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Literature summary extracted from

  • McGinnis, J.F.; DeVellis, J.
    Purification and characterization of rat brain glycerol phosphate dehydrogenase (1974), Biochim. Biophys. Acta, 364, 17-27.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.8 2-mercaptoethanol stabilizes during purification Rattus norvegicus
1.1.1.8 ammonium sulfate stabilizes Rattus norvegicus
1.1.1.8 bovine serum albumin stabilizes Rattus norvegicus
1.1.1.8 lyophilization inactivates Rattus norvegicus
1.1.1.8 phosphate buffer stabilizes Rattus norvegicus
1.1.1.8 repeated freezing and thawing results in rapid loss of activity, to some extent restorable at room temperature Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.8 iodoacetate reversible by dithiothreitol, 10 mM inhibits to 50% Rattus norvegicus
1.1.1.8 N-ethylmaleimide reversible by dithiothreitol, 5 mM inhibits more than 90% of the enzyme activity Rattus norvegicus
1.1.1.8 p-chloromercuribenzoate 10 nM, strong inhibition; reversible by dithiothreitol, 0.001 mM inhibits more than 90% of the enzyme activity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.8 0.01
-
 NADH less than 0.01 mM Rattus norvegicus
1.1.1.8 0.03
-
 NAD+
-
 Rattus norvegicus
1.1.1.8 0.17
-
 dihydroxyacetone phosphate
-
 Rattus norvegicus
1.1.1.8 0.3
-
 alpha-glycerol phosphate
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.8 72000
-
 gel filtration Rattus norvegicus
1.1.1.8 75000
-
 sucrose density gradient ultracentrifugation Rattus norvegicus
1.1.1.8 78000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+ Rattus norvegicus
-
 dihydroxyacetone phosphate + NADH + H+
-
 r
1.1.1.8 sn-glycerol 3-phosphate + NAD+ Rattus norvegicus Sprague-Dawley
-
 dihydroxyacetone phosphate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.8 Rattus norvegicus
-
 Sprague-Dawley
-
1.1.1.8 Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.8 ion-exchange chromatography combined with affinity elution, 2 isozymes that differ in charge by analytical PAGE Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.8 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.8 152.4
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
 Rattus norvegicus dihydroxyacetone phosphate + NADH + H+
-
 r
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
 Rattus norvegicus Sprague-Dawley dihydroxyacetone phosphate + NADH + H+
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.8 45
-
 at least 5 min stable Rattus norvegicus
1.1.1.8 50
-
 rapid denaturation, prevented by 0.1 mg/ml bovine serum albumin, by over 20% ammonium sulfate or by 0.1 M phosphate buffer, pH 7.5 Rattus norvegicus
1.1.1.8 60
-
 inactivation after 5 min Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.8 7.2
-
 reduction of dihydroxyacetone phosphate, purified enzyme, decline in activity is more rapid at higher pH values, 7.3-7.5 crude homogenate of enzyme Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.8 6.5 8.2 about half-maximal activity at pH 6.5 and 8.2, reduction of dihydroxyacetone phosphate Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.8 additional information
-
 more stable at alkaline pH, more stable in phosphate buffer than in Tris-glycine buffer Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.8 NAD+
-
 Rattus norvegicus
1.1.1.8 NADH
-
 Rattus norvegicus