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Literature summary extracted from

  • Fukuda, H.; Moriguchi, M.; Kimura, A.; Tochikura, T.
    Purification and properties of glyoxylate reductase from Neurospora crassa (1981), Agric. Biol. Chem., 45, 1153-1158.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.79 nitrate 25 mM, competitive vs. glyoxylate Neurospora crassa
1.1.1.79 p-chloromercuribenzoate 1 mM, 70% inhibition Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.79 0.0031
-
 NADPH
-
 Neurospora crassa
1.1.1.79 0.085
-
 Hydroxypyruvate
-
 Neurospora crassa
1.1.1.79 0.23
-
 NADH
-
 Neurospora crassa
1.1.1.79 5.7
-
 glyoxylate
-
 Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.79 110000
-
 gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.79 glyoxylate + NAD(P)H Neurospora crassa
-
 glycolate + NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.79 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.79 isoenzyme 2, protamine sulfate, DEAE-cellulose, hydroxylapatite, Sephadex G-150, DEAE-cellulose, phospho-cellulose Neurospora crassa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.79 108.1
-
 isoenzyme 2 Neurospora crassa

Storage Stability

EC Number Storage Stability Organism
1.1.1.79 4°C, alkaline pH, no loss of activity after 1 week, at pH 7-11, 20% loss of activity Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.79 glyoxylate + NAD(P)H
-
 Neurospora crassa glycolate + NAD(P)+
-
 ?
1.1.1.79 glyoxylate + NAD(P)H isoenzyme 2, 16% activity with NADH Neurospora crassa glycolate + NAD(P)+
-
 ?
1.1.1.79 glyoxylate + NADH + H+
-
 Neurospora crassa glycolate + NAD+
-
 r
1.1.1.79 hydroxypyruvate + NAD(P)H + H+ 25 mM hydroxypyruvate, 68% of the activity with glyoxylate, 2.5 mM hydroxypyruvate, isoenzyme 2, 332% of the activity of glyoxylate Neurospora crassa glycerate + NAD(P)+
-
 ?
1.1.1.79 phenylpyruvate + NAD(P)H isoenzyme 2, 6% activity of glyoxylate Neurospora crassa phenyllactate + NAD(P)+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.79 50
-
 60% loss of activity after 10 min, activity is completely lost above 60°C Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.79 6.2
-
 reduction of hydroxypyruvate Neurospora crassa
1.1.1.79 6.7
-
 reduction of glyoxylate Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.79 NAD(P)H NADPH is preferred Neurospora crassa
1.1.1.79 NADPH NADPH is preferred Neurospora crassa