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Literature summary extracted from

  • Yoshida, T.; Yamaguchi, K.; Hagishata, T.; Miyata, T.; Tanabe, T.; Toh, H.; Oshiro, T.; Shimao, M.; Itumi, Y.
    Cloning and expression of the gene for hydroxypyruvate reductase (D-glycerate dehydrogenase) from an obligate methylotroph Hyphomicrobium methyovorum GM2 (1994), Eur. J. Biochem., 223, 727-732.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.81 expressed in Escherichia coli Hyphomicrobium methylovorum GM2

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.81 0.055
-
 NADH native enzyme Hyphomicrobium methylovorum GM2
1.1.1.81 0.064
-
 NADH purified recombinant enzyme Hyphomicrobium methylovorum GM2
1.1.1.81 0.175
-
 Hydroxypyruvate native enzyme Hyphomicrobium methylovorum GM2
1.1.1.81 0.189
-
 Hydroxypyruvate purified recombinant enzyme Hyphomicrobium methylovorum GM2

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.81 hydroxypyruvate + NAD(P)H Hyphomicrobium methylovorum GM2
-
 D-glycerate + NAD(P)+ + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.81 Hyphomicrobium methylovorum GM2
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.81 943
-
-
 Hyphomicrobium methylovorum GM2

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.81 3-hydroxypyruvate + NADH + H+
-
 Hyphomicrobium methylovorum GM2 D-glycerate + NAD+
-
 r
1.1.1.81 hydroxypyruvate + NAD(P)H
-
 Hyphomicrobium methylovorum GM2 D-glycerate + NAD(P)+ + H+
-
 ?
1.1.1.81 hydroxypyruvate + NADH + H+
-
 Hyphomicrobium methylovorum GM2 D-glycerate + NAD+
-
 r