH 4 more stable than at values in between
Sus scrofa
5.3.2.2
8.5
-
quick denaturation above
Sus scrofa