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Literature summary extracted from
- Purification and characterization of glycerol-3-phosphate dehydrogenase (NAD+) in the salt-tolerant yeast Debaryomyces hansenii (1990), Biochim. Biophys. Acta, 1034, 180-185.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.8 | dipotassium malate | slight activation at low ionic strength | Debaryomyces hansenii |  |
| 1.1.1.8 | potassium glutamate | up to 0.1 M, activation, high activity also at high ionic strength | Debaryomyces hansenii | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.8 | 20% polyethylene glycol stabilizes during purification | Debaryomyces hansenii |
| 1.1.1.8 | substrates stabilize dilute preparation | Debaryomyces hansenii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.8 | glycerol | slight inhibition | Debaryomyces hansenii | |
| 1.1.1.8 | KCl | inactivation | Debaryomyces hansenii | |
| 1.1.1.8 | malate | at high concentration | Debaryomyces hansenii | |
| 1.1.1.8 | NaCl | inactivation | Debaryomyces hansenii | |
| 1.1.1.8 | NAD+ | competitive inhibitor to NADH at physiological concentration | Debaryomyces hansenii | |
| 1.1.1.8 | SO42- | at high concentration, MgSO4 most inhibitory | Debaryomyces hansenii | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.8 | 0.0066 | - |
NADH | standard deviation 0.003 mM | Debaryomyces hansenii | |
| 1.1.1.8 | 0.13 | - |
dihydroxyacetone phosphate | standard deviation 0.03 mM | Debaryomyces hansenii | |
| 1.1.1.8 | 0.3 | - |
NAD+ | standard deviation 0.06 mM | Debaryomyces hansenii | |
| 1.1.1.8 | 1.2 | - |
DL-glycerol-3-phosphate | standard deviation 0.5 mM | Debaryomyces hansenii | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.8 | KCl | 0.1-0.3 M, chloride salts become strongly inhibitory at higher concentrations, replacing K+ with Na+ or NH4+yields similar results | Debaryomyces hansenii | |
| 1.1.1.8 | MgCl2 | 0.1-0.3 M | Debaryomyces hansenii | |
| 1.1.1.8 | MgSO4 | 0.1-0.3 M, slight activation at low ionic strength, most inhibitory at higher concentration | Debaryomyces hansenii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.8 | 42000 | - |
x * 42000, SDS-PAGE | Debaryomyces hansenii |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | Debaryomyces hansenii | - |
dihydroxyacetone phosphate + NADH + H+ | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.8 | Debaryomyces hansenii | - |
van Rij strain 26, salt-tolerant | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.8 | 2 isozymes | Debaryomyces hansenii |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH + H+ | ordered ternary-complex mechanism, NADH binds first | Debaryomyces hansenii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.8 | 200 | - |
- |
Debaryomyces hansenii |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.8 | 0°C, 30 mM Tris-H2SO4, 2.5 mM Na2EDTA, 5 mM dithiothreitol, 0,05% NaN3 (pH 8, 4°C), 0.1 mM phenylmethylsulfonylfluoride, 15-17.5% w/v poly(ethylene glycol) 4000, after six days loss of activity insignificant | Debaryomyces hansenii |
| 1.1.1.8 | 0°C, crude extract stable for 6 days in presence of polyethylene glycol | Debaryomyces hansenii |
| 1.1.1.8 | 4°C, stable | Debaryomyces hansenii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.8 | additional information | glyceraldehyde-3-phosphate | Debaryomyces hansenii | ? | - |
? | |
| 1.1.1.8 | additional information | no substrates: dihydroxyacetone | Debaryomyces hansenii | ? | - |
? | |
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | - |
Debaryomyces hansenii | dihydroxyacetone phosphate + NADH + H+ | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.8 | ? | x * 42000, SDS-PAGE | Debaryomyces hansenii |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.8 | 7.9 | 8.2 | maximum activity in pH interval 7.9-8.2 | Debaryomyces hansenii |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.8 | additional information | no activity with NADPH | Debaryomyces hansenii | |
| 1.1.1.8 | NAD+ | - |
Debaryomyces hansenii | |
| 1.1.1.8 | NADH | - |
Debaryomyces hansenii | |
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